Biofabrication of zinc oxide nanoparticles from Melia azedarach and its potential in controlling soybean seed-borne phytopathogenic fungi

Lakshmeesha, T.R.; Murali, M.; Ansari, Mohammad Azam; Udayashankar, A. C.; Alzohairy, Mohammad A.; Almatroudi, Ahmad; Alomary, Mohammad N.; Asiri, Sarah Mousa; Ashwini, Bagepalli Shivaram; Kalagatur, Naveen Kumar; Nayak, Chandra; Niranjana, S. R.

doi:10.1016/j.sjbs.2020.06.013

Cited by 49 publications

(8 citation statements)

References 47 publications

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“…Abdel-kareem and Ahmed Zohri (2017) have demonstrated the application of SeNPs to limit the growth of A. parasiticus, A. ochraceus, and A. nidulans, as well as their mycotoxins aflatoxin, ochratoxin A, and sterigmatocystin, and reported high doses of SeNPs (3,000-9,000 µg/ml and 800-2,000 µg/ml, respectively) for regulating growth and mycotoxins. However, the majority of research has been done with different nanoparticles for the controlling of mycotoxins, such as zinc oxide, silver, and iron oxide (Lakshmeesha et al, 2019(Lakshmeesha et al, , 2020. However, substantial research is needed to reveal the molecular metabolic pathways that drive nanoparticle-induced antifungal and antimycotoxin action.…”

Section: Antimycotoxin Activity Of Selenium Nanoparticlesmentioning

confidence: 99%

Multi-Biofunctional Properties of Phytofabricated Selenium Nanoparticles From Carica papaya Fruit Extract: Antioxidant, Antimicrobial, Antimycotoxin, Anticancer, and Biocompatibility

Vundela

Kalagatur²,

Nagaraj

et al. 2022

Front. Microbiol.

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The present study focused on phytofabrication of selenium nanoparticles (SeNPs) from Carica papaya extract and exploration of their multi-biofunctional features. Total phenolics and flavonoids of C. papaya fruit extract were determined as 23.30 ± 1.88 mg gallic acid equivalents and 19.21 ± 0.44 mg quercetin equivalents per gram, respectively, which suggested that C. papaya fruit extract could be a competitive reducing and stabilizing agent during phytofabrication of nanoparticles. UV–Vis and FTIR spectroscopy showed the formation of SeNPs from sodium selenite, which could be related to the reducing and stabilizing activities of C. papaya fruit extract. The SeNPs were found to be stable with a Zeta potential of −32 mV. The average hydrodynamic size of SeNPs was found as 159 nm by dynamic light scattering. The SeNPs showed a broader XRD pattern with no sharp Bragg’s peaks and found to be amorphous. SEM showed that SeNPs were spherical in shape and EDX pattern showed that SeNPs were made up of Se (71.81%), C (11.41%), and O (14.88%). The HR-TEM picture showed that SeNPs were spherical in morphology and have a size range of 101–137 nm. The SeNPs exhibited potent antioxidant activity and their EC50 values (effective concentration required to inhibit 50% of radicals) were 45.65 ± 2.01 and 43.06 ± 3.80 μg/ml in DPPH and ABTS assays, respectively. The antimicrobial action of SeNPs was found as a broad spectrum and suppressed microbial pathogens in ascending order: fungi > Gram-positive bacteria > Gram-negative bacteria. The SeNPs have been demonstrated to reduce the growth and ochratoxin A (OTA) of mycotoxigenic Aspergillus ochraceus and Penicillium verrucosum at 40 μg/ml in broth culture, which is noteworthy. The SeNPs reduced cancer cell proliferation (RAW 264.7, Caco-2, MCF-7, and IMR-32) more preferentially than normal cells (Vero), found to be highly biocompatible. Lower doses of SeNPs (up to 50 μg/ml) were shown to be less toxic and did not cause death in Danio rerio (zebrafish) embryos, implying that lower doses of SeNPs could be beneficial for biological purposes. The present study concluded that phytofabricated SeNPs have multiple biofunctional properties, including antioxidant, antimicrobial, antimycotoxin, and anticancer activities, as well as high biocompatibility.

