Enzymatic glycerolysis using Candida antarctica lipase b (CALB) as catalyst was studied. Production of monolaurin was optimized based on two selected types of CALB, varying enzyme concentrations and reaction time. and Aspergillus oryzae were used in this study. About 47.6% of monolaurin was synthesized using N-435 at a 5% enzyme concentration within 24 hours of reaction time. Characterizations were performed using different techniques namely gas chromatography-flame ionization detector (GC-FID), thin layer chromatography (TLC) and Fourier transform infrared spectroscopy (FTIR) analysis.Keywords: methyl laurate, enzymatic glycerolysis, monolaurin, Novozyme-435, Aspergillus oryzae Abstrak Tindak balas gliserolisis berenzim menggunakan lipase b Candida antarctica (CALB) telah dikaji. Penghasilan monolaurin dioptimumkan berasaskan dua jenis CALB, kepekatan enzim dan masa tindak balas. Novozym-435 (N-435) dan Aspergillus oryzae digunakan dalam kajian ini. Sebanyak 47.6% monolaurin telah berjaya disintesis menggunakan N-435 pada kepekatan enzim 5% dalam tempoh 24 jam masa tindak balas. Pencirian dijalankan menggunakan teknik analisis berbeza seperti kromatografi gas (GC), kromatografi lapisan nipis (TLC) dan spektroskopi inframerah transfomasi Fourier (FTIR).Kata kunci: metil laurat, gliserolisis berenzim, monolaurin, Novozym-435, Aspergillus oryzae Introduction Enzymatic glycerolysis reactions were reported to be carried out in a mild reaction condition, low temperature with solvents that are not harmful towards the environment. With the proliferated interest in green and moderate chemical reaction, enzymatic reaction is attractively favored in researches that produce edible products such as monoacylglycerol (MAG) and diacylglycerol (DAG) [1].
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