Biodeinking of mixed office waste paper by alkaline active cellulases from alkalotolerant Fusarium sp.

Vyas, S. P.; Lachke, Anil

doi:10.1016/s0141-0229(02)00273-9

Cited by 73 publications

(32 citation statements)

References 22 publications

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“…This slightly difference in the brightness increment probably due to the 10 min interval time before each of the enzymes added was too short to show the differences. The increased in brightness or toner removal was due to action of cellulase (endoglucanase), which released fibers from the surface of hairy toners, which in turn enhanced the flotation efficiencies by increasing hydrophobicity of ink particles (Vyas & Lachke, 2003). Similar increased in brightness was observed for the enzyme sequence of HLC and LCH (1.40%).…”

Section: Effect Of Different Enzymes Sequences On Ink Removalmentioning

confidence: 67%

Characterization of cellulase, hemicellulase and lipase and its use in deinking of laser printed paper

Lee¹,

Demirtaş²,

Omar³

2013

MJM

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Aims: It is recognized that laser printed paper are difficult to deink using conventional method. This had lead to the suggestion of enzymatic approach to overcome the problem encountered by commonly employed deinking techniques. The present study aimed to investigate 7 commercially available enzymes for their suitability use in deinking of laser printed paper. Methodology and results: 3 cellulases, hemicellulases, xylanase and 2 lipases were used in enzymatic deinking of laser-printed wastepaper. Cellulase A "Amano"3 (C), Hemicellulase (H) and lipase (L) were selected for used in deinking because they possess either highest activity or broad pH stability compared to others enzymes. Different combination of enzymes was carried out to evaluate their effectiveness in deinking process. CH enzymes sequence was determined to be the most effective sequence in toner removal with 1.90% of brightness increment. However, only 0.95% of brightness increment was gained by enzyme sequence L. Highest deinking efficiency obtained was not proportional to the highest total reducing sugar produced. Conclusion, significance and impact of study: Enzyme (cellulase and hemicellulase) can be used to de-ink laserprinted wastepaper, which are difficult to be deinked by conventional chemical deinking process. Thus, enzyme deinking has high possibility as alternative method to current chemical deinking process which is not environmental friendly.

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Section: Effect Of Different Enzymes Sequences On Ink Removalmentioning

confidence: 67%

Characterization of cellulase, hemicellulase and lipase and its use in deinking of laser printed paper

Lee¹,

Demirtaş²,

Omar³

2013

MJM

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“…Because endoglucanase and lipase have shown high deinking efficiency (Gübitz et al 1998;Morkbak et al 1999;Marques et al 2003;Pelach et al 2003;Vyas and Lachke 2003), the authors assumed that the combined use of two enzymes could promote better deinking efficiency. With such assumption, a chimeric enzyme with endoglucanase and lipase activities was constructed in this study by an end-to-end fusion technique, in which the Lip catalytic domain at N-terminus was fused with the EG1 catalytic domain at C-terminus (Khandeparker and Numan 2008).…”

Section: Design Of the Chimera Lip-eg1cdmentioning

confidence: 99%

“…Waste papers are now the major fiber resources for paper manufacturers in China and other countries (Vyas and Lachke 2003). In the waste paper recycling process, the printed ink in the paper needs to be removed by a deinking process.…”

Section: Introductionmentioning

confidence: 99%

The Enzymatic Deinking of Waste Papers by Engineered Bifunctional Chimeric Neutral Lipase – Endoglucanase

Liu¹,

Long²,

Wu³

et al. 2017

BioResources

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Endoglucanase and lipase showed good deinking efficiency for waste papers. The performances could be greatly improved further by the combined use of the two enzymes. To reduce the enzyme production cost and enhance synergistic action of endoglucanase and lipase on laser-printed paper and newspaper, a chimeric enzyme with endoglucanase and lipase activity was constructed and expressed in Pichia pastoris. The data indicated that the chimera Lip-EG1CD improved the ink removal efficiencies and sheet brightness better than a single enzyme or a mixture of two enzymes. The chimera Lip-EG1CD demonstrated an 89% removal of toner on both papers and 91% ISO and 60% ISO sheet brightness for laser-printed paper and newspaper, respectively. Handsheet strength was also clearly improved. It revealed that the combined deinking of endoglucanase and lipase on waste papers could be strengthened by constructing proper chimera due to intramolecular synergistic action. This would be useful for developing an economical process for waste paper recycling.

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“…As celulases apresentam aplicação nas indústrias de papel e celulose, têxtil, sucos de frutas, álcool e detergentes, dentre outras. [15][16][17][18] A Figura 1 apresenta a reação geral de hidrólise da celulose, onde a unidade em destaque na fibra (entre colchetes) representa a unidade de repetição, celobiose.…”

Section: Celulasesunclassified

Application of Enzymes: Proposals for an Experimental Class

Ribeiro¹,

Castro²,

Salgado³

et al. 2013

Revista Virtual De Química

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Rev. Virtual Quim. |Vol 5| |No. 5| |787-805| Application of Enzymes: Proposals for an Experimental ClassAbstract: Currently, several groups of enzymes belonging to the classes of oxidoreductases and hydrolases have been applied as clean alternatives for many processes in food, detergents, textiles and pharmaceutical industries. The objective of this article is to present new approaches for undergraduate experimental classes based on simple and rapid methodologies, concerning the application of enzymes (cellulases, hemicellulases, pectinases, proteases, ureases and tyrosinases). The practices are adequated to be used in disciplines in the areas of enzyme technology and food technology, among others. Keywords:Enzymes; hydrolysis; biocatalysis. ResumoDiversos grupos de enzimas das classes das oxidorredutases e hidrolases são aplicados como alternativas limpas em muitos processos nas indústrias de alimentos, detergentes, têxteis e fármacos. O objetivo desse artigo é apresentar novas abordagens para disciplinas experimentais do ensino superior, baseadas em metodologias simples e rápidas, para a aplicação de alguns grupos de enzimas (celulases, hemicelulases, pectinases, proteases, ureases e tirosinases). As práticas são adequadas para serem oferecidas em disciplinas nas áreas de tecnologia enzimática e tecnologia de alimentos, dentre outras. Palavras-chave:Enzimas; hidrólise; biocatálise.

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Biodeinking of mixed office waste paper by alkaline active cellulases from alkalotolerant Fusarium sp.

Cited by 73 publications

References 22 publications

Characterization of cellulase, hemicellulase and lipase and its use in deinking of laser printed paper

Characterization of cellulase, hemicellulase and lipase and its use in deinking of laser printed paper

The Enzymatic Deinking of Waste Papers by Engineered Bifunctional Chimeric Neutral Lipase – Endoglucanase

Application of Enzymes: Proposals for an Experimental Class

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