Bioethanol production from wheat straw by the thermotolerant yeast Kluyveromyces marxianus CECT 10875 in a simultaneous saccharification and fermentation fed-batch process

Tomás‐Pejó, Elia; Oliva, J.; González, Alberto; Ballesteros, Ignacio; Ballesteros, Mercedes

doi:10.1016/j.fuel.2009.01.014

Cited by 108 publications

(51 citation statements)

References 34 publications

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“…This is an important characteristic of yeasts with biotechnological potential because temperature is a limiting factor for microbial metabolism and it can reduce the yield of the bioprocess. Furthermore, the use of thermotolerant strains can reduce the waste water, that is required for cooling and permit the use of heat to eliminate contaminant microorganisms (Tomás-Pejó et al 2009). …”

Section: Resultsmentioning

confidence: 99%

Cultivation of Trichosporon mycotoxinivorans in sugarcane bagasse hemicellulosic hydrolyzate

Matos¹,

Cassa-Barbosa²,

Neto³

et al. 2012

Electro Journal of Biotech

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Background: The yeast strain IB09 was isolated from the gut of Calosoma sp. (Carabidae, Coleoptera, Insecta) that were collected in the central Amazon rainforest. First, tolerance of the strain to ethanol and heat was tested. Then, IB09 was cultivated in a medium using sugarcane bagasse hemicellulosic hydrolyzate as a carbon source, and cell growth (OD600), specific growth rate (μMAX, h -1 ), biomass yield (YB, g.g -1 ) and relative sugar consumption (RSC, %) were evaluated. Taxonomic identification was determined by sequencing the ITS1 region of IB09 and comparing it to sequences obtained from the GenBank database (NCBI). Results: IB09 showed both ethanol tolerance and thermotolerance. Relative sugar consumption indicated that IB09 was able to perform saccharification of sugarcane bagasse hemicellulosic hydrolyzate, increasing the total reducing sugar concentration by approximately 50%. The μMAX value obtained was 0,20, indicating that cell growth was slow under the assessed conditions. Biomass yield was 0,701 g per g of consumed sugar, which is relatively high when compared with other findings in the literature. After 120 hrs of cultivation, 80,1% of total reducing sugar had been consumed. Sequencing of the ITS1 region identified IB09 as Trichosporon mycotoxinivorans. Conclusion: This is the first report to document this species in the central Amazon rainforest at this host. Trichosporon mycotoxinivorans has great biotechnological potential for use in the saccharification of sugarcane bagasse hemicellulosic hydrolyzate and for biomass production with this substrate as carbon source.

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Section: Resultsmentioning

confidence: 99%

Cultivation of Trichosporon mycotoxinivorans in sugarcane bagasse hemicellulosic hydrolyzate

Matos¹,

Cassa-Barbosa²,

Neto³

et al. 2012

Electro Journal of Biotech

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“…Among the various pretreatments applied in this work, dilute acid pretreatment is the one that has been largely studied and significantly improves the enzymatic hydrolysis [32,33]. Generally, this pretreatment significantly improved the obtaining of fermentable sugars after enzymatic hydrolysis was applied to the CBM using diluted acid, made with the optimal conditions obtained via experimental design.…”

Section: Enzymatic Hydrolysis Of Pretreated Cellulosic Materialmentioning

confidence: 99%

Study of Chemical and Enzymatic Hydrolysis of Cellulosic Material to Obtain Fermentable Sugars

