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Background: The objective was to understand the influence of Survivin plasmid with short hairpin RNA (shRNA) on the cell cycle, invasion, and the silencing effect of Survivin gene in the SW480 cell of colorectal carcinoma. Methods:A eukaryotic expression vector, PGCH1/Survivin shRNA, a segment sequence of Survivin as target, was created and transfected into colorectal carcinoma cell line SW480 by the non-lipid method. The influence on the Survivin protein was analyzed by Western blotting, while the cell cycle, cell apoptosis were analyzed by flow cytometry, and invasion of the cell was analyzed by Transwell's chamber method.Results: After the transfection of PGCH1/Survivin shRNA, the expression of Survivin protein in SW480 cells was dramatically decreased by 60.68%, in which the cells were stopped at G2/M phase, even though no apoptosis was detected. The number of transmembranous cells of the experimental group, negative control group, and blank control group were 14. 46 ± 2.11, 25.12 ± 8.37, and 25.86 ± 7.45, respectively (P <0.05). Conclusion:Survivin shRNA could significantly reduce the expression of Survivin protein and invasion of SW480 cells. Changes in cell cycle were observed, but no apoptosis was induced.

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Background To evaluate the early prognosis and management of acute coronary involvement (ACI) in type A aortic dissection (ATAAD) patients without myocardial ischemia (MI). Methods We conducted a retrospective cohort study on a multicenter database. A total of 931 ATAAD patients without MI underwent thoracic aortic surgery between 2018 and 2019 in the Acute Aortic Syndrome Cooperation Network (AASCN) and were enrolled in our study. Patients were divided into two groups: ACI group and non-ACI group. Results There were 139 ACI patients (14.9%) and 792 non-ACI patients (85.1%) in our cohort. ACI group had higher 30-day mortality after surgery than non-ACI group (log-rank test: P = 0.028,Cox regression: hazard ratio [HR], 2.3; 95% confidence interval [95% CI], 1.1–5.39; P = 0.047), especially in sub-group of advanced age (53–80 years; HR, 4.0; 95% CI, 1.3–12.8; P = 0.017), low diastolic blood pressure (29-69 mmHg, HR, 3.8; 95% CI, 1.3–11.2; P = 0.018), low systolic blood pressure (51–119 mmHg, HR, 3.6; 95% CI, 1.1–12.4; P = 0.040), high body mass index (BMI;27.25–47.52 kg/m2; HR, 3.7; 95% CI, 1.3–10.7; P = 0.015) and high hemoglobin (>145 g/L; HR, 4.3; 95% CI, 1.2–16.0; P = 0.030). Acute renal failure was significant more in ACI group than non-ACI group (24.5% vs. 15.9%; P = 0.014). Conclusions ACI increases the short-term postoperative mortality and acute renal failure in ATAAD patients without MI. ATAAD patients with ACI may need a narrower control range of blood pressure even if without myocardial ischemia. Trial registration ChiCTR1900022637. Retrospectively registered 19 April 2019.

Owing to their advantageous physical, chemical, electronic, and optical properties, two-dimensional (2D) materials are widely used as highly active visible-light photocatalysts. In this study, 3D/2D composite photocatalysts were prepared from ammonium phosphotungstate nanoparticles and 2D ultrathin graphitic carbon nitride by an impregnation method and were found to efficiently promote the photodegradation of rhodamine B and tetracycline in water under visible light irradiation. Photocatalytic degradation was maximum at a PW12NH4 loading of 20 wt% (UCN20), with photodegradation efficiencies of 93.40% and 69.6% for RhB and TC, respectively. The photocatalytic degradation rate using UCN20 was 5.8-fold higher for RhB and 2.2-fold higher for TC compared to those with UCN. This high performance is ascribed to the large specific surface areas and pore volumes of the composites and the reduced probability of photogenerated carrier recombination therein. The related degradation mechanism is believed to involve a Z-scheme charge transfer mode, which enhances the redox capability of composite photocatalysts and renders them suitable for the visible light-driven remediation of wastewater containing organic contaminants.