Bioethanol Production from Vineyard Waste by Autohydrolysis Pretreatment and Chlorite Delignification via Simultaneous Saccharification and Fermentation

Abstract

In this paper, the production of a second-generation bioethanol from lignocellulosic vineyard cutting wastes was investigated in order to define the optimal operating conditions of the autohydrolysis pretreatment, chlorite delignification and simultaneous saccharification and fermentation (SSF). The autohydrolysis of vine-shoot wastes resulted in liquors containing mainly a mixture of monosaccharides, degradation products and spent solids (rich in cellulose and lignin), with potential utility in obtaining valuable chemicals and bioethanol. The autohydrolysis of the vine-shoot wastes was carried out at 165 and 180 °C for 10 min residence time, and the resulted solid and liquid phases composition were analysed. The resulted liquid fraction contained hemicellulosic sugars as a mixture of alpha (α) and beta (β) sugar anomers, and secondary by-products. The solid fraction was delignified using the sodium chlorite method for the separation of lignin and easier access of enzymes to the cellulosic sugars, and then, converted to ethanol by the SSF process. The maximum bioethanol production (6%) was obtained by autohydrolysis (165 °C), chlorite delignification and SSF process at 37 °C, 10% solid loading, 72 h. The principal component analysis was used to identify the main parameters that influence the chemical compositions of vine-shoot waste for different varieties.

Characterization of cardinal vine shoot waste as new resource of lignocellulosic biomass and valorization into value-added chemical using Plackett–Burman and Box Behnken

“…To the best of our knowledge, and according to the recent literature on lignocellulosic biomass from vine shoot waste, it has been used as natural resources of bioactive compounds such as production of lactic acid and lignin [26], resources of phenolic and antioxidant [27,28], cellulose nanocrystals [29], oligosaccharides [30], biobutanol [31], bioethanol [32,33] and bio-composite films [34].…”

Ethanol Production from Oil Palm Trunk: A Combined Strategy Using an Effective Pretreatment and Simultaneous Saccharification and Cofermentation

“…By this pretreatment, the complex matrix is destroyed, and the enzymes' access to the carbohydrates is facilitated. Senila et al [ 30 ] reported that the solid yields for the used autohydrolysis pretreatments were found to be between 62.2 and 71.0% (165°C) and 52.3 and 64.3% (180°C), respectively. Figure 1 shows the physical change of raw materials before and after delignification.…”

Characterization of Biobriquettes Produced from Vineyard Wastes as a Solid Biofuel Resource

“…The content of carbohydrates from the briquettes was determined as holocellulose (mixtures between cellulose and hemicelluloses). The content of holocellulose was determined according to the method presented in detail in our publication [28] as residues insoluble in NaOH. The lignin content was determined as solid weight after the reaction with H 2 SO 4 (72%).…”

“…The present paper presents a solution for producing high-quality briquettes from vine shoot waste. The results regarding vineyard pruning valorization were already published, presenting bioethanol production from cellulose extracted from vineyard waste after autohydrolysis and delignification pretreatment [28], pellets production [29], and a preliminary study of briquettes production from vine tendrils [26]. In this regard, this study aims to continue the chemical investigation, structural characterization, and combustion experiments of briquettes obtained from several Romanian vineyard varieties.…”