Biofilm and Quorum sensing mediated pathogenicity in Pseudomonas aeruginosa

Brindhadevi, Kathirvel; LewisOscar, Felix; Mylonakis, Eleftherios; Shanmugam, Sabarathinam; Verma, Tikendra Nath; Pugazhendhi, Arivalagan

doi:10.1016/j.procbio.2020.06.001

Cited by 123 publications

(79 citation statements)

References 72 publications

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“…Indeed, cleavage of DNA by DNase decreases the structural integrity of such biofilms [ 65 ]. Additionally, there is a chance of interaction with other biofilm matrix components that influence greatly to the structural integrity of it, reducing more than just eDNA biofilm viscoelasticity and even interfering with the Quorum Sensing pathways [ 66 , 67 ]. As the Pseudomonas biofilm matrix is comprised predominantly of anionic macromolecules such as exopolysaccharides [ 68 ], the introduction of cationic chitosan alters the biofilm electrostatics and potentially enhances the antibacterial activities of NQo.…”

Section: Discussionmentioning

confidence: 99%

Antimicrobial and Antibiofilm Capacity of Chitosan Nanoparticles against Wild Type Strain of Pseudomonas sp. Isolated from Milk of Cows Diagnosed with Bovine Mastitis

et al. 2020

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Bovine mastitis (BM) is the most prevalent bacterial infection in the livestock sector, affecting the dairy industry greatly. The prevention and treatment of this disease is mainly made via antibiotics, but the increasing antimicrobial resistance of pathogens has affected the efficiency of conventional drugs. Pseudomonas sp. is one of the pathogens involved in this infection. The therapeutic rate of cure for this environmental mastitis-causing pathogen is practically zero, regardless of treatment. Biofilm formation has been one of the main virulence mechanisms of Pseudomonas hence presenting resistance to antibiotic therapy. We have manufactured chitosan nanoparticles (NQo) with tripolyphosphate (TPP) using ionotropic gelation. These NQo were confronted against a Pseudomonas sp. strain isolated from milk samples of cows diagnosed with BM, to evaluate their antimicrobial and antibiofilm capacity. The NQo showed great antibacterial effect in the minimum inhibitory concentrations (MIC), minimum bactericidal concentration (MBC) and disk diffusion assays. Using sub lethal concentrations, NQo were tested for inhibition of biofilm formation. The results show that the nanoparticles exhibited biofilm inhibition and were capable of eradicate pre-existing mature biofilm. These findings indicate that the NQo could act as a potential alternative to antibiotic treatment of BM.

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Section: Discussionmentioning

confidence: 99%

Antimicrobial and Antibiofilm Capacity of Chitosan Nanoparticles against Wild Type Strain of Pseudomonas sp. Isolated from Milk of Cows Diagnosed with Bovine Mastitis

et al. 2020

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“…Mostly, roles of QS are classified into four kinds such as (1) Cell maintenance and division (exoenzymes and siderophores production), (2) Horizontal gene transfer (conjugation), (3) Host-pathogen interactions (antibiotic and bioluminescence production), and most importantly (4) Behavior (movement and biofilm formation). The QS mediated biofilm formation has well documented in several Gram-negative and Gram-positive bacterial pathogens ( Carniol and Gilmore, 2004 ; Labbate et al, 2004 ; Kong et al, 2006 ; Longo et al, 2014 ; Brindhadevi et al, 2020 ).…”

Section: Biofilm Formationmentioning

confidence: 99%

Bacterial Biofilm Inhibition: A Focused Review on Recent Therapeutic Strategies for Combating the Biofilm Mediated Infections

Ramanathan

Santhakumari

Poonguzhali³

et al. 2021

Front. Microbiol.

182

109

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Biofilm formation is a major concern in various sectors and cause severe problems to public health, medicine, and industry. Bacterial biofilm formation is a major persistent threat, as it increases morbidity and mortality, thereby imposing heavy economic pressure on the healthcare sector. Bacterial biofilms also strengthen biofouling, affecting shipping functions, and the offshore industries in their natural environment. Besides, they accomplish harsh roles in the corrosion of pipelines in industries. At biofilm state, bacterial pathogens are significantly resistant to external attack like antibiotics, chemicals, disinfectants, etc. Within a cell, they are insensitive to drugs and host immune responses. The development of intact biofilms is very critical for the spreading and persistence of bacterial infections in the host. Further, bacteria form biofilms on every probable substratum, and their infections have been found in plants, livestock, and humans. The advent of novel strategies for treating and preventing biofilm formation has gained a great deal of attention. To prevent the development of resistant mutants, a feasible technique that may target adhesive properties without affecting the bacterial vitality is needed. This stimulated research is a rapidly growing field for applicable control measures to prevent biofilm formation. Therefore, this review discusses the current understanding of antibiotic resistance mechanisms in bacterial biofilm and intensely emphasized the novel therapeutic strategies for combating biofilm mediated infections. The forthcoming experimental studies will focus on these recent therapeutic strategies that may lead to the development of effective biofilm inhibitors than conventional treatments.