Amezcua‐Allieri

Durán

Aburto

2017

Journal of Chemistry

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The objective of this study was to evaluate the chemical and enzymatic hydrolysis using a factorial experimental design (2 3 ) in order to obtain fermentable sugars from cellulose-based material (CBM) usually used as pet litter. In assessing chemical hydrolysis, we studied the effect of temperature, in addition to H 2 SO 4 concentration and reaction time, on the production of total sugars, reducing sugars, soluble lignin, carbohydrate profile, furfural (F), and hydroxymethyl furfural (HMF). We performed a response surface analysis and found that, at 100 ∘ C, 1% acid concentration, and 60 min reaction time, the yields of 0.0033 g reducing sugar/g biomass and 0.0852 g total sugars/g biomass were obtained. Under the above conditions, F is not generated, while HMF is generated in such a concentration that does not inhibit fermentation. We pretreated the CBM with H 2 SO 4 , NaOH, CaO, or ozonolysis, in order to evaluate the effectiveness of the enzymatic hydrolysis from the pretreated biomass, using an enzymatic cocktail. Results showed that CBM with acid was susceptible to enzymatic attack, obtaining a concentration of 0.1570 g reducing sugars/g biomass and 0.3798 g total sugars/g biomass. We concluded that acid pretreatment was the best to obtain fermentable sugars from CBM.

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“…The pH of pretreated seaweed slurry was adjusted to 4.5 before the treatment of enzyme mixture of Celluclast 1.5 L (8.4 filter paper unit FPU/ml) and Viscozyme L (1.2 endo-glucanase unit EGU/ml). Enzymatic hydrolysis was carried out at 55 o C in water bath shaker with 120 rpm for 54 h. Enzymatic hydrolyzates were evalulated by reducing sugar contents measurement using DNS method [7]. o C for 120 h and the stirrer speed of 220 rpm.…”

Section: Enzymatic Hydrolysis Of Pretreated Seaweed Slurrymentioning

confidence: 99%

“…Reducing sugar contents were determined by the 3, 5-dinitrosalicyclic acid (DNS) method with glucose (Sigma Chemical Co. USA) as the standard [7]. Sugars, ethanol and other organic acid were analyzed by HPLC (Agilent 1100 Series, Agilent.…”

Section: Analysis Of Reducing Sugar Monosaccharide and Viscositymentioning

confidence: 99%

Ethanol Production from Seaweed, Enteromorpha intestinalis, by Separate Hydrolysis and Fermentation (SHF) and Simultaneous Saccharification and Fermentation (SSF) with Saccharomyces cerevisiae

Cho¹,

Kim²,

Kim³

2013

KSBB Journal

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Ethanol productions were performed by separate hydrolysis and fermentation (SHF) and simultaneous saccharification and fermentation (SSF) processes using seaweed, Enteromorpha intestinalis (sea lettuce). Pretreatment conditions were optimized by the performing thermal acid hydrolysis and enzymatic hydrolysis for the increase of ethanol yield. The pretreatment by thermal acid hydrolysis was carried out with different sulfuric acid concentrations in the range of 25 mM to 75 mM H 2 SO 4 , pretreatment time from 30 to 90 minutes and solid contents of seaweed powder in the range of 101 6% (w/v). Optimal pretreatment conditions were determined as 75 mM H 2 SO 4 and 13% (w/v) slurry at 121 o C for 60 min. For the further saccharification, enzymatic hydrolysis was performed by the addition of commercial enzymes, Celluclast 1.5 L and Viscozyme L, after the neutralization. A maximum reducing sugar concentration of 40.4 g/L was obtained with 73% of theoretical yield from total carbohydrate. The ethanol concentration of 8.6 g/L of SHF process and 7.6 g/L of SSF process were obtained by the yeast, Saccharomyces cerevisiae KCTC 1126, with the inoculation cell density of 0.2 g dcw/L.

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Bioethanol production from wheat straw by the thermotolerant yeast Kluyveromyces marxianus CECT 10875 in a simultaneous saccharification and fermentation fed-batch process

Cited by 108 publications

References 34 publications

Cultivation of Trichosporon mycotoxinivorans in sugarcane bagasse hemicellulosic hydrolyzate

Cultivation of Trichosporon mycotoxinivorans in sugarcane bagasse hemicellulosic hydrolyzate

Study of Chemical and Enzymatic Hydrolysis of Cellulosic Material to Obtain Fermentable Sugars

Ethanol Production from Seaweed, Enteromorpha intestinalis, by Separate Hydrolysis and Fermentation (SHF) and Simultaneous Saccharification and Fermentation (SSF) with Saccharomyces cerevisiae

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