Biofabrication of skin tissue constructs using alginate, gelatin and diethylaminoethyl cellulose bioink

Somasekharan, Lakshmi T.; Raju, Riya; Kumar, S. S. Pradeep; Geevarghese, Rency; Nair, Renjith P.; Kasoju, Naresh; Bhatt, Anugya

doi:10.1016/j.ijbiomac.2021.08.114

Cited by 32 publications

(29 citation statements)

References 46 publications

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“…However, after 72 h the cell viabilities are still high and the cells maintained their typical morphology, as illustrated for the cells exposed to the inks with a higher content of LNFs (Figure 6b). These results were expected, as sodium alginate is highly recognized as a non-cytotoxic biopolymer for different cell lines [25,27], including HaCaT cells [74]. To the best of our knowledge, the cytotoxicity of LNFs towards HaCaT cells has not yet been evaluated; however, other works have proven their non-cytotoxicity towards other skin cell lines, such as L929 fibroblasts [44], also widely used in skin models.…”

Section: In Vitro Cytotoxicity Towards Hacat Cellsmentioning

confidence: 70%

Alginate-Lysozyme Nanofibers Hydrogels with Improved Rheological Behavior, Printability and Biological Properties for 3D Bioprinting Applications

et al. 2022

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In this study, alginate nanocomposite hydrogel bioinks reinforced with lysozyme nanofibers (LNFs) were developed. Alginate-LNF (A-LNF) suspensions with different LNF contents (1, 5 and 10 wt.%) were prepared and pre-crosslinked with 0.5% (w/v) CaCl2 to formulate A-LNF inks. These inks exhibit proper shear-thinning behavior and good recovery properties (~90%), with the pre-crosslinking step playing a crucial role. A-LNF fully crosslinked hydrogels (with 2% (w/v) CaCl2) that mimic 3D printing scaffolds were prepared, and it was observed that the addition of LNFs improved several properties of the hydrogels, such as the morphology, swelling and degradation profiles, and mechanical properties. All formulations are also noncytotoxic towards HaCaT cells. The printing parameters and 3D scaffold model were then optimized, with A-LNF inks showing improved printability. Selected A-LNF inks (A-LNF0 and A-LNF5) were loaded with HaCaT cells (cell density 2 × 106 cells mL−1), and the cell viability within the bioprinted scaffolds was evaluated for 1, 3 and 7 days, with scaffolds printed with the A-LNF5 bioink showing the highest values for 7 days (87.99 ± 1.28%). Hence, A-LNF bioinks exhibited improved rheological performance, printability and biological properties representing a good strategy to overcome the main limitations of alginate-based bioinks.

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Section: In Vitro Cytotoxicity Towards Hacat Cellsmentioning

confidence: 70%

Alginate-Lysozyme Nanofibers Hydrogels with Improved Rheological Behavior, Printability and Biological Properties for 3D Bioprinting Applications

et al. 2022

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“…Among the various seaweed-derived polysaccharides, alginic acid (and particularly its salt form, sodium alginate) [ 55 , 56 , 57 , 58 , 59 , 60 , 61 , 62 , 63 , 64 , 65 , 66 ], carrageenan [ 67 , 68 , 69 , 70 , 71 , 72 , 73 , 74 ], and agarose [ 75 , 76 , 77 , 78 , 79 ] have been widely used as polymeric matrices (either solely or in combination with other polysaccharides or proteins) for the development of hydrogel-based bioinks for 3D bioprinting, as outlined in Table 1 .…”

Section: Polysaccharide-based Hydrogel Bioinksmentioning

confidence: 99%

“…Due to the proven and cumulated knowledge regarding alginate hydrogels bioinks in the latter years, the most important efforts on the development of novel alginate-based bioinks have been essentially centered on creating more sophisticated 3D living scaffolds from alginate that allow the fabrication of both “soft” (skin) [ 57 ] and “hard” (bone and cartilage) [ 55 , 56 ] biomimetic tissue constructs, on engineering vascular structures [ 62 , 65 ] and on the design of disease models [ 58 , 59 ]. On this matter, for instance, Somasekharan et al [ 57 ] reported a bioink based on alginate blended with gelatin and diethylaminoethyl cellulose (DCEL) to produce skin tissue analogues by extrusion bioprinting. The addition of gelatin enhanced the cell adhesion due to the intrinsic presence of RGD peptide sequences on the gelatin backbone, and the incorporation of DCEL provided matrix stability and improved mechanical properties because of its fibrous nature.…”