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“…O biofilme é um fator de virulência importante de Pseudomonas aeruginosa, pois durante a infecção ele protege a população bacteriana contra o ataque das células fagocitárias do hospedeiro e da ação dos antimicrobianos (Taylor;Yeung & Hancock, 2014;Brindhadevi, 2020), que podem se formar em superfícies bióticas estando relacionadas a infecções locais ou sistêmicas e, superfícies abióticas, como dispositivos médicos (Rybtke et al, 2015).…”

Section: Biofilme De Pseudomonas Aeruginosaunclassified

“…Este é formado por três sistemas de comunicação las, rhl e pqs, que são controlados por moléculas autoindutoras como: N-3-oxo-dodecanoil homoserina lactona, N-butanoil-homoserina lactona e 2-heptil-3-hidroxi-4-quinolona (Kariminik, Baseri-Salehi & Kheirkhah, 2017). Que são responsáveis pela expressão de fatores de virulência, como: raminolipídeos, piocianina, alginato e elastase, que agem lesionando o tecido do hospedeiro, driblando o sistema imunológico e impedindo a fagocitose, além de proteger as células bacterianas da ação de agentes antimicrobianos (Jimenez et al, 2012;Mukherjee et al, 2018;Brindhadevi, 2020).…”

Section: Introductionunclassified

A influência do quórum sensing na formação do biofilme por Pseudomonas aeruginosa

Neves

Nunes²,

Rocha

et al. 2021

RSD

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As bactérias se organizam de forma agregada numa matriz extracelular, chamada biofilme, estrutura que confere proteção a bactéria a ação dos antimicrobianos e a resposta imune do hospedeiro. Assim, Pseudomonas aeruginosa, é classificado como um micro-organismo oportunista, responsável por causar altos números de infecções hospitalares devido a resistência bacteriana desenvolvida por fatores de virulência como o biofilme, controlados por o sistema quorum sensing. Desse modo, o objetivo desse trabalho foi descrever a comunicação das células bacteriana para formação do biofilme por P. aeruginosa, durante o processo de colonização e infecção no hospedeiro. Seguiu-se a metodologia de uma revisão narrativa, com base nos artigos publicados entre os anos de 2000 e 2020, indexados na Biblioteca Virtual em Saúde (BVS), utilizando para busca os descritores: “quorum sensing”, "Pseudomonas aeruginosa", "biofilm", "virulence factors", "Flagella", "pili", "bacterial adhesion" "polysaccharide" "adhesins" e "biofilm matrix”. Foram selecionados artigos publicados na íntegra, em inglês, entre os anos de 1990 e 2020. Foram excluídos artigos incompletos, duplicados e trabalhos acadêmicos como teses e dissertações. Evidenciou-se que a resistência bacteriana de P. aeruginosa aos antibióticos está relacionada a sua alta capacidade de adaptação a ambientes hostis e aos mecanismos de resistência desenvolvidos pela espécie, especialmente a formação do biofilme bacteriano pelo sistema quorum sensing a partir da biossíntese de moléculas autoindutoras como: N-3-oxo-dodecanoil homoserina lactona, N-butanoil-homoserina lactona e 2-heptil-3-hidroxi-4-quinolona, responsáveis por mediar a produção dos fatores de virulência. Esta revisão abordou os aspectos gerais que envolve a patogenicidade oriunda da comunicação bacteriana durante o seu processo de colonização.

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Biofilm and Quorum sensing mediated pathogenicity in Pseudomonas aeruginosa

Cited by 123 publications

References 72 publications

Antimicrobial and Antibiofilm Capacity of Chitosan Nanoparticles against Wild Type Strain of Pseudomonas sp. Isolated from Milk of Cows Diagnosed with Bovine Mastitis

Antimicrobial and Antibiofilm Capacity of Chitosan Nanoparticles against Wild Type Strain of Pseudomonas sp. Isolated from Milk of Cows Diagnosed with Bovine Mastitis

Bacterial Biofilm Inhibition: A Focused Review on Recent Therapeutic Strategies for Combating the Biofilm Mediated Infections

A influência do quórum sensing na formação do biofilme por Pseudomonas aeruginosa

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