Bioethanol production from corn stover using aqueous ammonia pretreatment and two-phase simultaneous saccharification and fermentation (TPSSF)

Li, Xuan; Kim, Tae Hyun; Nghiem, Nhuan P.

doi:10.1016/j.biortech.2010.03.015

Cited by 123 publications

(54 citation statements)

References 15 publications

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“…Numerous studies have been published on cellulosic bioethanol production from herbaceous biomass such as corncob, cornstalks, wheat straw, and sugarcane bagasse; this is not the case with ethanol production from wood biomass such as broadleaf and coniferous trees (Mohagheghi et al 2004;Margeot et al 2009;Li et al 2010). The major sugar components of herbaceous biomass are glucose, xylose, and arabinose; those of wood biomass are glucose, xylose, and mannose.…”

Section: Discussionmentioning

confidence: 98%

Ethanol production from wood hydrolysate using genetically engineered Zymomonas mobilis

Yanase

Miyawaki

Sakurai

et al. 2012

Appl Microbiol Biotechnol

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An ethanologenic microorganism capable of fermenting all of the sugars released from lignocellulosic biomass through a saccharification process is essential for secondary bioethanol production. We therefore genetically engineered the ethanologenic bacterium Zymomonas mobilis such that it efficiently produced bioethanol from the hydrolysate of wood biomass containing glucose, mannose, and xylose as major sugar components. This was accomplished by introducing genes encoding mannose and xylose catabolic enzymes from Escherichia coli. Integration of E. coli manA into Z. mobilis chromosomal DNA conferred the ability to co-ferment mannose and glucose, producing 91 % of the theoretical yield of ethanol within 36 h. Then, by introducing a recombinant plasmid harboring the genes encoding E. coli xylA, xylB, tal, and tktA, we broadened the range of fermentable sugar substrates for Z. mobilis to include mannose and xylose as well as glucose. The resultant strain was able to ferment a mixture of 20 g/l glucose, 20 g/l mannose, and 20 g/l xylose as major sugar components of wood hydrolysate within 72 h, producing 89.8 % of the theoretical yield. The recombinant Z. mobilis also efficiently fermented actual acid hydrolysate prepared from cellulosic feedstock containing glucose, mannose, and xylose. Moreover, a reactor packed with the strain continuously produced ethanol from acid hydrolysate of wood biomass from coniferous trees for 10 days without accumulation of residual sugars. Ethanol productivity was at 10.27 g/l h at a dilution rate of 0.25 h(-1).

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Section: Discussionmentioning

confidence: 98%

Ethanol production from wood hydrolysate using genetically engineered Zymomonas mobilis

Yanase

Miyawaki

Sakurai

et al. 2012

Appl Microbiol Biotechnol

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“…14,16,24,27) Kim et al reported that in the case of SEAA treatment, the reduction in lignin content in the substrate was lower than for SAA treatment. 15,27) Simultaneous saccharification and fermentation Our results indicate that in the case of Avicel, 13.8 gL À1 of ethanol was produced, equivalent to 77.13% of the theoretical yield based on glucan levels (Table 4), while in the case of the untreated bagasse only 1.5 gL À1 of ethanol was produced, equivalent to 12.75% of the theoretical maximum yield, and the ethanol production yield for the SMAA pretreated bagasse was 11.68 gL À1 , equivalent to 90.8% of the theoretical maximum.…”

Section: )mentioning

confidence: 99%

Improvement of Hydrolysis and Fermentation of Sugarcane Bagasse by Soaking in Aqueous Ammonia and Methanolic Ammonia

Hedayatkhah

Motamedi

Varzi

et al. 2013

Bioscience, Biotechnology, and Biochemistry

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“…Akan tetapi, pengembangan bahan lignoselulosa masih terkendala oleh kelayakan ekonomi dan teknologi. Hal ini disebabkan karena struktur dan karakteristik alami lignoselulosa sangat rapat dan membutuhkan pretreatment untuk menguraikan komponen gulanya (Li et al, 2010;Alvira et al, 2010).…”

Section: Pendahuluanunclassified

Penggandaan Skala Produksi Bioetanol Dari Tongkol Jagung

Arif¹,

Diyono

Hayuningtyas

et al. 2017

Informatika Pertanian

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ABSTRAKPencarian bahan energi alternatif yang tidak berkompetisi dengan pangan dan pakan sangat perlu dan mendesak. Biomassa lignoselulosa merupakan salah satu sumber energi terbarukan yang potensial. Metode penggandaan produksi bioenergi dari skala laboratorium ke skala industri perlu dikaji untuk pengembangan. Penelitian ini bertujuan untuk mendapatkan metode penggandaan produksi bioetanol dari tongkol jagung. Percobaan produksi bioetanol dari skala laboratorium ke skala industri dilakukan dengan metode Pg/V tetap (tenaga pengadukan per volume). Perhitungan penggandaan skala berdasarkan data reologi cairan fermentasi dan spesifikasi fermentor yang digunakan. Hasil penelitian menunjukkan penggandaan skala produksi bioetanol dengan kapasitas bioreaktor 200 l, menghasilkan volume kerja 65% atau 130 liter dengan tinggi cairan fermentasi 0,840 m, diameter tangki bioreaktor 0,441 m, diameter pengaduk jenis turbin pipih 0,187 m, dan kecepatan agitasi 66,34 rpm. Berdasarkan perhitungan dasar penggandaan produksi bioetanol dengan kapasitas bioreaktor 10.000 l diperoleh volume kerja 65% atau 6.500 l dengan tinggi cairan fermentasi 2,87 m, diameter tangki bioreaktor 1,49 m, diameter pengaduk jenis turbin pipih 0,63 m, dan kecepatan agitasi 29,52 rpm.Kata kunci: jagung, tongkol, lignoselulosa, bioetanol, penggandaan produksi ABSTRACTThe effort to search for alternative energy materials that do not compete with food and feed is necessary and urgent. Lignocellulosic biomass is one potential source of renewable energy. Scalinge up methodproduction of bioenergy production from laboratory scale to industrial scale needs to be studied and developed. The aim of this study is to find get scalinge up method o0f the bioethanol production from corn cobs. An Eexperiments on scalinge up of bioethanol production from laboratory scale to industrial scale was is done by the Pg / V constant method (stirring power per volume). Scale up calculations based on data from fermented liquid rheological characteristics and specifications fermenters are used. The results showed that the calculation of basic scale up bioethanol production capacity bioreactor of 200 l, obtained working volume of 65% or 130 l, high of liquid fermentation 0.840 m, diameter tank bioreactor 0.441 m, diameter of a stirrer of turbine type of flat 0.187 m and the speed of agitation at 66.34 rpm. Based on the calculation of basic scale up bioethanol production capacity bioreactor of 10,000 l, obtained working volume of 65% amounting to 6,500 l, high of liquid fermentation 2.87 m, diameter tank bioreactor 1.49 m, diameter of a stirrer of turbine type of flat 0.63 m and the speed of agitation at 29.52 rpm.

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Bioethanol production from corn stover using aqueous ammonia pretreatment and two-phase simultaneous saccharification and fermentation (TPSSF)

Cited by 123 publications

References 15 publications

Ethanol production from wood hydrolysate using genetically engineered Zymomonas mobilis

Ethanol production from wood hydrolysate using genetically engineered Zymomonas mobilis

Improvement of Hydrolysis and Fermentation of Sugarcane Bagasse by Soaking in Aqueous Ammonia and Methanolic Ammonia

Penggandaan Skala Produksi Bioetanol Dari Tongkol Jagung

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