Biodiesel synthesis from microalgae using immobilized Aspergillus niger whole cell lipase biocatalyst

Abstract

a b s t r a c tWhole cell lipase catalysis and microalgal feedstocks make overall biodiesel synthesis greener and sustainable. In this study, a novel approach of whole cell lipase-catalyzed conversion of Scenedesmus obliquus lipids was investigated for biodiesel synthesis. Microalgal biodiesel was characterized for its fuel properties. Optimization of process parameters for immobilized Aspergillus niger whole cell lipasecatalyzed biodiesel synthesis was carried out. Highest biodiesel conversion of 53.76% was achieved from S. obliquus lipids at 35 C, methanol to oil ratio of 5:1 and 2.5% water content based on oil weight with 6 BSPs (Biomass support particles).Step-wise methanol addition was applied to account for methanol tolerance, which improved biodiesel conversion upto 80.97% and gave 90.82 ± 1.43% yield. Immobilized A. niger lipase can be used for 2 batches without significant loss in conversion efficiency. Most of the fuel properties of biodiesel met the specifications set by international standards.

Optimization of Biodiesel Production from Waste Cooking Oil Using Waste Eggshell as a Base Catalyst under a Microwave Heating System

“…Figure 2 shows the absorption bands of unburnt and incinerated eggshell, with incineration occurring at 1000 • C for two hours. The pre-incineration absorption bands centered at 1421, 875 and 713 cm −1 , and this spectrum is due to the presence of carbonate, CO 2− 3 , species on the catalyst surface [4,26,34,35]. Meanwhile, the post-incineration absorption bands centered at 3643, 1415, 1049, 524 and 430 cm −1 .…”

“…At present, many states in the USA are advocating for an expansion of the production and application of biodiesel, whereas the US central administration has adopted a credit tax system as a great motivation to enhance this initiative [2]. Several studies have discovered the discharges of polycyclic aromatic hydrocarbons (PAHs), hydrocarbons (HCs), polychlorinated dibenzo-p-dioxin/dibenzofurans, carbon monoxide (CO), and sulfur dioxide (SO 2 ), among others, can be significantly lowered whenever diesel engines use biodiesel as an unconventional fuel [3][4][5][6][7][8][9][10][11][12][13][14][15].…”

Biodiesel production using magnetic whole-cell biocatalysts by immobilization of Pseudomonas mendocina on Fe3O4-chitosan microspheres

“…3, the biodiesel yield was improved significantly with the highest biodiesel yield of 90.48% obtained after 72 h. However, the fifth addition of methanol only gives an increase of 3.13% in biodiesel yield. The possible reason for this variation is that excess addition of methanol could result in a lower concentration of lipids, which leads to a negative effect to enzymatic activity [29]. These results reveal that the deactivation of MWCBs is avoided by the stepwise addition of methanol.…”

ENZYMATIC GLYCEROLYSIS OF METHYL LAURATE UTILIZING Candida antarctica Lipase b

“…The physiological role of lipases (water-soluble triacylglycerol acylhydrolases) is the catalytic conversion of tri-glycerides into di-or mono-glycerides, fatty acids and glycerol [9]. Extracellular lipases from Candida sp., Pseudomonas sp., Thermomyces lanuginosus and Rhizomucor miehei have been investigated by a considerable number of studies to be employed in the biodiesel synthesis [10]. Lipases are hydrolases that catalyze the hydrolysis of fatty acid esters found in living organisms [11].…”