Biodegradation of the pyridinecarboxamide insecticide flonicamid by Microvirga flocculans and characterization of two novel amidases involved

Zhao, Yun-Xiu; Guo, Ling; Wang, Li; Jiang, Nengdang; Chen, Kexin; Dai, Yinming

doi:10.1016/j.ecoenv.2021.112384

Cited by 22 publications

(15 citation statements)

References 48 publications

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“…A characteristic fragment (m/z = 146) of TFNA, C 6 H 5 NF 3 , was also found. 42 Both the retention time of A2 in HPLC and its relative molecular mass were consistent with those of TFNA. These findings demonstrated that TFNA was the metabolite of TFNA-AM degradation by immobilized P. putida KT2440 pLSJ15-amiA.…”

Section: Metabolite Identificationsupporting

confidence: 54%

Rapid Degradation of Hazardous Amides by Immobilized Engineered Pseudomonas putida KT2440 Based on a Novel Gene Expression Vector

Jiang,

Yu,

Guo

et al. 2024

J. Agric. Food Chem.

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The amides 4-trifluoromethylnicotinamide, acrylamide, and benzamide are widely used in agriculture and industry, posing hazards to the environment and animals. Immobilized bacteria are preferred in wastewater treatment, but degradation of these amides by immobilized engineered bacteria has not been explored. Here, engineered Pseudomonas putida KT2440 pLSJ15-amiA was constructed by introducing a new amidase gene expression vector into environmentally safe P. putida KT2440. P. putida KT2440 pLSJ15-amiA had high amidase activity, even at 80 °C. P. putida KT2440 pLSJ15-amiA immobilized with calcium alginate exhibited a greater environmental tolerance than free cells. The amides were rapidly degraded by the immobilized cells, but the activity was inhibited by high concentrations of substrates. The substrate inhibition model revealed that the optimum initial concentrations of 4-trifluoromethylnicotinamide, acrylamide, and benzamide for degradation by immobilized cells were 197.65, 350.76, and 249.40 μmol/L, respectively. This study develops a novel and excellent immobilized biocatalyst for remediation of wastewater containing hazardous amides.

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Section: Metabolite Identificationsupporting

confidence: 54%

Rapid Degradation of Hazardous Amides by Immobilized Engineered Pseudomonas putida KT2440 Based on a Novel Gene Expression Vector

Jiang,

Yu,

Guo

et al. 2024

J. Agric. Food Chem.

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“…They are metabolically versatile and widely distributed in nature 51 . In addition, Microvirga is also reported to be an environmental remediator, as it can remove residual contaminants in soil and water 52 . Pedomicrobium is a ubiquitously occurring hyphal-budding organism that can adhere to surfaces and form biofilms in different habitats 53 .…”

Section: Discussionmentioning

confidence: 99%

Taxonomic response of bacterial and fungal populations to biofertilizers applied to soil or substrate in greenhouse-grown cucumber

Shi

Zhu

et al. 2022

Sci Rep

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Reductions in the quality and yield of crops continuously produced in the same location for many years due to annual increases in soil-borne pathogens. Environmentally-friendly methods are needed to produce vegetables sustainably and cost effectively under protective cover. We investigated the impact of biofertilizers on cucumber growth and yield, and changes to populations of soil microorganisms in response to biofertilizer treatments applied to substrate or soil. We observed that some biofertilizers significantly increased cucumber growth and decreased soil-borne pathogens in soil and substrate. Rhizosphere microbial communities in soil and substrate responded differently to different biofertilizers, which also led to significant differences in microbial diversity and taxonomic structure at different times in the growing season. Biofertilizers increase the prospects of re-using substrate for continuously producing high-quality crops cost-effectively from the same soil each year while at the same time controlling soil-borne disease.

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“…Incubation was performed at 30 °C and 220 rpm, followed by sampling at a defined time and analysis by HPLC. LOD (limit of detection) and LOQ (limit of quantification) are used as reliability validation methods for FLO residue detection by HPLC (Zhao et al, 2021a).…”

Section: Methodsmentioning

confidence: 99%

“…Previously, Aminobacter sp. CGMCC 1.17253 (Yang et al, 2021a), Ensifer meliloti CGMCC 7333 (Yang et al, 2021b), Ensifer adhaerens CGMCC 6315 (Zhao et al, 2021b), and Microvirga flocculans CGMCC 1.16731(Zhao et al, 2020; Zhao et al, 2021a) have been reported to degrade FLO via NHase/amidase pathway. Nevertheless, FLO degradation by nitrilase (EC 3.5.5.1) has been poorly understood.…”

Section: Introductionmentioning

confidence: 99%

Characterization of nitrilases from Variovorax boronicumulans that functions in insecticide flonicamid degradation and β-cyano-L-alanine detoxification

Jiang

Wang

et al. 2022

Journal of Applied Microbiology

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Aims: To characterize the functions of nitrilases of Variovorax boronicumulans CGMCC 4969 and evaluate flonicamid (FLO) degradation and β-cyano-L-alanine (Ala(CN)) detoxification by this bacterium. Methods and Results: Variovorax boronicumulans CGMCC 4969 nitrilases (NitA and NitB) were purified, and substrate specificity assay indicated that both of them degraded insecticide FLO to N-(4-trifluoromethylnicotinoyl)glycinamide (TFNG-AM) and 4-(trifluoromethyl)nicotinol glycine (TFNG). Ala(CN), a plant detoxification intermediate, was hydrolysed by NitB. Escherichia coli overexpressing NitA and NitB degraded 41.2 and 93.8% of FLO (0.87 mmol•L −1 ) within 1 h, with half-lives of 1.30 and 0.25 h, respectively. NitB exhibited the highest nitrilase activity towards FLO. FLO was used as a substrate to compare their enzymatic properties. NitB was more tolerant to acidic conditions and organic solvents than NitA. Conversely, NitA was more tolerant to metal ions than NitB. CGMCC 4969 facilitated FLO degradation in soil and surface water and utilized Ala(CN) as a sole nitrogen source for growth. Conclusions: CGMCC 4969 efficiently degraded FLO mediated by NitA and NitB; NitB was involved in Ala(CN) detoxification. Significance and Impact of the Study: This study promotes our understanding of versatile functions of nitrilases from CGMCC 4969 that is promising for environmental remediation.

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Biodegradation of the pyridinecarboxamide insecticide flonicamid by Microvirga flocculans and characterization of two novel amidases involved

Cited by 22 publications

References 48 publications

Rapid Degradation of Hazardous Amides by Immobilized Engineered Pseudomonas putida KT2440 Based on a Novel Gene Expression Vector

Rapid Degradation of Hazardous Amides by Immobilized Engineered Pseudomonas putida KT2440 Based on a Novel Gene Expression Vector

Taxonomic response of bacterial and fungal populations to biofertilizers applied to soil or substrate in greenhouse-grown cucumber

Characterization of nitrilases from Variovorax boronicumulans that functions in insecticide flonicamid degradation and β-cyano-L-alanine detoxification

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