Biofabrication of gold nanoparticles by Lyptolyngbya JSC-1 extract as super reducing and stabilizing agents: Synthesis, characterization and antibacterial activity

Zada, Shah; Ahmad, Aftab; Khan, Sikandar; Iqbal, Arshad; Ahmad, Shahbaz; Ali, Hamid; Fu, Pengcheng

doi:10.1016/j.micpath.2017.11.038

Cited by 37 publications

(19 citation statements)

References 34 publications

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“…Generally, the colour change occurred due to reducing agents released from algal extracts into solution [21]. This was also supported by UV-visible spectra, when exposed to light of specific wavelength, the NPs gave a unique surface phenomenon called surface plasmon resonance (SPR); due to this, a specific peak formation occurred for each kind of NPs by UV-Vis spectroscopy [7]. The synthesis of AgNPs using (Leptolyngbya JSC-1) extract was monitored by using UV-Vis spectroscopy; the light absorption pattern of the algal biomass was observed in the range of 300-700 nm.…”

Section: Synthesis Of Agnps and Uv-visible Spectroscopymentioning

confidence: 79%

“…The probable mechanism of action of the noble metal nanoparticles to eradicate bacteria might be the membrane damage, creation of reactive oxygen species (ROS), inhibition of some essential enzymes and DNA damage, etc. [7]. Among these mechanisms, the most effective was found to be the ROS production and cell membrane disruption leading to leakage of nucleic acids and protein from bacterial cells [16,19].…”

Section: Inhibition Of Bacterial Growth and Antibacterial Activitymentioning

confidence: 99%

“…The use of eco-friendly materials like whole plant, parts of plant, algae and cyanobacteria for the synthesis of nanoparticles (NPs) offer several benefits for environment and compatibility for biomedical applications [5]. Free metal nanoparticles due to their unique physical and chemical properties have potential applications in catalysis, bio-sensing, bio-labelling, electronics, optical devices and controlled drug delivery [6,7].…”

Section: Introductionmentioning

confidence: 99%

“…These bio-nanofactories possess unique ability to synthesize biochemicals, involved in the synthesis of metallic nanoparticles with high stability [11]. Recently, metal nanoparticles were synthesized using the extracts of algae such as Turbinaria conoides, Kappaphycus alvarezii, Laminaria japonica, Chlorella pyrenoidusa, Stoechospermum marginatum, Sargassum wightii, Acanthophora spicifera and Sargassum myriocystum [7]. One of the largest group of algae namely cyanobacteria (bluegreen algae) found in natural aquatic environment were also reported for the same purpose of bio-fabrication of metal nanoparticles [12].…”

Section: Introductionmentioning

confidence: 99%

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Biogenic synthesis of silver nanoparticles using extracts of Leptolyngbya JSC-1 that induce apoptosis in HeLa cell line and exterminate pathogenic bacteria

Zada

Ahmad

Khan

et al. 2018

Artificial Cells, Nanomedicine, and Biotechnology

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Utilizing novel approaches for the green synthesis of metal nanoparticles are of great importance. Therefore, we reported biogenic synthesis of silver nanoparticles (AgNPs) using extracts of Leptolyngbya strain JSC-1, and their significant applications against pathogenic bacteria and cancerous HeLa cell line. The biofabricated AgNPs were characterized by UV-visible spectroscopy, FTIR, SEM, TEM, DLS and zeta-potential. The as prepared AgNPs were assessed for inhibition of bacterial growth and induction of apoptosis in HeLa cells by different doses of AgNPs was evaluated. UV-visible spectroscopy and FTIR of AgNPs demonstrated the surface plasmon resonance at 413 nm and interaction among extract and nanoparticles, respectively. Electron microscopy revealed the morphology and DLS demonstrated size distribution of the particles (10-100 nm). Zeta potential values were between -47 and 0 mV, indicating stability of the particles. Proliferation of HeLa cells was significantly inhibited and severe cytotoxicity with higher intracellular uptake were observed after applying high concentration of AgNPs. Efficient inhibition zones (17 ± 2 and 21 ± 2 mm) were produced at maximum concentration (100 µl from 1 mg ml stock of AgNPs) for Staphylococcus aureus and Escherichia coli, respectively. These findings reveal that the biofabricated AgNPs possess strong antibacterial activity and ability to induce apoptosis in cancer cell line (HeLa).

