Biodegradation of pretreated pig bristles by Bacillus cereus B5esz

Łaba, Wojciech; Kopeć, Wiesław; Chorążyk, Dorota; Kancelista, Anna; Piegza, Michał; Malik, Kinga

doi:10.1016/j.ibiod.2015.02.024

Cited by 20 publications

(15 citation statements)

References 34 publications

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“…Another method used to dissolve keratins is a combination of enzymatic and chemical treatment (Mokrejs, Svoboda, Hrncirik, Janacova, & Vasek, ). Reports on application of thermo‐chemical treatment of keratins have appeared recently, however these methods occur in different experimental layout, aimed in aiding subsequent enzymatic digestion (Łaba et al, ).…”

Section: Solubilization Of Keratinsmentioning

confidence: 99%

Solubilization of keratins and functional properties of their isolates and hydrolysates

2018

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The keratinous wastes of the textile industry and poultry slaughterhouses may be used as sources of soluble keratins or hydrolysates. This review presents methods for processing raw keratin-based materials into bioproducts with functional and bioactive properties suitable for biomedical, cosmetic, food, and agricultural applications. Soluble keratin can be obtained by thermal treatment in some organic solvents, reduction, or oxidation of the disulfide bonds. Recent studies have shown that keratins contain amino acid sequences with high biological activities such as antioxidant, angiotensin I converting enzyme inhibitory, dipeptidyl peptidase IV inhibitory, and antimicrobial.Peptides containing these sequences may find numerous applications as value-added products in the food industry. More research devoted to development of methods for conversion of animal by-products to novel products is needed. Further technological investigations to create large-scale production methods are also necessary. Practical applicationsThe keratinous wastes represent a problematic by-product to the wool textile industry and poultry slaughterhouses due to the large volumes and their high pollutant load. They are usually incinerated or used for low value purposes such as fertilizers. This review focuses on the trends of application of keratin recovered from animal by-products. Biomaterials for regenerative medicine, cosmetic formulations, and biodegradable food packaging can be obtained as a result of keratin self-assembly. Several peptide sequences released by hydrolysis as bioactive peptides should be studied further for their in vivo antihypertensive, and antidiabetic effects, as well as functional ingredients in foods. K E Y W O R D Sbioactive peptides, functional properties, keratin hydrolysates, keratin isolates

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Section: Solubilization Of Keratinsmentioning

confidence: 99%

Solubilization of keratins and functional properties of their isolates and hydrolysates

2018

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“…Keratinolytic bacteria Bacillus cereus PCM 2849 and B. subtilis PCM 2850, formerly referred to as B. cereus B5esz and B. subtilis P22, respectively, were applied in the study . The strains were deposited in the Polish Collection of Microorganisms at the Institute of Immunology and Experimental Therapy in Wroclaw, Poland.…”

Section: Methodsmentioning

confidence: 99%

“…Proteolytic (or keratinolytic) activity of the preparation was tested, as described above, on a variety of soluble and insoluble substrates (1% w/v): casein (VWR), hemoglobin soluble (Sigma–Aldrich), collagen type I (Sigma–Aldrich), elastin (Sigma–Aldrich), chicken feathers (washed and degreased), pretreated chicken feathers, pig bristles (washed and degreased), pretreated pig bristles. The thermo‐chemical pretreatment of keratinous substrates was performed as described previously, by autoclaving feathers and bristles in 10 mmol L −1 or 100 mmol L −1 solution of sodium sulfite, respectively, under standard conditions (20 min, 121°C). One unit of proteolytic (or keratinolytic) activity was expressed as 1 µmol of released tyrosine calculated per 1 mg of protein within 1 h.…”

Section: Methodsmentioning

confidence: 99%

Evaluation of brewer's spent grain as a substrate for production of hydrolytic enzymes by keratinolytic bacteria

Łaba

Piegza

Kawa-Rygielska

2016

J of Chemical Tech & Biotech

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BACKGROUND Brewer's spent grain (BSG) is the most abundant by‐product of the brewery industry. The current massive output of BSG requires novel methods of bioconversion into added‐value products. Its composition and high biological value, i.e. high protein content, allows for various biotechnological applications. RESULTS Here, BSG was used as a substrate for submerged cultivation of keratinolytic bacteria, Bacillus cereus PCM 2849 and B. subtilis PCM 2850. The strains biosynthesized proteases to decompose proteins within the substrate and amylases to degrade residual starch. Enzymes degrading structural polysaccharides were also present. Bacillus cereus produced multiple proteases with high activity, predominantly with molecular weight (mw) >70 kDa, depending on the presence of minerals. Bacillus subtilis biosynthesised proteases of wide mw range, independently of the mineral components. Crude culture fluids of both bacteria exhibited proteolytic activity on a variety of proteins, including keratins. Structural deterioration of the BSG in microbial cultures was observed. CONCLUSION The BSG was a suitable medium component for the production of bacterial proteases and polysaccharide‐degrading enzymes. Two keratinolytic Bacilli, grown in the presence of BSG utilized residual proteins and biosynthesized proteases exhibiting keratinolytic activity. Bacillus cereus dominated in terms of protease production, while B. subtilis presented higher production of amylases, cellulases and xylanases. © 2016 Society of Chemical Industry

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“…5% wt. 5,34,35 Pig bristles started to decompose upon microwave irradiation at high temperature and sulphur, mainly in the form of ions, was released. Copper sulfide was then formed by combining S 2with Cu + and particles were selfaggregated to minimize the surface energy.…”

Section: Scheme 1 Reaction Scheme For the Synthesis Of Pb-cu2smentioning

confidence: 99%

“…for the high content of keratin). [4][5][6][7] Herein we report an unprecedented simple route for the valorization of pig bristles for the production of a high performance material as photocatalyst. Specifically, the synthesis of Cu2S carbon nanocomposite (denoted as pb-Cu2S, where "pb" states for "pig bristle derived") was accomplished using pig bristles as sulphur and carbon source.…”

Section: Introductionmentioning

confidence: 99%

Microwave-assisted valorization of pig bristles: towards visible light photocatalytic chalcocite composites

2018

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Waste valorization for the production of valuable materials is of great importance for a sustainable development. Herein, a new green methodology for the synthesis of photocatalytically active copper sulfide (Cu2S) carbon composites using pig bristles is reported. The catalyst was prepared via microwave-assisted methodology using ethylene glycol as solvent, pig bristles as sulfur and carbon source, and copper chloride as metal precursor. Cu2S carbon composites (denoted as pb-Cu2S, where "pb" states for "pig bristle") were characterized by XRD, N2 physisorption, EDX and UV-Vis spectroscopy. In order to validate the practical utilization of pig bristles-derived chemicals , the photocatalytic degradation of methyl red using pb-Cu2S was investigated under white, blue, green and red visible light irradiation. Electronic Supplementary Information (ESI) available: The scheme of the photoreactor; the complete list of XPRD peaks; EDX mapping results; N2 adsorption−desorption isotherm of pb-Cu2S; See

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Biodegradation of pretreated pig bristles by Bacillus cereus B5esz

Cited by 20 publications

References 34 publications

Solubilization of keratins and functional properties of their isolates and hydrolysates

Solubilization of keratins and functional properties of their isolates and hydrolysates

Evaluation of brewer's spent grain as a substrate for production of hydrolytic enzymes by keratinolytic bacteria

Microwave-assisted valorization of pig bristles: towards visible light photocatalytic chalcocite composites

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