“…The biodesulfurization processes were carried out in 250 mL flasks with 100 mL basic salt medium, 10% W/V pulp density, particle size of −65 Tyler mesh, and the initial cell concentration was 1.0 × 10 6 cells·mL −1 , and processing time of 24 d. Every four days, the pH values, Eh (oxidation potential), and total iron concentration of biodesulfurization system were determined. At the end of the experiments, the residual coal samples were prepared with the method mentioned in the literature [ 23 ], and the proximate analysis, total sulfur, and the relative sulfur contents in the coal samples were then measured as the method mentioned in Section 2.1 . For pyrite bioleaching experiments, 250 mL flasks containing 100 mL sterilized culture medium and 1.0 g pyrite (particle size of −65 Tyler mesh) were incubated with cells (the initial cells concentration 1.0 × 10 6 cells/mL) on a rotary shaker at 170 rpm, 30°C.…”