Bioelectrochemical immunoassay for human chorionic gonadotrophin in serum using an electrode-immobilised capture antibody

Robinson, G.A.; Cole, V.M.; Rattle, S J; Forrest, G C

doi:10.1016/0265-928x(86)85012-x

Cited by 41 publications

(14 citation statements)

References 17 publications

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“…These authors report a quantitative assay in the range 10 4 to 10 7 cfu/ml. Recently, some reports have described the use of an electrode surface as both the immunological solid phase and as the electrochemical detector (17)(18)(19). In a previous publication we have described preliminary results obtained with an immunsensor based upon the enzymatic reaction of alkaline phosphatase with p-aminophenyl phosphate (20).…”

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confidence: 98%

“…In that system, glassy carbon served both as the solid phase in the immunorecognition reaction and as an amperometric electrode for oxidation of p-aminophenol formed by the enzymatic reaction. Glassy carbon electrodes were also used in a heterogeneous immunoassay using glucose oxidase as an enzyme label (17)(18). By using a rotating disc electrode, Huet et al (21) have recently shown that mass transfer to the solid phase, consisiting of antibodies immobilized on the glassy carbon, is a key step in heterogeneous immunassay.…”

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confidence: 99%

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Immunoelectrodes for the Detection of Bacteria

Rishpon

Gezundhajt

Soussan

et al. 1992

ACS Symposium Series

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This work describes a rapid and sensitive method for the determination of bacteria. The method is based on an enzyme-taggedimmuno-electrochemical assay. Antibodies are immobilized on disposable carbon felt disc electrodes and are used to capture antigens in test solutions. After a short incubation with a second antibody, which is labeled with the enzyme alkaline phosphatase, the activity of the enzyme electrode thus formed is measured. This enzyme reacts with the substrate p-aminophenyl phosphate and the product of this enzymatic reaction, p-aminophenol, is detected amperometrically. The use of rotating electrodes significantly shortens the incubation times and, together with the computerized electrochemical system, results in extremely high sensitivity. The results obtained with Staphylococcus aereus and Escherichia coli cells show that the system can detect as low as 10 cell/ml in less than 10 minutes.There is a great need for fast and reliable devices capable of detection and identification of various types of bacteria. Traditional microbiological methods are based on cultural techniques which are tedious and slow. A considerable effort has been invested in development of detection methods based on immunological interactions. These methods, which generally involve labeling of antibodies with radioactive, fluorescent or enzymatic label, have been shown to be quite effective, giving specific and quantitative detection of target antigens. During the last decade several attempts were made to combine the specificity of antibody-antigen interaction with the high sensitivity, wide dynamic range, and simplicity of electroanalytical methods. These attempts led to the development of electrochemical immunosensors, several of which detect the corresponding antigen at extremely high sensitivity (1-5).

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confidence: 98%

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confidence: 99%

Immunoelectrodes for the Detection of Bacteria

Rishpon

Gezundhajt

Soussan

et al. 1992

ACS Symposium Series

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“…This has been demonstrated by Robinson et al in their studies on electrochemical immunoassays for hCG 15 and thyroxine. 16 In the analysis of hCG, a "twosite" amperometric immunoassay was developed in which monoclona.l "capture" antiborlies were immobilised on the surface of a glassy carbon electrode.…”

Section: Mediation Of Response By Electron Transfer Reagentmentioning

confidence: 71%

“…This method was found to correlate weil with an imrnunoradiometric a.ssay. 15 In the analysis of thyroxine, another ferrocene derivative, namely ferrocenemonocarboxylic acid, was used as the electron transfer mediator. 16 …”

Section: Mediation Of Response By Electron Transfer Reagentmentioning

confidence: 99%

Direct Electrochemical Immunoassays Involving Adsorbed or Immobilised Species

Smyth¹,

Buckley²,

Rodríguez

et al. 1990

Contemporary Electroanalytical Chemistry

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“…High-performance immunoaffinity chromatography (HPIC), for example, has shown a good sensitivity with an analyte detection limit around 10"12 mol L"1 (9). In these techniques, the site of immunoreaction and the site of electrochemical detection are spatially separated, but recently, Robinson et al (10) have refined this scheme by covalent immobilization of the capture antibody on the electrode surface itself. We think that this method is very promising and our aim in this paper is to extend both theoretically and experimentally this principle.…”

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confidence: 99%