Self-assembled curcumin-soluble soybean polysaccharide nanoparticles: Physicochemical properties and in vitro anti-proliferation activity against cancer cells

Abstract

Nanoencapsulation of lipophilic bioactive compounds in food biopolymers is important to functional beverages, but protein-based nanocapsules are unstable around the isoelectric point of protein. The objectives of this work were to study physicochemical properties of self-assembled curcumin-soluble soybean polysaccharide (SSPS) nanoparticles and evaluate the activities against proliferation of human colon HCT116 and mammary adenocarcinoma MCF-7 cancer cells before and after simulated digestions. Capsules with a hydrodynamic diameter of 200-300 nm and an encapsulation efficiency of ∼90% were self-assembled after increasing curcumin-SSPS mixture to pH 12.0 and lowering pH to 7.0. The capsule dispersions were stable at pH 2.0-7.0 and after heating at 95 °C for 1 min. No significant difference was observed for the viability of HCT 116 and MCF-7 cells challenged with 0.4, 4.0, and 40 μg/ml nanoencapsulated curcumin before and after simulated gastric and intestinal digestions. These findings may be significant to help develop functional beverages for disease prevention.

Complexation of curcumin with whey protein isolate for enhancing its aqueous solubility through a solvent‐free pH‐driven approach

“…Curcumin‐WPI complexes were prepared by pH shifting method according to Pan, Chen, Baek, and Zhong () with slight modifications. WPI powder was dispersed at a concentration of 20 mg/ml in distilled water and were continuously stirred for 2 hr at room temperature and then they were fully hydrated overnight at 4°C.…”

Therapeutic effects of curcumin on sepsis and mechanisms of action: A systematic review of preclinical studies

“…Curcumin is widely used in medicine due to medicinal properties, cost-effectiveness, and simple extraction from a turmeric plant that grows in different regions in the world. Recent evidences have shown that curcumin possesses multiple biological activities and pharmacological properties including anti-inflammation (Dhir, 2018), antioxidation (Khanji et al, 2018), anticancer and antiproliferation (Barati, Momtazi-Borojeni, Majeed, & Sahebkar, 2019;Pan, Chen, Baek, & Zhong, 2018), antimicrobial (Marini et al, 2018), and free radical scavenger (Vaughn et al, 2017;Zhong et al, 2016). Some studies have suggested protective effects of curcumin against numerous diseases such as metabolic syndrome (due to improving insulin sensitivity, suppressing adipogenesis, reducing elevated blood pressure and decrease inflammation) (Ghazimoradi et al, 2017), inflammatory bowel disease (inhibit nuclear factor kappa-light-chain-enhancer of activated B [NF-κB], 5-lipoxygenase, and Toll-like receptor 4 (TLR-4)) (Singla et al, 2014), rheumatoid arthritis (due to regulating immune response and decrease the expression of cytokines and chemokine, (such as monocyte chemoattractant protein1 (MCP-1)) (Khayyal et al, 2018), cardiovascular disease (due to adjustment of the enzyme related to the metabolism of lipoproteins, decrease inflammation, and oxidative stress) (Li et al, 2019), and sepsis (due to modulate immune responses) (Chen, Lu, et al, 2018).…”

Peptide-Carbon Quantum Dots conjugate, Derived from Human Retinoic Acid Receptor Responder Protein 2, against Antibiotic-Resistant Gram Positive and Gram Negative Pathogenic Bacteria

“…Xiaohui Gao and co-workers reported that shifts in the emission peak contribute to the strong interaction between water molecules and excited dipole moments in CQDs in solution [36]. Shifting of peaks confirm the structural change in HSER due to interaction with CQDs [37]. The dialyzed HSER CQDs were further characterized using HPLC coupled with ESI-qTOF-MS to obtain the individual peaks for HSER-CQD components, but, due to the small size of CQDs, they were not spotted in HPLC; however, sharp peaks for the presence of HSER were obtained, as shown in the Figure 2C.…”