Biodegradation of pyrene by a Pseudomonas aeruginosa strain RS1 isolated from refinery sludge

Biosurfactant-enhanced bioremediation of aged polycyclic aromatic hydrocarbons (PAHs) in creosote contaminated soil

“…The PAH fraction in the liquid phase was extracted using the method described by Ghosh et al (2014). Briefly, 30 mL supernatant was decanted in to 150 mL bottle after centrifugation and extracted twice with 30 mL n-hexane.…”

“…The cultures were incubated at 37 C and shaker speed of 120 rpm for 16 days. Periodically the residual PAH was sacrificially extracted using the method described by Ghosh et al (2014). Briefly, the entire content of a set of flasks in duplicate were extracted twice with n-hexane (extraction ratio 1:1).…”

Isolation and characterization of glycolipid biosurfactant produced by a Pseudomonas otitidis strain isolated from Chirimiri coal mines, India

“…Pyrene (a four-ring PAHs) is one of the most abundant high-molecular weight PAHs present in the environment and due to its low bioavailability recalcitrant property, removal from the environment is a challenging task. Degradation of pyrene or other PAHs occurs mostly by microbial means and many bacterial species have been involved in this process (Zhong et al 2011;Kumari et al 2013;Ma et al 2013;Ghosh et al 2014).…”

Isolation and characterization of bacterial strains with pyrene metabolic functions from cow dung and Terminalia catappa phylloplane

“…They are produced and released into the environment through natural and anthropogenic pyrolysis of organic material such as forest fires, automobile exhaust, coal-refining processes and activities of the petroleum industry. Owing to their genotoxicity, carcinogenicity, mutagenicity and recalcitrance to microbial attack (Ghosh et al 2014;Moscoso et al 2012;Cerniglia, 1992), most PAHs, particularly high molecular weight (HMW) PAHs, are of great environmental and public health concern. As a consequence, many of the HMW PAHs are listed as priority pollutants by USEPA (Keith and Telliard, 1979;Wang et al 2000) and also by environmental protection agencies of most countries including Nigeria.…”