Rapid Degradation of Hazardous Amides by Immobilized Engineered Pseudomonas putida KT2440 Based on a Novel Gene Expression Vector
Huoyong Jiang,
Xuexiu Yu,
Jingjing Guo
et al.
Abstract:The amides 4-trifluoromethylnicotinamide, acrylamide,
and benzamide
are widely used in agriculture and industry, posing hazards to the
environment and animals. Immobilized bacteria are preferred in wastewater
treatment, but degradation of these amides by immobilized engineered
bacteria has not been explored. Here, engineered Pseudomonas
putida KT2440 pLSJ15-amiA was constructed by introducing
a new amidase gene expression vector into environmentally safe P. putida KT2440. P. putida KT2440 pLSJ15-amiA had high … Show more
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