Biodegradation of Triton X-100 and its primary metabolites by a bacterial community isolated from activated sludge

Wyrwas, Bogdan; Dymaczewski, Zbysław; Zgoła‐Grześkowiak, Agnieszka; Szymański, Andrzej; Frańska, Magdalena; Kruszelnicka, Izabela; Ginter-Kramarczyk, Dobrochna; Cyplik, Paweł; Ławniczak, Łukasz; Chrzanowski, Łukasz

doi:10.1016/j.jenvman.2013.05.028

Cited by 26 publications

(11 citation statements)

References 32 publications

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“…These interactions function as barriers that prevent A. niger from accessing the substrate, and thus stop, delay, or slow down its growth because of food deficit. Furthermore, the interactions between Triton X-100 and A. niger can cause the erosion of the cell membrane of A. niger Another possible mechanism employed for growth suppression is the toxicity of the intermediate products produced during the primary biodegradation of Triton [31]. Koley and Bard [32] reported irreversible damage to HeLa cells, in less than 1 min, in the presence of 130 mg/L Triton X-100; this damage can be attributed to the solubilization of the cellular lipid bilayers because of their interactions with Triton.…”

Section: Discussionmentioning

confidence: 99%

Aspergillus niger-mediated bioremediation of Triton X-100-contaminated resources

Abu-Ghunmi

Al-Khatib

Alkhawaldeh

2015

Desalination and Water Treatment

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A B S T R A C TWater shortage, water pollution and climate changes are highly interrelated global issues. These have raised immense concerns about possible serious adverse effects on the quality, treatment and reuse of wastewater. This research aims to investigate the potential impact of Triton X-100 on the soilborne fungus Aspergillus niger (A. niger) as well as the ability of this organism to remove Triton X-100 from contaminated wastewater. We found that 2,250-6,000 mg/L Triton is toxic toward A. niger with irreversible effects, whereas 23-2,000 mg/L is inhibitory toward A. niger with reversible effects. These concentration ranges vary with initial concentrations of Triton and A. niger as well as with the contact time. At an initial concentration of 228 and 2,152 mg/L, A. niger can remove 20 and 28%, respectively, of Triton by day 1, which increase to 28 and 38%, respectively, by day 3. Another experiment showed that at an initial concentration of 50 and 100 mg/L, A. niger can biodegrade 29 and 15%, respectively, of Triton by day 1, which further increases to 87 and 34% by day 15. Adsorption and biodegradation are the mechanisms employed by the organism for Triton removal. Thus, A. niger can enhance biological treatment of wastewater.

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Section: Discussionmentioning

confidence: 99%

Aspergillus niger-mediated bioremediation of Triton X-100-contaminated resources

Abu-Ghunmi

Al-Khatib

Alkhawaldeh

2015

Desalination and Water Treatment

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“…This can be attributed in part to the fact that these environments typically contain a variety of different PAH degrading microorganisms with different environmental niches and metabolic strategies, metabolic pathways and substrate ranges (Janssen 2006). In addition, there are other external factors as the presence of the fungi and surfactants, which can affect the microbial community (Wyrwas et al 2013;Szczepaniak et al 2016). …”

Section: The Emergence Of Different Functionally Equivalent Pah Degramentioning

confidence: 99%

The Emergence of Different Functionally Equivalent PAH Degrading Microbial Communities from a Single Soil in Liquid PAH Enrichment Cultures and Soil Microcosms Receiving PAHs with and without Bioaugmentation

Piubeli

Santos

Fernández

et al. 2018

Polish Journal of Microbiology

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A b s t r a c tPolycyclic aromatic hydrocarbon (PAHs) are common soil contaminants of concern due to their toxicity toward plants, animals and microorganisms. The use of indigenous or added microbes (bioaugmentation) is commonly used for bioremediation of PAHs. In this work, the biodegradation rates and changes in the bacterial community structure were evaluated. The enrichment culture was useful for unambiguously identifying members of the soil bacterial community associated with PAH degradation and yielded a low diversity community. No significant difference in the rate of PAH degradation was observed between the microcosm receiving only PAHs or PAHs and bioaugmentation. Moreover, identical matches to the bioaugmentation inoculum were only observed at the initial stages of PAH degradation on day 8. After 22 days of incubation, the substantial degradation of all PAHs had occurred in both microcosms and the PAH contaminated soil had statistically significant increases in Alphaproteobacteria. There were also increases in Betaproteobacteria. In contrast, the PAH contaminated and bioaugmented soil was not enriched in PAH degrading Proteobacteria genera and, instead, an increase from 1.6% to 8% of the population occurred in the phylum Bacteroidetes class Flavobacteria, with Flavobacterium being the only identified genus. In addition, the newly discovered genus Ohtaekwangia increased from 0% to 3.2% of the total clones. These results indicate that the same soil microbial community can give rise to different PAH degrading consortia that are equally effective in PAH degradation efficiency. Moreover, these results suggest that the lack of efficacy of bioaugmentation in soils can be attributed to a lack of persistence of the introduced microbes, yet nonetheless may alter the microbial community that arises in response to PAH contamination in unexpected ways.

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“…Due to their common discharge into the environment, surfactants are among the most important environmental pollutants (Wyrwas et al 2013;Menzies et al 2017). They often exhibit potent biological activity, i.e., interaction with cellular membranes and disruption of important biochemical processes, and may therefore negatively affect living organisms (especially in case of marine environments) (Wyrwas et al 2011;Rebello et al 2014;Pereira et al 2015).…”

Section: Introductionmentioning

confidence: 99%

How to accurately assess surfactant biodegradation-impact of sorption on the validity of results

Cierniak

Woźniak-Karczewska

Parus

et al. 2019

Appl Microbiol Biotechnol

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Surfactants not only are widely used in biotechnological processes but also constitute significant contaminants of the modern world. Among many reports, there is a shortage of works which summarize the issue of surfactant sorption to biomass in a way that would elucidate the biological factors for analysts and analytical factors for microbiologists. The main factor, which is not as obvious as one would expect, is associated with the susceptibility of analytical approaches to errors resulting from incorrect handling of biomass. In case of several publications reviewed in the framework of this study, it was not possible to establish whether the decrease of the analytical signal observed by the authors actually resulted from biodegradation of the surfactant. This review emphasizes the necessity to consider the possibility of surfactant sorption to microbial cells, which may result in significant detection errors as well as conceptual inconsistency. In addition, a reference study regarding representative surfactants (cationic, anionic and non-ionic) as well as yeast, Gram-negative, Gram-positive bacteria, and activated sludge was provided to highlight the possible errors which may arise from disregarding sorption processes when determining degradation of surfactants. This particularly applies to systems which include ionic surfactants and activated sludge as sorption may account for 90% of the observed depletion of the surfactant. Therefore, a systematic approach was proposed in order to improve the credibility of the obtained results. Finally, the need to employ additional procedures was highlighted which may be required in order to verify that the decrease of surfactant concentration results from biodegradation processes.

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Biodegradation of Triton X-100 and its primary metabolites by a bacterial community isolated from activated sludge

Cited by 26 publications

References 32 publications

Aspergillus niger-mediated bioremediation of Triton X-100-contaminated resources

Aspergillus niger-mediated bioremediation of Triton X-100-contaminated resources

The Emergence of Different Functionally Equivalent PAH Degrading Microbial Communities from a Single Soil in Liquid PAH Enrichment Cultures and Soil Microcosms Receiving PAHs with and without Bioaugmentation

How to accurately assess surfactant biodegradation-impact of sorption on the validity of results

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