Biogenic synthesis of silver nanoparticles using extracts of Leptolyngbya JSC-1 that induce apoptosis in HeLa cell line and exterminate pathogenic bacteria

Abstract

Utilizing novel approaches for the green synthesis of metal nanoparticles are of great importance. Therefore, we reported biogenic synthesis of silver nanoparticles (AgNPs) using extracts of Leptolyngbya strain JSC-1, and their significant applications against pathogenic bacteria and cancerous HeLa cell line. The biofabricated AgNPs were characterized by UV-visible spectroscopy, FTIR, SEM, TEM, DLS and zeta-potential. The as prepared AgNPs were assessed for inhibition of bacterial growth and induction of apoptosis in HeLa cells by different doses of AgNPs was evaluated. UV-visible spectroscopy and FTIR of AgNPs demonstrated the surface plasmon resonance at 413 nm and interaction among extract and nanoparticles, respectively. Electron microscopy revealed the morphology and DLS demonstrated size distribution of the particles (10-100 nm). Zeta potential values were between -47 and 0 mV, indicating stability of the particles. Proliferation of HeLa cells was significantly inhibited and severe cytotoxicity with higher intracellular uptake were observed after applying high concentration of AgNPs. Efficient inhibition zones (17 ± 2 and 21 ± 2 mm) were produced at maximum concentration (100 µl from 1 mg ml stock of AgNPs) for Staphylococcus aureus and Escherichia coli, respectively. These findings reveal that the biofabricated AgNPs possess strong antibacterial activity and ability to induce apoptosis in cancer cell line (HeLa).

Fungus- (Alternaria sp.) Mediated Silver Nanoparticles Synthesis, Characterization, and Screening of Antifungal Activity against Some Phytopathogens

“…e moderate particles' sizes observed were 5, 12, 25, 35, and 50 nm, for AgNPs synthesized by different biological extracts using water as a solvent. Similar results were also found by other researchers recently [16,[25][26][27][28]. In an article comparing the advantages and drawbacks of these methods and the applications of nanoparticles in various domains, a synthesis of chemical, physical, and biological methods for obtaining AgNPs of different shapes and dimensions (from 2 to 300 nm) was described [29].…”

“…Low zeta potential values of particles suggest no flocculation and no tendency to assemble due to repulsion forces among particles [23,24]. e SEM images of AgNPs reported by other researchers from different extracts showed spherical particles, aggregated spherical particles, irregularly shaped particles, and cubic particles [16,25]. e moderate particles' sizes observed were 5, 12, 25, 35, and 50 nm, for AgNPs synthesized by different biological extracts using water as a solvent.…”

Biosynthesis and Antibacterial Activity of Silver Nanoparticles Using Yeast Extract as Reducing and Capping Agents

“…Biomolecules of the yeast extract play a decisive part in the formation of Ag NPs by protecting them from aggregation. Stabilizers of biomolecules help to prevent redundant reactions between Ag NPs [38]. The amphoteric molecules of amino acids contain both basic and acidic groups.…”

<p>Cyanobacteria – A Promising Platform in Green Nanotechnology: A Review on Nanoparticles Fabrication and Their Prospective Applications</p>

“…JSC-1 fabricated Ag-NPs extracellularly from silver nitrate after 20 min. 94,95 A recent study showed that the dispersivity and stability of silver-NPs synthesized using Oscillatoria limnetica changed with increasing time. After 48 h of synthesis, the UV spectrum wavelength value was constant, and after 9 months of incubation, the optical intensity had shifted from 0.71 to 0.599 nm, indicating about the stability of Ag-NPs with low agglomeration.…”

“…37-2-1 31.86 Elongated +, - Light, dark 25 7 72 h Antibacterial agent 55 Lyngbya majuscule CUH/Al/MW-150 20–50 Spherical + Dark RT 4 72 h Antileukemic, antibacterial agent 121 Lyngby asp. — — +,- Dark RT NM 24 h — 68 Leptolyngbya valderianum 2–20 Spherical + Dark RT 3.86 72 h Antibacterial agent 152 Leptolyngbya JSC-1 5–50 Spherical - NM RT NM 20 min Anticancer, antibacterial agent 94 Lyngbya NCCU-102 54 Spherical - Light 30 NM 24 h NM 56 Microchaete NCCU-342 40 …”