Bioenergetics of rat prostate cancer cell migration

Kouvroukoglou, S.; Lakkis, Clair L.; Wallace, James D.; Zygourakis, Kyriacos; Epner, Daniel E.

doi:10.1002/(sici)1097-0045(19980201)34:2<137::aid-pros9>3.0.co;2-h

Cited by 11 publications

(10 citation statements)

References 33 publications

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“…The literature similarly shows that the MatLu prostate tumour cells grown in glucose-free medium presented a lower migration rate than those cells treated with 11.1 mmol/L of glucose (Kouvroukoglou et al, 1998). Our results showed that PC3 cells conditioned to HF and HG media presented accelerated migration evidenced by the cell scratch assay when compared to the C group.…”

Section: Discussionsupporting

confidence: 71%

“…Our results showed that PC3 cells conditioned to HF and HG media presented accelerated migration evidenced by the cell scratch assay when compared to the C group. The literature similarly shows that the MatLu prostate tumour cells grown in glucose-free medium presented a lower migration rate than those cells treated with 11.1 mmol/L of glucose (Kouvroukoglou et al, 1998). Regarding fatty acids, Pillon et al (2015), using the endothelial cells HAMEC associated with THP-1 monocytes, have shown that the treatment of these cells with 200 mM palmitate increased the expression of adhesion molecules by endothelial cells, stimulating monocyte transmigration.…”

Section: Discussionmentioning

confidence: 94%

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Effect of glucose and palmitate environment on proliferation and migration of PC3‐prostate cancer cells

Rezende

Galheigo

Landim

et al. 2019

Cell Biology International

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Recent studies have been trying to find out how diet and metabolic changes such as dyslipidaemia, hyperglycaemia, and hyperinsulinaemia can stimulate cancer progression. This investigation aimed to evaluate the effect of high concentrations of fatty acids and/or glucose in tumour prostate cells, focusing on the proliferation/migration profile and oxidative stress. PC3 cells were treated with high concentration of saturated fatty acid (palmitate, 100 mM), glucose (220 mg/dL), or both for 24 or 48 h. Results demonstrated that PC3 cells showed a significant increase in proliferation after 48 h of treatment with glucose and palmitateþglucose. Cell proliferation was associated with reduced levels of AMPK phosphorylation in glucose group at 24 and 48 h of treatment, while palmitate group presented this result only after 48 h of treatment. Also, there was a significant increase in cell migration between time 0 and 48 h after all treatments, except in the control. Catalase activity was increased by palmitate in the beginning of treatment, while glucose presented a later effect. Also, nitrite production was increased by glucose only after 48 h, and the total antioxidant activity was enhanced by palmitate in the initial hours. Thus, we conclude that the high concentration of the saturated fatty acid palmitate and glucose in vitro influences PC3 cells and stimulates cellular activities related to carcinogenesis such as cell proliferation, migration, and oxidative stress in different ways. Palmitate presents a rapid and initial effect, while a glucose environment stimulates cells later on, maintaining high levels of cell proliferation.

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Section: Discussionsupporting

confidence: 71%

Section: Discussionmentioning

confidence: 94%

Effect of glucose and palmitate environment on proliferation and migration of PC3‐prostate cancer cells

Rezende

Galheigo

Landim

et al. 2019

Cell Biology International

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“…Similar results were observed by Kouvroukoglou et al . (12), who showed that inhibition of glycolysis or electron transport did not significantly affect intracellular ATP levels or motility of MatLyLu cells.…”

Section: Discussionmentioning

confidence: 99%

“…Real‐time changes in oxygen tension and metabolism were measured on‐line for two cancer cell lines, MCF‐7 and MatLyLu. Oxygen consumption was modulated using a well‐known uncoupler of oxidative phosphorylation, the protonophore carbonyl cyanide m‐chlorophenylhydrazone (CCCP), and antimycin, an inhibitor of oxidative phosphorylation (10–12).…”

mentioning

confidence: 99%

Real‐time measurements of cellular oxygen consumption, pH, and energy metabolism using nuclear magnetic resonance spectroscopy

