Bioefficacy of Tea Catechins Associated with Milk Caseins Tested Using Different In Vitro Digestion Models

Guri, Anilda; Haratifar, Sanaz; Corredig, Milena

doi:10.1007/s13228-014-0035-y

Cited by 25 publications

(29 citation statements)

References 55 publications

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“…The anti-proliferation activity of curcumin encapsulated in SSPS before and after treatment in simulated gastric and intestinal digestive fluids was compared to free curcumin dissolved in dimethyl sulfoxide (DMSO). The simulated gastric and intestinal digestion conditions followed literature methods, with slight modification (Guri, Haratifar, & Corredig, 2014; Luo, Pan, & Zhong, 2015). Pepsin and pancreatin were dispersed separately at 8.0 mg/ml and 80.0 mg/ml in 10 mM phosphate-buffered saline adjusted to pH 2.0 and pH 7.0, respectively.…”

Section: Methodsmentioning

confidence: 99%

Self-assembled curcumin-soluble soybean polysaccharide nanoparticles: Physicochemical properties and in vitro anti-proliferation activity against cancer cells

et al. 2018

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Nanoencapsulation of lipophilic bioactive compounds in food biopolymers is important to functional beverages, but protein-based nanocapsules are unstable around the isoelectric point of protein. The objectives of this work were to study physicochemical properties of self-assembled curcumin-soluble soybean polysaccharide (SSPS) nanoparticles and evaluate the activities against proliferation of human colon HCT116 and mammary adenocarcinoma MCF-7 cancer cells before and after simulated digestions. Capsules with a hydrodynamic diameter of 200-300 nm and an encapsulation efficiency of ∼90% were self-assembled after increasing curcumin-SSPS mixture to pH 12.0 and lowering pH to 7.0. The capsule dispersions were stable at pH 2.0-7.0 and after heating at 95 °C for 1 min. No significant difference was observed for the viability of HCT 116 and MCF-7 cells challenged with 0.4, 4.0, and 40 μg/ml nanoencapsulated curcumin before and after simulated gastric and intestinal digestions. These findings may be significant to help develop functional beverages for disease prevention.

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Section: Methodsmentioning

confidence: 99%

Self-assembled curcumin-soluble soybean polysaccharide nanoparticles: Physicochemical properties and in vitro anti-proliferation activity against cancer cells

et al. 2018

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“…In our study, catechins were selectively incorporated into rice bran, which provides a promising way to enrich catechins on food matrix directly from caffeine‐containing crude tea extract. Yet, the selectivity of food matrix for carrying catechins has not been fully discussed, since most studies investigated the incorporation with neat catechins or individual catechin (Dube and others ; Shpigelman and others ; Guri and others ; Wu and others ,b). Defatting pretreatment enhanced the adsorption of tea catechins on rice bran, which is possibly attributed to a relative increased content of key components responsible for catechins adsorption, as lipids were removed.…”

Section: Resultsmentioning

confidence: 99%

Using Defatted Rice Bran as a Bioadsorbent for Carrying Tea Catechins

Shi

Yang

Jin³

et al. 2015

Journal of Food Science

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Rice bran is regarded as a good fibre source that can be added to various food products and health supplements, which is a potential biocarrier for bioactives. Our study showed that defatted rice bran had a high affinity for tea catechins but caffeine, and provided a promising way for selective enrichment of catechins on defatted rice bran under practical condition. Protein and cellulose are the main contributors to the adsorption of tea catechins on defatted rice bran.

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“…These cells are able to create a tight monolayer with well-developed tight junctions once they reach full confluency, as a close representation of the epithelial layer in the human gut 22 . We have been successfully using this cell model either in isolate or in combination with HT-29-MTX cells as a valid approach to access the uptake of bioactives in the small intestine 4, 5,23 . DMEM supplied with 10 FBS, 1 antibiotic solution 10,000 units/mL of penicillin and 10,000 μg/mL of streptomycin , 1 NAA and 0.25 HEPES, was used for propagation of the cells.…”

Section: Cell Culturementioning

confidence: 99%

Delivery of Curcumin Using Skim Milk or Oil in Water Emulsions: Effect of the Matrices on Cellular Uptake

et al. 2018

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To enhance the curcumin delivery in a variety of food grade matrices namely spray dried ethanolic curcumin in fresh skim milk (Spray dried Cu-SM), a fresh mixture of ethanolic curcumin and skim milk (Fresh Cu-SM) a powder mixture of curcumin and skim milk powder (Powder Cu-SMP) and oil in water emulsion (Emulsion) were studied. The cellular uptake of curcumin from the respective matrices was studied on Caco-2 cell monolayers. Spray dried Cu-SM showed higher encapsulation efficiency compared to a corresponding Powder Cu-SMP and an oil-in-water emulsion (40% oil) bearing curcumin. Furthermore, ethanolic administration of curcumin in spray dried form enhanced the cellular uptake of curcumin considerably higher than non-ethanolic samples (approx. 4 times). Overall, milk protein based vectors were found to perform better than emulsion samples. These findings highlighted the fact that curcumin uptake may be tailored by fine tuning of curcumin delivery vehicles which highlights possible application of powders as functional foods.

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Bioefficacy of Tea Catechins Associated with Milk Caseins Tested Using Different In Vitro Digestion Models

Cited by 25 publications

References 55 publications

Self-assembled curcumin-soluble soybean polysaccharide nanoparticles: Physicochemical properties and in vitro anti-proliferation activity against cancer cells

Self-assembled curcumin-soluble soybean polysaccharide nanoparticles: Physicochemical properties and in vitro anti-proliferation activity against cancer cells

Using Defatted Rice Bran as a Bioadsorbent for Carrying Tea Catechins

Delivery of Curcumin Using Skim Milk or Oil in Water Emulsions: Effect of the Matrices on Cellular Uptake

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