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Section: Antimycotoxin Activity Of Selenium Nanoparticlesmentioning

confidence: 99%

Multi-Biofunctional Properties of Phytofabricated Selenium Nanoparticles From Carica papaya Fruit Extract: Antioxidant, Antimicrobial, Antimycotoxin, Anticancer, and Biocompatibility

Vundela

Kalagatur²,

Nagaraj

et al. 2022

Front. Microbiol.

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“…Reactive oxygen species seem to play a major role in antifungal activity. By inducing oxidative stress, they can destroy all biomolecules in the cell (including proteins and DNA) and disrupt the function of intracellular organs [31]. Nanostructures can also cause fungal death by damaging cellular enzymes and disrupting the electron transfer chain (Figure 1).…”

Section: Resultsmentioning

confidence: 99%

Optimized Synthesis of Xanthan gum/ZnO/TiO₂Nanocomposite with High Antifungal Activity against Pathogenic Candida albicans

Ghorbani

Gorji

Mobarakeh

et al. 2022

Journal of Nanomaterials

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Increased resistance of fungal pathogens to common antimicrobial agents is known as one of the most important human problems. Due to the limited variety of antifungal drugs available, the identification and use of new antifungal drugs are essential. This study aimed to determine the optimal conditions for synthesizing a novel nanocomposite of xanthan gum/ZnO/TiO2with the highest antifungal activity against Candida albicans (C. albicans). For this purpose, nine experiments were designed using the Taguchi method. In the designed experiments, three factors of xanthan gum, ZnO, and TiO2nanoparticles have been investigated at three different levels, and the best ratio with the highest antifungal activity was determined. The results showed that in the presence of the synthesized nanocomposite in experiment 3 (xanthan gum 0.01 M, ZnO 0.09 M, and TiO2 0.09 M), the inhibition of fungal growth reached 92.51%. The properties of the synthesized nanocomposite and its components were investigated using different characterization methods, which confirmed the formation of nanocomposites with desirable properties. The antifungal activity results showed that the synthesized nanocomposite as an antifungal agent has an effective performance and can be used well in various fields.

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“…Transmission electron microscopy (TEM) images were obtained using a PHILIPS CM300 (Nederland) instrument at an accelerating voltage of 100 kV. The preparation of samples was done by dropping 20 μl of CED‐ZnO NPs on a copper grid (200 mesh) and then drying it at room temperature (Khan, Shahid, Hanif, et al, 2021; Khan, Shahid, Ayaz, et al, 2021; Lakshmeesha et al, 2020).…”

Section: Methodsmentioning

confidence: 99%

Desertifilum sp. EAZ03 cell extract as a novel natural source for the biosynthesis of zinc oxide nanoparticles and antibacterial, anticancer and antibiofilm characteristics of synthesized zinc oxide nanoparticles