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Section: Synthesis Of Agnps and Uv-visible Spectroscopymentioning

confidence: 79%

Section: Inhibition Of Bacterial Growth and Antibacterial Activitymentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Biogenic synthesis of silver nanoparticles using extracts of Leptolyngbya JSC-1 that induce apoptosis in HeLa cell line and exterminate pathogenic bacteria

Zada

Ahmad

Khan

et al. 2018

Artificial Cells, Nanomedicine, and Biotechnology

Self Cite

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“…AuNPs synthesized through aforementioned protocols become unfit for medical applications and economically unfeasible due to involvement of hazardous reagents and expensive equipment in their synthesis procedures [14,15]. AuNPs are also being synthesized through biological resources including plants extracts and microbes [16,17]. However, biological synthesis of AuNPs through microorganisms is reported to contain various operation impediments, such as long time required to synthesize nanoparticles, critical conditions for growth of microbes and inflexible process conditions [18].…”

Section: Introductionmentioning

confidence: 99%

Effect of Volume of Gold Chloroauric Acid on Size, Shape and Stability of Biosynthesized AuNPs using Aqueous Elaeis guineensis (Oil Palm) Leaves Extract

Ahmad¹,

Bustam²,

Irfan³

et al. 2018

INT. J. AUTOMOT. MECH. ENG.

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Biosynthesis of gold nanoparticles (AuNPs) using plant extracts has been receiving considerable attention of researchers due to their environmental friendly, low cost and renewability features. AuNPs were successfully synthesized at ambient conditions using aqueous Elaeis guineensis (oil palm) leaves extract without addition of any chemical agent. UV-Vis spectrophotometer, DLS and TEM were used to characterize the phytosynthesized AuNPs. FTIR was carried out to identify the functional groups involved in reduction of gold ions and stabilization of AuNPs. Hydrodynamic diameter was found to be reduced from 69± 39.80 nm to 55.22 ± 42.86 nm with an increase in the volume of gold chloroauric acid (1.53 mM) from 1.0 mL to 2.0 mL with fixed volume of oil palm leaves extract respectively. Size enlargement was observed from 60 ±43.76 nm to 123.5 ±110 nm with further increase in the volume of gold chloroauric acid from 3.0 mL to 5.0 mL respectively. Zeta potential (surface charge) of AuNPs was found to be reduced from-20 ± 6.80 mV to-15 ± 6.15 mV with an increase in the volume of gold chloroauric acid from 1.0 mL to 5.0 mL respectively. TEM results showed the formation of well scattered spherical, triangular and nonspherical shaped AuNPs by using 3.0 mL of gold chloroauric acid with an average particle diameter of 36.84 ± 9.0 nm. FTIR results revealed the role of phenolic, carboxylic and amides functional groups in reduction of gold ions to form AuNPs and their stabilization. Hence, ploydisperse AuNPs synthesized using Elaeis guineensis (oil palm) leaves with alterable morphology and good stability can be efficiently employed in medical and industrial applications.

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Biological Synthesis of Nanoparticles: Algae

Srivastava

Bhargava²

2021

Green Nanoparticles: The Future of Nanobiotechnology

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Algae constitute a polyphyletic and non-cohesive grouping of organisms that are capable of performing photosynthesis using oxygen. Although algae have been utilized as biological sources for the green synthesis of metal nanoparticles in the last few years, their usage has been less as compared to other living forms. The different functional groups and enzymes present in the algal cell walls are thought to play an important role in the fabrication of metal and metal oxide nanoparticles. Nanoparticle production has been reported from all algal groups including bluegreen algae, green algae, diatoms, brown algae, and red algae.

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Biofabrication of gold nanoparticles by Lyptolyngbya JSC-1 extract as super reducing and stabilizing agents: Synthesis, characterization and antibacterial activity

Cited by 37 publications

References 34 publications

Biogenic synthesis of silver nanoparticles using extracts of Leptolyngbya JSC-1 that induce apoptosis in HeLa cell line and exterminate pathogenic bacteria

Biogenic synthesis of silver nanoparticles using extracts of Leptolyngbya JSC-1 that induce apoptosis in HeLa cell line and exterminate pathogenic bacteria

Effect of Volume of Gold Chloroauric Acid on Size, Shape and Stability of Biosynthesized AuNPs using Aqueous Elaeis guineensis (Oil Palm) Leaves Extract

Biological Synthesis of Nanoparticles: Algae

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