Cultivation of Trichosporon mycotoxinivorans in sugarcane bagasse hemicellulosic hydrolyzate

Abstract

Background: The yeast strain IB09 was isolated from the gut of Calosoma sp. (Carabidae, Coleoptera, Insecta) that were collected in the central Amazon rainforest. First, tolerance of the strain to ethanol and heat was tested. Then, IB09 was cultivated in a medium using sugarcane bagasse hemicellulosic hydrolyzate as a carbon source, and cell growth (OD600), specific growth rate (μMAX, h -1 ), biomass yield (YB, g.g -1 ) and relative sugar consumption (RSC, %) were evaluated. Taxonomic identification was determined by sequencing the ITS1 region of IB09 and comparing it to sequences obtained from the GenBank database (NCBI). Results: IB09 showed both ethanol tolerance and thermotolerance. Relative sugar consumption indicated that IB09 was able to perform saccharification of sugarcane bagasse hemicellulosic hydrolyzate, increasing the total reducing sugar concentration by approximately 50%. The μMAX value obtained was 0,20, indicating that cell growth was slow under the assessed conditions. Biomass yield was 0,701 g per g of consumed sugar, which is relatively high when compared with other findings in the literature. After 120 hrs of cultivation, 80,1% of total reducing sugar had been consumed. Sequencing of the ITS1 region identified IB09 as Trichosporon mycotoxinivorans. Conclusion: This is the first report to document this species in the central Amazon rainforest at this host. Trichosporon mycotoxinivorans has great biotechnological potential for use in the saccharification of sugarcane bagasse hemicellulosic hydrolyzate and for biomass production with this substrate as carbon source.

Isolation and characterization of yeasts capable of efficient utilization of hemicellulosic hydrolyzate as the carbon source

“…The addition of this yeast to broiler diets resulted in a decrease of toxins in the serum of these animal (Molnar et al, 2004;Politis et al, 2005;Hanif et al, 2011). T. mycotoxinivorans has shown great biotechnological potential in the saccharification of sugarcane bagasse hemicellulosic hydrolysate, and biomass production using this substrate as the sole carbon source (Matos et al, 2012). Eleven of the isolated species formed a group with Candida lignohabitans.…”

Isolation and Characterization of Yeasts Able to Assimilate Sugarcane Bagasse Hemicellulosic Hydrolysate and Produce Xylitol Associated with Veturius transversus (Passalidae, Coleoptera, and Insecta)

“…The beetles were washed in 70% ethanol for 1 min, the elytra were removed, and the gut was dissected. Fragments of intestine of about 1 cm were incubated for 48 h (120 rpm, 28°C) in tubes with 10 mL of SBHH, prepared as previously described [ 10 , 11 ]. After this time, 100 µ L of this suspension was spread on SBHH added to agar.…”

Scientific Opinion on the maintenance of the list of QPS biological agents intentionally added to food and feed (2012 update)

“…Among these, one report highlights the biotechnological potential of Trichosporon mycotoxinivorans for use in saccharification of hemicellulose (Matos et al, 2012) and another study documents its potential usefulness as a mycotoxin deactivator (Hanif et al, 2012 No new data certifying the lack of biological active secondary metabolites produced by this species has been retrieved and Ampelomyces quisqualis remains ineligible for a QPS recommendation.…”

“…A literature search within Pubmed and Web of Science, restricted to the last 16 months, revealed five publications concerning Trichosporon mycotoxinivorans. Among these, one report highlights the biotechnological potential of Trichosporon mycotoxinivorans for use in saccharification of hemicellulose (Matos et al, 2012) and another study documents its potential usefulness as a mycotoxin deactivator (Hanif et al, 2012). In their work aiming at evaluating the impact of Trichosporon mycotoxinivorans on liver function in mice, Khalel et al (2012) did not report any negative effects on liver enzymes.…”