Ebadi

Zolfaghari

Aghaei

et al. 2021

J of Applied Microbiology

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Aims The use of cyanobacterial cell extracts for the synthesis of zinc oxide nanoparticles (ZnO NPs) seems to be superior to other methods of synthesis because of its a green, environmentally friendly and low‐cost approach. In this study, the cell extract of a newly characterized cyanobacterial strain Desertifilum sp. EAZ03 was used for the biosynthesis of ZnO NPs. The antimicrobial, antibiofilm and anticancer activities of the biosynthesized ZnO NPs (hereinafter referred to as CED‐ZnO NPs) were examined as well. Methods and Results UV–Vis spectroscopy analysis of CED‐ZnO NPs showed an absorbance band at 364 nm, and powder X‐ray diffraction analysis confirmed the purity of the synthesized nanoparticles. The analyses of scanning electron microscopy and transmission electron microscopy images revealed that CED‐ZnO NPs were rod‐shaped with a size of 88 nm. The study of the biological features of CED‐ZnO NPs showed a significant antimicrobial potential against the bacterial strains tested. CED‐ZnO NPs were able to impede the biofilm formation by Staphylococcus aureus, Escherichia coli and Pseudomonas aeruginosa up to 80%, 89% and 85%, respectively. The nanoparticles also showed 69%, 70% and 62% degrading activity against S. aureus, E. coli and P. aeruginosa 1‐day‐old biofilms, respectively. The antibiofilm activity of the synthesized nanoparticles was investigated by confocal laser scanning microscopy. The MTT assay showed that CED‐ZnO NPs, at a concentration of 100 μg/ml, had less cytotoxicity towards normal lung (MRC‐5) cells, at the half, compared to cancerous lung alveolar epithelial (A549) cells. The minimum inhibitory concentration and minimum bactericidal concentration values of CED‐ZnO NPs against E. coli, P. aeruginosa and S. aureus were 1500, 2000 and 32 μg/ml, and 2500, 3500 and 64 μg/ml, respectively. Conclusions The multifunctional CED‐ZnO NPs seem to be promising for possible applications in the therapeutic and pharmaceutical industries. Significance and Impact of the Study This study proposes a new approach for the biosynthesis of zinc oxide nanoparticles using a newly characterized cyanobacterial strain Desertifilum sp. EAZ03. The considerable antimicrobial, antibiofilm and anticancer activities of the biosynthesized zinc oxide nanoparticles further emphasize the emerging role of microbial systems in the green synthesis of metal oxide nanoparticles.

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Biofabrication of zinc oxide nanoparticles from Melia azedarach and its potential in controlling soybean seed-borne phytopathogenic fungi

Abstract: Graphical abstract

Cited by 49 publications

References 47 publications

Multi-Biofunctional Properties of Phytofabricated Selenium Nanoparticles From Carica papaya Fruit Extract: Antioxidant, Antimicrobial, Antimycotoxin, Anticancer, and Biocompatibility

Multi-Biofunctional Properties of Phytofabricated Selenium Nanoparticles From Carica papaya Fruit Extract: Antioxidant, Antimicrobial, Antimycotoxin, Anticancer, and Biocompatibility

Optimized Synthesis of Xanthan gum/ZnO/TiO₂Nanocomposite with High Antifungal Activity against Pathogenic Candida albicans

Desertifilum sp. EAZ03 cell extract as a novel natural source for the biosynthesis of zinc oxide nanoparticles and antibacterial, anticancer and antibiofilm characteristics of synthesized zinc oxide nanoparticles

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Biofabrication of zinc oxide nanoparticles from Melia azedarach and its potential in controlling soybean seed-borne phytopathogenic fungi

Abstract: Graphical abstract

Cited by 49 publications

References 47 publications

Multi-Biofunctional Properties of Phytofabricated Selenium Nanoparticles From Carica papaya Fruit Extract: Antioxidant, Antimicrobial, Antimycotoxin, Anticancer, and Biocompatibility

Multi-Biofunctional Properties of Phytofabricated Selenium Nanoparticles From Carica papaya Fruit Extract: Antioxidant, Antimicrobial, Antimycotoxin, Anticancer, and Biocompatibility

Optimized Synthesis of Xanthan gum/ZnO/TiO2Nanocomposite with High Antifungal Activity against Pathogenic Candida albicans

Desertifilum sp. EAZ03 cell extract as a novel natural source for the biosynthesis of zinc oxide nanoparticles and antibacterial, anticancer and antibiofilm characteristics of synthesized zinc oxide nanoparticles

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Optimized Synthesis of Xanthan gum/ZnO/TiO₂Nanocomposite with High Antifungal Activity against Pathogenic Candida albicans