Section: Polysaccharide-based Hydrogel Bioinksmentioning

confidence: 99%

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A Guide to Polysaccharide-Based Hydrogel Bioinks for 3D Bioprinting Applications

Teixeira

Lameirinhas

Carvalho

et al. 2022

IJMS

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Three-dimensional (3D) bioprinting is an innovative technology in the biomedical field, allowing the fabrication of living constructs through an approach of layer-by-layer deposition of cell-laden inks, the so-called bioinks. An ideal bioink should possess proper mechanical, rheological, chemical, and biological characteristics to ensure high cell viability and the production of tissue constructs with dimensional stability and shape fidelity. Among the several types of bioinks, hydrogels are extremely appealing as they have many similarities with the extracellular matrix, providing a highly hydrated environment for cell proliferation and tunability in terms of mechanical and rheological properties. Hydrogels derived from natural polymers, and polysaccharides, in particular, are an excellent platform to mimic the extracellular matrix, given their low cytotoxicity, high hydrophilicity, and diversity of structures. In fact, polysaccharide-based hydrogels are trendy materials for 3D bioprinting since they are abundant and combine adequate physicochemical and biomimetic features for the development of novel bioinks. Thus, this review portrays the most relevant advances in polysaccharide-based hydrogel bioinks for 3D bioprinting, focusing on the last five years, with emphasis on their properties, advantages, and limitations, considering polysaccharide families classified according to their source, namely from seaweed, higher plants, microbial, and animal (particularly crustaceans) origin.

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“…The bio-ink was reported to be non-cytotoxic and stable, and, upon loading with cells like human fibroblast and keratinocyte, the 3D constructs exhibited increased cell viability, enhanced collagen expression, and other skin-specific markers. Ultimately, it was suggested to be used for the growth of skin tissue equals with separate epidermal-dermal histological characteristics [81]. Hyaluronan-based bio-ink was characterized using two cross-linking mechanisms: (1) enzymatic method to form a soft gel that would be suitable for cell encapsulation and (2) visible light photo-cross-linking to shape 3D constructs.…”

Section: Carbohydrates-based Bio-inkmentioning

confidence: 99%

Three-Dimensional (3D) Printing in Cancer Therapy and Diagnostics: Current Status and Future Perspectives

Safhi

2022

Pharmaceuticals

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Three-dimensional (3D) printing is a technique where the products are printed layer-by-layer via a series of cross-sectional slices with the exact deposition of different cell types and biomaterials based on computer-aided design software. Three-dimensional printing can be divided into several approaches, such as extrusion-based printing, laser-induced forward transfer-based printing systems, and so on. Bio-ink is a crucial tool necessary for the fabrication of the 3D construct of living tissue in order to mimic the native tissue/cells using 3D printing technology. The formation of 3D software helps in the development of novel drug delivery systems with drug screening potential, as well as 3D constructs of tumor models. Additionally, several complex structures of inner tissues like stroma and channels of different sizes are printed through 3D printing techniques. Three-dimensional printing technology could also be used to develop therapy training simulators for educational purposes so that learners can practice complex surgical procedures. The fabrication of implantable medical devices using 3D printing technology with less risk of infections is receiving increased attention recently. A Cancer-on-a-chip is a microfluidic device that recreates tumor physiology and allows for a continuous supply of nutrients or therapeutic compounds. In this review, based on the recent literature, we have discussed various printing methods for 3D printing and types of bio-inks, and provided information on how 3D printing plays a crucial role in cancer management.

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Biofabrication of skin tissue constructs using alginate, gelatin and diethylaminoethyl cellulose bioink

Cited by 32 publications

References 46 publications

Alginate-Lysozyme Nanofibers Hydrogels with Improved Rheological Behavior, Printability and Biological Properties for 3D Bioprinting Applications

Alginate-Lysozyme Nanofibers Hydrogels with Improved Rheological Behavior, Printability and Biological Properties for 3D Bioprinting Applications

A Guide to Polysaccharide-Based Hydrogel Bioinks for 3D Bioprinting Applications

Three-Dimensional (3D) Printing in Cancer Therapy and Diagnostics: Current Status and Future Perspectives

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