Biodiesel production from canola oil by using lipase immobilized onto hydrophobic microporous styrene–divinylbenzene copolymer

Dizge, Nadir; Keskinler, Buelent; Tanrıseven, Aziz

doi:10.1016/j.bej.2008.12.008

Cited by 108 publications

(57 citation statements)

References 25 publications

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“…C-4 lipase was immobilized by cross-linking of the enzyme molecules with gluteraldehyde and adsorption onto Diaion HP-20 beads, comprising of a hydrophobic Sty-Dvb resin (6). The hydrophobicity of the resin can impede penetration of water into its pores.…”

Section: Lipase Immobilizationmentioning

confidence: 99%

Bacillus sp. PS35 Lipase-Immobilization on Styrene-Divinyl Benzene Resin and Application in Fatty Acid Methyl Ester Synthesis

Palanisamy¹,

Kumaresan²,

Jeyaseelan³

2015

Iran J Biotech

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Background:Lipase is an enzyme with immense application potential. Ester synthesis by lipase catalysis in organic media is an area of key industrial relevance. Enzymatic preparations with traits that cater to the needs of this function are hence being intensely researched. Objective: The objectives of the study were to immobilize the lipase from Bacillus sp. PS35 by cross-linking and adsorption onto styrene-divinyl benzene (Sty-Dvb) hydrophobic resin and to comparatively characterize the free and immobilized lipase preparations. The work also aimed to apply the immobilized lipase for catalysing the fatty acid methyl ester (FAME) synthesis from palm oil and optimize the process parameters for maximizing the yield. Materials and Methods: In this study, the purified lipase from Bacillus sp. PS35 was immobilized by adsorption onto styrene-divinyl benzene hydrophobic resin with gluteraldehyde cross-linking. Results: The immobilized enzyme showed better pH and temperature stabilities than the free lipase. Organic solvent stability was also enhanced, with the relative activity in the presence of methanol being shifted from 53% to 81%, thereby facilitating the enzyme's application in fatty acid methyl ester synthesis. It exhibited remarkable storage stability over a 30-day period and after 20 repetitive uses. Cross-linking also reduced enzyme leakage by 49%. The immobilized lipase was then applied for biodiesel production from palm oil. Methanol and oil molar ratio of 5:1, three step methanol additions, and an incubation temperature of 50°C were established to be the ideal conditions favoring the transesterification reaction, resulting in 97% methyl ester yield. Conclusions: These promising results offer scope for further investigation and process scale up, permitting the enzyme's commercial application in a practically feasible and economically agreeable manner.

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Section: Lipase Immobilizationmentioning

confidence: 99%

Bacillus sp. PS35 Lipase-Immobilization on Styrene-Divinyl Benzene Resin and Application in Fatty Acid Methyl Ester Synthesis

Palanisamy¹,

Kumaresan²,

Jeyaseelan³

2015

Iran J Biotech

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“…Biodiesel is characterised in generations, namely, first, second and third generation [26]. First generation biodiesel is produced from edible food crops such as mustard oil [27,28], canola oil [29], sunflower oil etc. [30,31].…”

Section: Introductionmentioning

confidence: 99%

Comparative study of diesel engine performance and emission with soybean and waste oil biodiesel fuels

Azad¹,

Rasul²,

Giannangelo³

et al. 2015

Int. J. Automot. Mech. Eng.

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The study investigated the engine performance and emission using biodiesel from soybean oil and waste cooking oil. The fuel properties of these biodiesels were determined using ASTM D6751and EN 14214 standards. The fuels were tested in a multi-cylinder diesel engine with an electromagnetic dynamometer and a 5-gas analyser was used for emission analysis. The result shows that the thermal performance of the engine slightly decreases with increase of the biodiesel blends ratio (i.e. B5, B10, B15, B20 and B50). On the other hand, emission decreases with the increase of biodiesel blends. The reduction in emissions was different for both biodiesels. Waste oil biodiesel showed better trends of emissions reduction with increase of the biodiesel percentage compared with soybean biodiesel. The brake specific fuel consumption (BSFC) of both biodiesels is (6.05%, 9.13%, 12.36% and 18.57% for soybean) and (8.17%, 11.40%, 17.71% and 14.96% for waste cooking oil) higher than diesel, respectively. However, soybean biodiesel consumed less fuel and produced more power and torque compared to waste oil biodiesel. The study concluded that B10 soybean biodiesel blend produced more consistent and expected results than waste oil biodiesel from the performance and emission point of view.

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“…The immobilized enzyme retained full activity after 30 days storage under open bench conditions and retained its full activity during the 15 batches of replicated biodiesel syntheses. Later the same groups used this biocatalyst to produce biodiesel using methanol and canola oil (97% yield in 24 h of reaction) [28]. Comparing the use of PSD and PSD-activated with polyglutaraldehyde, immobilization yield improved from 60% to 85%…”

Section: Immobilization Of Enzymes Via Covalent Attachmentmentioning

confidence: 99%

Immobilization of Proteins in Poly-Styrene-Divinylbenzene Matrices: Functional Properties and Applications

Rafael¹,

Hernández²,

Barbosa³

et al. 2015

COC

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Supports based on poly-styrene-divinylbenzene (PSD) are commercially available since a long time ago. However, they are not commonly used as enzyme immobilization matrices. The main reason for this lies in the negative effect of the very hydrophobic surface on enzyme stability that produces the instantaneous enzyme inactivation in many instances. However, they have recently regained some impact on enzyme immobilization. They are easy to modify, and have been prepared with different modifiers. We will pay special attention to the coating of these supports with ionic liquids, which permits to have the ionic liquid phase anchored to the solid and modulate the enzyme properties without risk of losing these expensive and potentially toxic compounds. Thus, this review will present the covalent or physical immobilization of enzymes on PSD supports, submitted to different modifications. Moreover, lipases immobilized via interfacial activation on some naked PSD supports have shown some unexpected improvement in their catalytic properties, with uses in reactions like hydrolysis, esterification or transesterification.

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Biodiesel production from canola oil by using lipase immobilized onto hydrophobic microporous styrene–divinylbenzene copolymer

Cited by 108 publications

References 25 publications

Bacillus sp. PS35 Lipase-Immobilization on Styrene-Divinyl Benzene Resin and Application in Fatty Acid Methyl Ester Synthesis

Bacillus sp. PS35 Lipase-Immobilization on Styrene-Divinyl Benzene Resin and Application in Fatty Acid Methyl Ester Synthesis

Comparative study of diesel engine performance and emission with soybean and waste oil biodiesel fuels

Immobilization of Proteins in Poly-Styrene-Divinylbenzene Matrices: Functional Properties and Applications

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