Biodegradation of α-endosulfan via hydrolysis pathway by Stenotrophomonas maltophilia OG2

Abstract

Stenotrophomonas maltophilia OG2 was isolated from the intestine of cockroaches that was collected from a cow barn contaminated some pesticides belong to pyrethroid and organochlorine groups. OG2 was able to degrade a-endosulfan in non sulfur medium (NSM) as a sole sulfur source for growth within 10 days of incubation. The effects of some growth parameters on endosulfan biodegradation by OG2 was studied and found that the biodegradation was significantly affected by the endosulfan concentrations, pH and temperature. Experimental results obtained in different conditions show that the optimum concentration of a-endosulfan, pH and temperature were 100 mg/L, 8.0 and 30°C, respectively. Under these conditions, the bacterium degraded 81.53% of the a-endosulfan after 10 days. The concentration of a-endosulfan and its metabolites was determined by HPLC. Endosulfan ether, endosulfan lactone and endosulfan diol were the main metabolites in culture, but did not produce toxic metabolite, endosulfan sulfate. These results suggested that S. maltophilia OG2 degrades a-endosulfan via a hydrolysis pathway. The present study indicates that strain OG2 may have potential use in the biodegradation of pesticides contaminated environments.

Isolation and identification of marine strains ofStenotrophomona maltophiliawith high chitinolytic activity

“…Contamination from the extensive release of organic residues by the industry, the accumulation of decomposing organisms, the presence of hydrocarbons from the use of fuels, and the presence of agrochemicals create favorable conditions for the growth of S. maltophilia. According to some reports, S. maltophilia is frequently present in environments polluted with hydrocarbons, pesticides and heavy metals, and its isolation is possible from these locations (Alfonso et al, 2016; Mukherjee & Roy, 2016; Ozdal et al, 2017). The identification, sequencing or use of the genetic material of these microorganisms could represent a biotechnological alternative to develop nonpathogenic microorganisms capable of degrading chitin.…”

Isolation and identification of the pyrethroid insecticide deltamethrin degrading bacteria from insects

“…Chlorpyrifos and fipronil resistant strains of diamond back moth (Plutella xylostella) have higher levels of Lactobacillales, Pseudomonadales and Xanthomonadales compared to susceptible insects (Xia et al, 2013). Stenotrophomonas maltophilia OG-2, isolated from the intestine of the cockroach (Blatta orientalis), can degrade both α-endosulfan (Ozdal et al, 2017) and synthetic pyrethroid α-cypermethrin (Gur et al, 2014).…”

“…Pesticide References Gaonkar et al, 2019 Stenotrophomonas maltophilia α-endosulfan, α-cypermethrin Gur et al, 2014;Ozdal et al, 2017 DDT Neerja, Grewal et al, 2016Flavimonas oryzihabitans DDT Barragan-Huerta et al, 2007…”

“…Biodegradation is an environment friendly, cheap and high efficiency approach compared to other methods. Bacteria and fungi with high enzyme (transferases, isomerases, ligases and hydrolases especially esterases, peroxidases and oxygenases) activity are used in biodegradation studies (Ortiz-Hernández et al, 2013;Ozdal et al, 2017).…”

Endosulfan removal through bioremediation, photocatalytic degradation, adsorption and membrane separation processes: A review

“…Technically, endosulfan contains two isomers, αendosulfan and β-endosulfan. In the environment, soil or water, both endosulfan isomers are slowly degraded to endosulfan sulfate and then to other products such as chloride ions [19], endosulfan diol [20,21], endosulfan ether and endosulfan lactone [21][22][23], endosulfan diol monosulfate [24] and 2 heptanone, dieldrin and methyl propionate [25].…”