Bioengineered Tooth Buds Exhibit Features of Natural Tooth Buds

Smith, Elizabeth E.; Angstadt, Shantel; Monteiro, Nelson; Zhang, W.; Khademhosseini, Ali; Yelick, Pamela C.

doi:10.1177/0022034518779075

Cited by 21 publications

(12 citation statements)

References 40 publications

(49 reference statements)

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“…With the rapid advances in stem cell biology, biomaterials, biofabrication, and tissue engineering strategies, tooth regeneration seems to be possible sooner than expected (Wei et al 2013;Obregon et al 2015;Hilkens et al 2017;Lambrichts et al 2017;Smith et al 2018). TAT represents a unique biological treatment option that offers not only a solution for tooth loss in children and adolescents but also insights for future bioengineered tooth transplants.…”

Section: Discussionmentioning

confidence: 99%

Use of CBCT Guidance for Tooth Autotransplantation in Children

et al. 2019

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Tooth autotransplantation (TAT) offers a viable biological approach to tooth replacement in children and adolescents. The aim of this study was to evaluate the outcome of the cone-beam computed tomographic (CBCT)-guided TAT compared to the conventional TAT protocol and to assess the 3-dimensional (3D) patterns of healing after CBCT-guided TAT (secondary aim). This study included 100 autotransplanted teeth in 88 patients. Each experimental group consisted of 50 transplants in 44 patients (31 males and 19 females). The mean (SD) age at the time of surgery was 10.7 (1.1) y for the CBCT-guided group. This was 10.6 (1.3) y for the conventional group. The mean (SD) follow-up period was 4.5 (3.1) y (range, 1.1 to 10.4 y). Overall survival rate for the CBCT-guided TAT was 92% with a success rate of 86% compared to an 84% survival rate and a 78% success rate for the conventional group (P > 0.005). The following measurements were extracted from the 3D analysis: root hard tissue volume (RV), root length (RL), apical foramen area (AFA), and mean and maximum dentin wall thickness (DWT). Overall, the mean (SD) percentage of tissue change was as follows: RV gain by 65.8% (34.6%), RL gain by 37.3% (31.5%), AFA reduction by 91.1% (14.9%), mean DWT increase by 107.9% (67.7%), and maximum DWT increase by 26.5% (40.1%). Principal component analysis (PCA) identified the mean DWT, RV, and maximum DWT as the parameters best describing the tissue change after TAT. Cluster analysis applied to the variables chosen by the PCA classified the CBCT group into 4 distinct clusters (C1 = 37.2%, C2 = 17.1%, C3 = 28.6%, C4 = 17.1%), revealing different patterns of tissue healing after TAT. The CBCT-guided approach increased the predictability of the treatment. The 3D analysis provided insights into the patterns of healing. CBCT-guided TAT could be adopted as an alternative for the conventional approach. (Clinical trial center and ethical board University Hospitals, KU Leuven: S55287; ClinicalTrials.gov Identifier: NCT02464202

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Section: Discussionmentioning

confidence: 99%

Use of CBCT Guidance for Tooth Autotransplantation in Children

et al. 2019

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“…These observations indicate the self-organizing ability of 3-dimensional DPSCs constructs within the pulpless root canal in vivo and that pulplike tissue, rich in blood vessel, can be formed with DPSCs without the use of scaffolds or growth factors [53]. More recently, Smith et al [54] reported on their work to create highly cellularized bioengineered tooth bud constructs that would develop hallmark features of natural tooth buds such as the dental epithelial stem cell niche, enamel knot signaling centers, transient amplifying cells, and mineralized dental tissues. These constructs consisted of postnatal dental cells which were encapsulated within gelatin methacryloyl hydrogel and were implanted under the skin of immunocompromised rats which demonstrated evidence of natural tooth development.…”

Section: Scaffolds and Growth Factors For Regenerative Dentistrymentioning

confidence: 85%

Stem cells and tooth regeneration: prospects for personalized dentistry

et al. 2019

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Over the last several decades, a wealth of information has become available regarding various sources of stem cells and their potential use for regenerative purposes. Given the intense debate regarding embryonic stem cells, much of the focus has centered around application of adult stem cells for regenerative engineering along with other relevant aspects including use of growth factors and scaffolding materials. The more recent discovery of tooth-derived stem cells has sparked much interest in their application to regenerative dentistry to treat and alleviate the most prevalent oral diseases-i.e., dental caries and periodontal diseases. Also exciting is the advent of induced pluripotent stem cells, which provides the means of using patient-derived somatic cells for their creation, and their eventual application for generation of the dental complex. Thus, evolving developments in the field of regenerative dentistry indicate the prospect of constructing Bcustom-made^tooth and supporting structures thereby fostering the realization of Bpersonalized dentistry.^On the other hand, others have explored the possibility of augmenting endogenous regenerative capacity through utilization of small molecules to regulate molecular signaling mechanisms that mediate regeneration of tooth structure. This review is focused on these aspects of regenerative dentistry in view of their relevance to personalized dentistry.

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“…As regards the scaffold materials used for the tissue engineering process of the whole tooth, one can mention hydrogels based on gelatin methacrylate [ 227 , 228 , 229 ], gelatin methacrylamide [ 230 ] and methacryloyl [ 231 ], polyglycolate [ 232 ], and poly (D,Llactide-co-glycolide) in combination with gelatin [ 233 ], silk [ 234 ], and decellularized scaffolds [ 235 , 236 ]. In these research studies, the cultured dissociated porcine and dental mesenchymal cells were combined, seeded on different biodegradable scaffolds, i.e., polyglycolate and poly-L-lactate-coglycolate or polyglycolate–poly-L-lactate transplanted and grown in rat hosts.…”

Section: Whole Tooth Engineeringmentioning

confidence: 99%

“…Smith et al [ 231 ] reported the obtaining of vastly cellularized dental buds which exhibited distinctive features common to those of natural ones, such as enamel knot signaling centers, niche of dental epithelial stem cells, mineralized dental tissues, and transient amplification cells. The tooth development was demonstrated by using postnatal dental cells encapsulated inside gelatin methacryloyl hydrogel scaffolds and further implanted beneath the skin of some immunocompromised rats.…”

Section: Whole Tooth Engineeringmentioning

confidence: 99%

Hard Dental Tissues Regeneration—Approaches and Challenges

Olaru¹,

Sachelarie

Calin

2021

Materials

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With the development of the modern concept of tissue engineering approach and the discovery of the potential of stem cells in dentistry, the regeneration of hard dental tissues has become a reality and a priority of modern dentistry. The present review reports the recent advances on stem-cell based regeneration strategies for hard dental tissues and analyze the feasibility of stem cells and of growth factors in scaffolds-based or scaffold-free approaches in inducing the regeneration of either the whole tooth or only of its component structures.

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Bioengineered Tooth Buds Exhibit Features of Natural Tooth Buds

Cited by 21 publications

References 40 publications

Use of CBCT Guidance for Tooth Autotransplantation in Children

Use of CBCT Guidance for Tooth Autotransplantation in Children

Stem cells and tooth regeneration: prospects for personalized dentistry

Hard Dental Tissues Regeneration—Approaches and Challenges

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