Biodegradation of the plastics PLA and PET in cultivated soil with the participation of microorganisms and plants

Janczak, Katarzyna; Dąbrowska, Grażyna B.; Raszkowska-Kaczor, Aneta; Kaczor, Daniel; Hrynkiewicz, Katarzyna; Richert, Agnieszka

doi:10.1016/j.ibiod.2020.105087

Cited by 78 publications

(34 citation statements)

References 38 publications

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“…The aliphatic, aromatics, polyaromatic hydrocarbon, phthalate, polyethylene, and polyisoprene degradation genes were reported in the Rhodococcus ( Zampolli et al, 2019 ). Janczak et al (2020) demonstrated the ability of rhizospheric bacterial strain ( Serratia plymuthica ) for degradation of PET in cultivated and compost soil, as well as the presence of 155 genes for xenobiotics biodegradation in the genome.…”

Section: Introductionmentioning

confidence: 99%

Transcriptome-Guided Insights Into Plastic Degradation by the Marine Bacterium

et al. 2021

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Polyethylene terephthalate (PET) is a common single-use plastic that accumulated in the environment because of its non-degradable characteristics. In recent years, microbes from different environments were found to degrade plastics and suggested their capability to degrade plastics under varying environmental conditions. However, complete degradation of plastics is still a void for large-scale implications using microbes because of the lack of knowledge about genes and pathways intricate in the biodegradation process. In the present study, the growth and adherence of marine Bacillus species AIIW2 on PET surface instigating structural deterioration were confirmed through weight loss and hydrophobicity reduction, as well as analyzing the change in bond indexes. The genome-wide comparative transcriptomic analysis of strain AIIW2 was completed to reveal the genes during PET utilization. The expression level of mRNA in the strain AIIW2 was indexed based on the log-fold change between the presence and absence of PET in the culture medium. The genes represent carbon metabolism, and the cell transport system was up-regulated in cells growing with PET, whereas sporulation genes expressed highly in the absence of PET. This indicates that the strain AIIW2 hydrolyzes PET and assimilated via cellular carbon metabolism. A protein–protein interaction network was built to obtain the interaction between genes during PET utilization. The genes traced to degrade PET were confirmed by detecting the hydrolytic product of PET, and genes were cloned to improve PET utilization by microbial system as an eco-friendly solution.

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Section: Introductionmentioning

confidence: 99%

Transcriptome-Guided Insights Into Plastic Degradation by the Marine Bacterium

et al. 2021

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“… Janczak et al (2020) have conducted similar research work using other bacterial strains ( Janczak et al, 2020 ). B. cereus has been shown increase biomass production of Trifolium repens ( Azcon et al, 2010 ) and Salix viminalis ( Hrynkiewicz et al, 2012 ).…”

Section: Resultsmentioning

confidence: 98%

“…The formation of a biofilm by bacteria is the first step in the biodegradation of polymers ( Richert & Da̧browska, 2021 ; Janczak et al, 2020 ). Figure 1 shows the results of biofilm formation by Bacillus strains.…”

Section: Resultsmentioning

confidence: 99%

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Combined use of Bacillus strains and Miscanthus for accelerating biodegradation of poly(lactic acid) and poly(ethylene terephthalate)

Dąbrowska

Janczak²,

Richert

2021

PeerJ

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Background The aim of this study was to verify whether the presence of Bacillus strains and of miscanthus influence biodegradation and formed of biofilm of poly(lactic acid) (PLA) and poly(ethylene terephthalate) (PET). Methods The experiment conducted in compost soil showed that strains Bacillus subtilis and Bacillus cereus isolated from heavy metal contaminated environment have biochemical activity and accelerate biodegradation of both plastic materials. Results For PLA film it was found that the carbonyl index dropped by over 15% in the presence of B. subtilis, while the film tensile strength decreased by 35% and the oxygen to carbon O/C ratio was higher by 3% in the presence of B. cereus, and the presence of miscanthus resulted in a loss of weight. For PET film, a decrease in the carbonyl index by 16% was observed following inoculation with B. cereus. The metabolic activity of this strain contributed to the reduction of the film’s tensile strength by 17% and to the increase in the permeability to O2 and CO2. The most intense degradation of PET film was observed in the presence of bacteria and plants. B. subtilis strain combined with miscanthus plantings may be a promising method for accelerating PLA and PET degradation in compost soil.

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“…S. plymuthica used in this study is characterized by high metabolic activity; it is able to biodegrade plastic in compost and agricultural soil and stimulate the growth of B. napus , Miscanthus x giganteus , and Salix viminalis [ 70 , 71 ]. It was shown that several salt-tolerant strains of S. plymuthica improved cucumber biomass and yield via synthesis of IAA [ 72 ].…”

Section: Resultsmentioning

confidence: 99%

In Silico Study of the RSH (RelA/SpoT Homologs) Gene Family and Expression Analysis in Response to PGPR Bacteria and Salinity in Brassica napus

Dąbrowska

Turkan

Tylman-Mojżeszek

et al. 2021

IJMS

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Among several mechanisms involved in the plant stress response, synthesis of guanosine tetra and pentaphosphates (alarmones), homologous to the bacterial stringent response, is of crucial importance. Plant alarmones affect, among others, photosynthetic activity, metabolite accumulation, and nutrient remobilization, and thus regulate plant growth and development. The plant RSH (RelA/SpoT homolog) genes, that encode synthetases and/or hydrolases of alarmones, have been characterized in a limited number of plant species, e.g., Arabidopsis thaliana, Oryza sativa, and Ipomoea nil. Here, we used dry-to-wet laboratory research approaches to characterize RSH family genes in the polyploid plant Brassica napus. There are 12 RSH genes in the genome of rapeseed that belong to four types of RSH genes: 6 RSH1, 2 RSH2, 3 RSH3, and 1 CRSH. BnRSH genes contain 13–24 introns in RSH1, 2–6 introns in RSH2, 1–6 introns in RSH3, and 2–3 introns in the CRSH genes. In the promoter regions of the RSH genes, we showed the presence of regulatory elements of the response to light, plant hormones, plant development, and abiotic and biotic stresses. The wet-lab analysis showed that expression of BnRSH genes is generally not significantly affected by salt stress, but that the presence of PGPR bacteria, mostly of Serratia sp., increased the expression of BnRSH significantly. The obtained results show that BnRSH genes are differently affected by biotic and abiotic factors, which indicates their different functions in plants.

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Biodegradation of the plastics PLA and PET in cultivated soil with the participation of microorganisms and plants

Cited by 78 publications

References 38 publications

Transcriptome-Guided Insights Into Plastic Degradation by the Marine Bacterium

Transcriptome-Guided Insights Into Plastic Degradation by the Marine Bacterium

Combined use of Bacillus strains and Miscanthus for accelerating biodegradation of poly(lactic acid) and poly(ethylene terephthalate)

In Silico Study of the RSH (RelA/SpoT Homologs) Gene Family and Expression Analysis in Response to PGPR Bacteria and Salinity in Brassica napus

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