Bioevaluation study of 32P-CP-PLLA particle brachytherapy in a rabbit VX2 lung tumor model

Xu, Yu Ping; Yang, Min; Pan, Dong; Wang, Li Zhen; Lü, Ling; Huang, Peilin; Shao, Guoqiang

doi:10.1016/j.apradiso.2011.12.047

Cited by 9 publications

(5 citation statements)

References 18 publications

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“…Our results found that the mean tumor volume drawn in CT images of three consecutive days were not statistically different, which was consistent with previous studies conducted by Xu et al ( 14 ) and Song et al ( 18 ). In 18 F-FDG PET/CT imaging, the lesion with high 18 F-FDG uptake was seen on the vaccination area of the VX2 tumor tissue for every tumor-bearing rabbit.…”

Section: Discussionsupporting

confidence: 93%

Precision of quantitative parameters of 18F-FDG PET/CT in a rabbit VX2 tumor model

Guo¹,

Li²,

Cai³

et al. 2023

Quant Imaging Med Surg

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Background: The precision reflecting repeated measurement error of quantitative parameters of flourine-18 fluorodeoxyglucose positron emission tomography/computed tomography ( 18 F-FDG PET/ CT) for evaluating the therapeutic effect of solid tumor can help observe whether a real biologic change in glucose metabolism occurred, or if the change was caused by errors before and after the treatment.Methods: A total of 18 VX2 tumor-bearing male New Zealand rabbits confirmed by pathology were used, three of which were used for determining the best scanning time point after injection and 15 for a precision experiment by repeating PET/CT scans for three consecutive days. The PET volume computer-assisted reading (PET VCAR) software (GE Healthcare) was used to analyze the standardized uptake value (SUV) and total lesion glycolysis (TLG) parameters. The lean body mass (LBM) to calculate the SUV corrected for lean body mass (SUL) parameters was measured using dual energy X-ray absorptiometry (DXA). The precision was represented as the coefficient of variation of root mean square (RMS-CV) and standard deviation of root mean square (RMS-SD). The least significant change (LSC) was also calculated when considering precision. Results:The precision of SUV parameters, including SUV max , SUV mean and SUV peak ranged from 18.3% to 18.8%, which was similar to that of the SUL parameters (18.0-18.4%). Using 80% confidence interval (CI), the LSC of SUV max and SUL peak were 33.1% and 33.3%, respectively; using 95% CI, the LSC of SUV max and SUL peak were 50.1% and 51.0%, respectively.Conclusions: This research established the method of precision in a rabbit VX2 tumor model, which can be used for monitoring changes to assess the effects of drug treatment on solid tumors in experimental studies with 18 F-FDG PET/CT imaging.

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Section: Discussionsupporting

confidence: 93%

Precision of quantitative parameters of 18F-FDG PET/CT in a rabbit VX2 tumor model

Guo¹,

Li²,

Cai³

et al. 2023

Quant Imaging Med Surg

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“…VX2 tumor cell lines stemmed from the squamous cell carcinoma derived from the papilloma induced by Shope-papilloma virus. Previous methods reported for establishing a VX2 lung cancer rabbit model include percutaneous injection of a VX2 tumor cell suspension with/without CT 29 , 30 and implantation of VX2 tumor tissue fragment under traditional thoracotomy 31 , 32 . However, the former method may result in pleural dissemination and multifocal growth.…”

Section: Discussionmentioning

confidence: 99%

Enhanced antitumor effect on intrapulmonary tumors of docetaxel lung-targeted liposomes in a rabbit model of VX2 orthotopic lung cancer

Lijuan

Che

et al. 2017

Sci Rep

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Allergic reactions and severe systemic toxicity are two major challenges for the clinical application of docetaxel (DTX) for treatment of non-small-cell lung cancer (NSCLC). We developed a novel lung-targeted DTX-loaded liposome (DTX-LP), an efficient drug delivery system, with a patented DBaumNC technology to overcome these deficiencies. In the present study, we describe the targeting activity, tumor inhibition rate (TIR), survival, pathology, tumor apoptosis and metabolism of DTX after intravenous injection of DTX-LP compared to the DTX injection (DTX-IN) formulation based on the VX2 orthotopic lung cancer rabbit model. Biodistribution studies revealed the highest accumulation in lung and tumor within 12 h after the injection of DTX-LP. The increased TIR indicates that the growth of tumor was slowed. Pathology tests demonstrated that DTX-LP can reduce metastasis and toxicity to non-targeted organs, leading to greatly extended survival time and improved survival of tumor-bearing rabbits. Flow cytometry and immunohistochemistry confirmed that DTX-LP is highly efficacious in tumor tissue, leading to a significant increase of tumor apoptosis and decrease of proliferation and angiogenesis. The results from this study demonstrate the increased intrapulmonary tumor targeting activity, enhanced antitumor effect and reduced toxicity of DTX-LP compared to DTX-IN and highlight its clinical prospects for NSCLC therapy.

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“…VX2 tumor-bearing model is a big tumor model and closely imitates human lung cancer initiation, development and progress [ 14 ]. Previous methods reported for the establishment of the VX2 lung cancer rabbit model include the percutaneous injection of VX2 tumor cells suspension with/without computed tomography (CT) [ 15 16 ] and implantation of VX2 tumor tissue fragments under traditional thoracotomy [ 17 18 ]. However, the former method utilizes tumor cells suspension, which may result in pleural dissemination and multifocal growth.…”

Section: Introductionmentioning

confidence: 99%

Establishment and evaluation of the VX2 orthotopic lung cancer rabbit model: a ultra-minimal invasive percutaneous puncture inoculation method

Wang

Che

Liu

et al. 2018

Korean J Physiol Pharmacol

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The purpose of the present work is to establish an ultra-minimal invasive percutaneous puncture inoculation method for a VX2 orthotopic lung cancer rabbit model with fewer technical difficulties, lower mortality of rabbits, a higher success rate and a shorter operation time, to evaluate the growth, metastasis and apoptosis of tumor by CT scans, necropsy, histological examination, flow cytometry and immunohistochemistry. The average inoculation time was 10–15 min per rabbit. The tumor-bearing rate was 100%. More than 90% of the tumor-bearing rabbits showed local solitary tumor with 2–10 mm diameters after two weeks post-inoculation, and the rate of chest seeding was only 8.3% (2/24). The tumors diameters increased to 4–16 mm, and irregularly short thorns were observed 3 weeks after inoculation. Five weeks post-inoculation, the liquefaction necrosis and a cavity developed, and the size of tumor grew further. Before natural death, the CT images showed that the tumors spread to the chest. The flow cytometry and immunohistochemistry indicated that there was less apoptosis in VX2 orthotopic lung cancer rabbit model compared to chemotherapy drug treatment group. Minimal invasive percutaneous puncture inoculation is an easy, fast and accurate method to establish the VX2 orthotopic lung cancer rabbit model, an ideal in situ tumor model similar to human malignant tumor growth.

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Bioevaluation study of 32P-CP-PLLA particle brachytherapy in a rabbit VX2 lung tumor model

Cited by 9 publications

References 18 publications

Precision of quantitative parameters of 18F-FDG PET/CT in a rabbit VX2 tumor model

Precision of quantitative parameters of 18F-FDG PET/CT in a rabbit VX2 tumor model

Enhanced antitumor effect on intrapulmonary tumors of docetaxel lung-targeted liposomes in a rabbit model of VX2 orthotopic lung cancer

Establishment and evaluation of the VX2 orthotopic lung cancer rabbit model: a ultra-minimal invasive percutaneous puncture inoculation method

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