Bioelectrochemical Systems as Technologies for Studying Drug Interactions Related to Cytochrome P450

Shumyantseva, Victoria V.; Makhova, A.A.; Ших, Е. В.; Булко, Т. В.; Kuzikov, Alexey V.; Masamrekh, Rami A.; Shkel, Tatsiana; Usanov, Sergey A.; Gilep, Andrei A.; Archakov, Alexander I.

doi:10.1007/s12668-018-0567-7

Cited by 13 publications

(3 citation statements)

References 38 publications

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“…118 Immobilization of enzymes on working electrodes is an important tool to produce or generate the drug metabolites and other products with high yield and high selectivity for a fast toxicity screening. 116,[119][120][121][122][123][124][125][126][127] Electrochemical techniques can also scale up the generation of metabolites in sufficient amounts for investigation of metabolite or structural characterization. However, activation has often been driven by the H 2 O 2 shunt, and not by the accepted biological pathway in Scheme 2.…”

Section: Rationales For Electrochemical Activation Of Heme Enzymesmentioning

confidence: 99%

Electrochemical transformations catalyzed by cytochrome P450s and peroxidases

Kumar

Rusling

2023

Chem. Soc. Rev.

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Section: Rationales For Electrochemical Activation Of Heme Enzymesmentioning

confidence: 99%

Electrochemical transformations catalyzed by cytochrome P450s and peroxidases

Kumar

Rusling

2023

Chem. Soc. Rev.

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“…Ðàíåå áûëî èññëåäîâàíî âëèÿíèå âèòàìèíîâ-àíòèîêñèäàíòîâ íà àêòèâíîñòü öèòîõðîìà Ð450 3À4 ìåòîäîì ýëåêòðîàíàëèçà [19,20]. Òàóðèí (2-àìèíîýòàíñóëüôîíîâàÿ êèñëîòà) ÿâëÿåòñÿ íàèáîëåå ðàñïðîñòðàíåííîé èç ñâîáîäíûõ àìèíîêèñëîò â îðãàíèçìå ÷åëîâåêà, âëèÿþùåé íà ñîñòîÿíèå êëåòî÷íîãî èììóíèòåòà, óðîâåíü àíòèîêñèäàíòíîé çàùèòû, äåòîêñèêàöèîííûõ âîçìîaeíîñòåé îðãàíèçìà.…”

Section: ââåäåíèåunclassified

Влияние Таурина И L-Карнитина На Активность Цитохрома P450 3A4 У Добровольцев

Makhova¹,

Ших²,

Булко³

et al. 2020

Эксп. и клин. фармакол.

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Проведено сравнительное исследование соотношения 6β-гидроксикортизол/кортизол в моче у добровольцев с целью выяснения влияния таурина и L-карнитина на активность цитохрома P450 3A4. Выявлено, что соотношение 6β-гидроксикортизол/кортизол в утренней порции мочи добровольцев до начала применения таурина составило (2,7 ± 0,2). Через 3 дня введения внутрь таурина (500 мг 2 раза в сутки) соотношение 6β-гидроксикортизол/кортизол в утренней порции мочи было (3,02 ± 0,2), что свидетельствует о стимулирующем действии таурина на каталитическую активность цитохрома P450 3A4 (p < 0,05). Результаты оценки соотношения 6β-гидроксикортизол/кортизол в моче y добровольцев на фоне приема L-карнитина (750 мг 3 раза в день внутрь) в течение 14 дней не показали каких-либо значимых изменений. Результаты, полученные при исследовании y добровольцев, подтверждают отсутствие влияния L-карнитина на активность цитохрома P450 3A4. Полученные результаты позволяют прогнозировать отсутствие межлекарственного взаимодействия на уровне цитохрома P450 3A4 при применении L-карнитина в составе комбинированной терапии одновременно c другими лекарственными средствами, субстратами этого фермента.

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“…While biochemical in vitro assays and methods are valuable, an alternate complementing analytical technique is to drive the purified P450 and microsomal biocatalysis electrochemically under an applied reductive potential with simplicity, creating a bioelectrocatalytic system. , In this system, the P450 enzyme is attached to an electrode surface either in the purified form or bound to the microsomal membrane or other support materials. − When a substrate is added to the system, the P450 enzyme catalyzes the oxidation of the substrate, generating an electrochemical signal that can be measured. In the same system, in the place of substrates, specific P450 inhibitors can be used to profile the activity of a specific P450 isoform toward a drug conversion into metabolites …”

Section: Introductionmentioning

confidence: 99%

Nanobioelectrocatalysis Using Human Liver Microsomes and Cytochrome P450 Bactosomes: Pyrenyl-Nanocarbon Electrodes

Premaratne,

Niroula,

Moulton

et al. 2024

ACS Appl. Bio Mater.

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Human liver microsomes containing various drugmetabolizing cytochrome P450 (P450) enzymes, along with their NADPH-reductase bound to phospholipid membranes, were absorbed onto 1-pyrene butylamine pi−pi stacked with aminefunctionalized multiwalled carbon nanotube-modified graphite electrodes. The interfaced microsomal biofilm demonstrated direct electrochemical communication with the underlying electrode surface and enhanced oxygen reduction electrocatalytic activity typical of heme enzymes such as P450s over the unmodified electrodes and nonenzymatic currents. Similar enhancements in currents were observed when the bioelectrodes were constructed with recombinant P450 2C9 (single isoform) expressed bactosomes. The designed liver microsomal and 2C9 bactosomal bioelectrodes successfully facilitated the electrocatalytic conversion of diclofenac, a drug candidate, into 4′-hydroxydiclofenac. The enzymatic electrocatalytic metabolite yield was several-fold greater on the modified electrodes than on the unmodified bulk graphite electrodes adsorbed with a microsomal or bactosomal film. The nonenzymatic metabolite production was less than the enzymatically catalyzed metabolite yield in the designed microsomal and bactosomal biofilm electrodes. To test the throughput potential of the designed biofilms, eight-electrode array configurations were tested with the microsomal and bactosomal biofilms toward electrochemical 4′-hydroxydiclofenac metabolite production from diclofenac. The stability of the designed microsomal bioelectrode was assessed using nonfaradaic impedance spectroscopy over 40 h, which indicated good stability.

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Bioelectrochemical Systems as Technologies for Studying Drug Interactions Related to Cytochrome P450

Cited by 13 publications

References 38 publications

Electrochemical transformations catalyzed by cytochrome P450s and peroxidases

Electrochemical transformations catalyzed by cytochrome P450s and peroxidases

Влияние Таурина И L-Карнитина На Активность Цитохрома P450 3A4 У Добровольцев

Nanobioelectrocatalysis Using Human Liver Microsomes and Cytochrome P450 Bactosomes: Pyrenyl-Nanocarbon Electrodes

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