Biodiesel production from Nannochloropsis gaditana lipids through transesterification catalyzed by Rhizopus oryzae lipase

López, Elvira Navarro; Medina, Alfonso Robles; Moreno, Pedro A. González; Cerdán, Luis; Valverde, Lorena Martín; Grima, E. Molina

doi:10.1016/j.biortech.2015.12.036

Cited by 58 publications

(19 citation statements)

References 29 publications

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“…Subsequently, the conversion gradually decreased, reaching a conversion of around 70 mol% after the tenth reuse These results are comparable although better than others reported in the literature for the lipase from P. cepacia where after the first use, 10% of the activity was lost and in the fourth cycle the activity was reduced by 53% [41]. This loss of activity can be caused by a possible deterioration of the biocatalyst by enzyme poisoning [42].…”

Section: Study Of the Reuse Of The Heterogeneous Enzymatic Catalystsupporting

confidence: 80%

Biodiesel Production (FAEEs) by Heterogeneous Combi-Lipase Biocatalysts Using Wet Extracted Lipids from Microalgae

et al. 2019

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The production of fatty acids ethyl esters (FAEEs) to be used as biodiesel from oleaginous microalgae shows great opportunities as an attractive source for the production of renewable fuels without competing with human food. To ensure the economic viability and environmental sustainability of the microbial biomass as a raw material, the integration of its production and transformation into the biorefinery concept is required. In the present work, lipids from wet Isochrysis galbana microalga were extracted with ethyl acetate with and without drying the microalgal biomass (dry and wet extraction method, respectively). Then, FAEEs were produced by lipase-catalyzed transesterification and esterification of the extracted lipids with ethanol using lipase B from Candida antarctica (CALB) and Pseudomonas cepacia (PC) lipase supported on SBA-15 mesoporous silica functionalized with amino groups. The conversion to FAEEs with CALB (97 and 85.5 mol% for dry and wet extraction, respectively) and PC (91 and 87 mol%) biocatalysts reached higher values than those obtained with commercial Novozym 435 (75 and 69.5 mol%). Due to the heterogeneous nature of the composition of microalgae lipids, mixtures with different CALB:PC biocatalyst ratio were used to improve conversion of wet-extracted lipids. The results showed that a 25:75 combi-lipase produced a significantly higher conversion to FAEEs (97.2 mol%) than those produced by each biocatalyst independently from wet-extracted lipids and similar ones than those obtained by each lipase from the dry extraction method. Therefore, that optimized combi-lipase biocatalyst, along with achieving the highest conversion to FAEEs, would allow improving viability of a biorefinery since biodiesel production could be performed without the energy-intensive step of biomass drying.

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Section: Study Of the Reuse Of The Heterogeneous Enzymatic Catalystsupporting

confidence: 80%

Biodiesel Production (FAEEs) by Heterogeneous Combi-Lipase Biocatalysts Using Wet Extracted Lipids from Microalgae

et al. 2019

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“…Researches have shown that freshwater green algae Botryococcus braunii , Tetraselmis sp. , seawater algae Nannochloropsis sp., green algae Chorella sp., have high lipid content and considered as promising strains for biodiesel production . A few microalgal species like Spirulina , Chlorella , Dunaliella and Scenedesmus are considered as high protein resource and can be used as food protein by incorporating their biomass in protein rich food .…”

Section: Introductionmentioning

confidence: 99%

Biochemical characterization of microalgae collected from north east region of India advancing towards the algae‐based commercial production

Ghosh

Khanra

Mondal

et al. 2017

Asia-Pacific J Chem Eng

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Selection of suitable strain of microalgae is the crucial factor for large-scale production of algae-based products. Efforts have been made here for isolation, identification and biochemical characterization of five microalgae strains collected from Tripura (a small state in north-eastern region of India). Two Chlorococcum sp. (NITAAP008 and NITAAP019) demonstrate their high lipid (15-24%), equal amounts of carbohydrate and protein (35-40%), with specific growth rate of 0.13 day À1 . These strains are potential resource for biofuel production. After lipid extraction, remaining biomass can be used as source of carbohydrate for the production of other biofuels. One isolated strain is identified as Chlorella sp. (NITAAP009) and shows 22-33% carbohydrate, 41-50% protein and 5% chlorophyll with specific growth rate of 0.125 day À1 . Another Chlorella sp. (NITAAP011) isolated from lake area exhibits significant chlorophyll (5-6.4%), 30-50% carbohydrate, 48-60% protein and low lipid (1-10%) with lower specific growth rate (0.10 day À1 ). Both strains are having industrial competence for chlorophyll production due to their synthesizing ability of significant amount of chlorophyll (5-6.4%). The last one, Korshikoviella sp. (NITAAP017), has 15-18% lipid, 22-34% carbohydrate, 30-43% protein and 3-4% chlorophyll with specific growth rate of 0.12 day À1 and can be used for food supplement production or lipid synthesis.

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“…shows the variation in the FAME conversion with reaction time achieved in three consecutive transesterification reactions catalyzed by the same R. oryzae batch.These reactions were carried out under the conditions established as optimal for this lipase in a previous work (NavarroLópez et al, 2016), and after each reaction cycle the lipase was filtrated and washed with t-butanol before use, using 50 mL of t-butanol/g lipase. Figures 6a-d show that both reaction velocity (lipase activity) and final conversion attained at 72 h decrease with the number of lipase uses.…”

mentioning

confidence: 99%

Extraction of microalgal lipids and the influence of polar lipids on biodiesel production by lipase-catalyzed transesterification

López

Medina

Moreno

et al. 2016

Bioresource Technology

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Biodiesel production from Nannochloropsis gaditana lipids through transesterification catalyzed by Rhizopus oryzae lipase

Cited by 58 publications

References 29 publications

Biodiesel Production (FAEEs) by Heterogeneous Combi-Lipase Biocatalysts Using Wet Extracted Lipids from Microalgae

Biodiesel Production (FAEEs) by Heterogeneous Combi-Lipase Biocatalysts Using Wet Extracted Lipids from Microalgae

Biochemical characterization of microalgae collected from north east region of India advancing towards the algae‐based commercial production

Extraction of microalgal lipids and the influence of polar lipids on biodiesel production by lipase-catalyzed transesterification

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