Pilatus

Aboagye

Artemov

et al. 2001

Magnetic Resonance in Med

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Changes in molecular expression or apoptotic behavior, induced by malignant transformation or anticancer treatment, are frequently reflected in cellular metabolism and oxygen consumption. A technique to monitor oxygen consumption, cell physiology, and metabolism noninvasively would provide a better understanding of interactions between molecular changes and metabolism in malignant transformation and following cancer treatment. Such a system was developed in this study by adapting multinuclear MRI and spectroscopic techniques to an isolated cell perfusion system. The system was evaluated by studying the effects of two agents, carbonyl cyanide m-chlorophenylhydrazone ( The two major energy-providing metabolic pathways, oxidative phosphorylation and glycolysis, play an important role in malignant transformation and in the action of anticancer agents and radiation therapy. Overexpression of certain oncogenes, such as ras and myc, can effect changes in mitochondrial membrane potential, cellular oxygen consumption, and glycolytic/oxidative metabolism (1-4). Modulation of oxygen-dependent processes in the cell can also enhance the cytotoxic effects of anticancer drugs, as was demonstrated for lonidamine, an inhibitor of electron transport and oxygen consumption, and doxorubicin (5,6). To understand the impact of oxygen on cancer cell physiology and metabolism, and the action of certain anticancer agents, it is necessary to measure metabolic parameters such as oxygen consumption, lactate production, pH changes, ATP production, and glucose utilization, either interleaved or simultaneously, over the same period of time. Currently no single method exists to obtain such on-line measurements in real time. Nuclear magnetic resonance (NMR) spectroscopy can be used to measure pH, ATP levels, intracellular lactate levels, and glycolytic flux.Polarographic measurements of oxygen tension in the influx and efflux perfusate have been used in NMR cell perfusion systems to determine oxygen consumption (7,8). These measurements, however, are obtained at sampling points distant from the cell sample, and oxygen permeation through the perfusion lines may cause errors-particularly at low perfusion rates.McGovern et al. (9) originally proposed using 19 F NMR to measure oxygen concentrations, from T 1 relaxation rates of perfluorocarbons (PFC) embedded into microcarriers used for cell immobilization. In their study, cellular oxygen consumption rates were derived from complex calculations of the volume-averaged data. Subsequently, we envisaged that spatially localized PFC T 1 relaxation rates, measured in cell-free PFC-doped alginate beads positioned at the bottom and top of the active volume of the NMR sample tube, would directly provide oxygen consumption values for a cell sample encompassed by the two layers. In this work we report the design and evaluation of such a system, which is capable of measuring oxygen consumption together with ATP levels and pH of immobilized perfused cells. Lactate levels in medium collected from the influx and ...

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“…We used dialyzed serum for experiments involving D-glucose deprivation, since dialyzed serum contains only about 1% of the physiologic D-glucose concentration. Tumor cells deprived of glucose in the current studies were not completely starved of nutrients, but instead relied upon mitochondrial metabolism of amino acids for ATP production (Pedersen, 1978;Kouvroukoglou et al, 1998).…”

Section: Effect Of Glucose On Proliferation Of Prostate Cancer Cellsmentioning

confidence: 99%

Regulation of prostate cancer cell division by glucose

et al. 1999

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Previous studies have shown that rapid cell proliferation is associated with elevated glucose consumption. However, those studies did not establish whether glucose is required for prostate cancer cell proliferation or define the molecular mechanisms by which glucose regulates cell division. We addressed these issues by studying two metastatic human prostate cancer cell lines: DU145, which is androgen independent and highly proliferative; and LNCaP, which is androgen dependent and relatively slow growing. We found that proliferation of DU145 cells was significantly inhibited by reduction of glucose in the medium to 0.5 g/L, which is half the physiologic concentration, whereas LNCaP cells grew at control rates even in the presence of only 0.05 g/L glucose. Glucose deprivation of DU145 cells caused a 90% reduction in DNA synthesis; a 10-20-fold reduction in cyclins D and E and CDK4 levels; and cell cycle arrest in G0-G1. However, glucose deprivation did not cause global inhibition of protein synthesis, since mutant p53 levels increased in glucose-deprived DU145 cells. This observed increase in mutant p53 levels was not associated with a rise in p21 levels. Glucose deprivation of DU145 cells also led to apparent dephosphorylation of mutant retinoblastoma (RB) protein. We conclude that: 1) high levels of glucose consumption are required for rapid proliferation of androgen-independent prostate cancer cells, 2) glucose may not be required for slow growth of androgen-dependent prostate cancer cells, and 3) glucose promotes passage of cells through early G1 by increasing the expression of several key cell cycle regulatory proteins that normally inhibit RB function.

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Bioenergetics of rat prostate cancer cell migration

Cited by 11 publications

References 33 publications

Effect of glucose and palmitate environment on proliferation and migration of PC3‐prostate cancer cells

Effect of glucose and palmitate environment on proliferation and migration of PC3‐prostate cancer cells

Real‐time measurements of cellular oxygen consumption, pH, and energy metabolism using nuclear magnetic resonance spectroscopy

Regulation of prostate cancer cell division by glucose

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