Biodegradation of nitrilotriacetate in activated sludge

Swisher, R. D.; Crutchfield, M. M.; Caldwell, D.

doi:10.1021/es60010a004

Cited by 65 publications

(12 citation statements)

References 2 publications

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“…The biodegradability of NTA in soils, freshwater, and sewage treatment systems (16,17,19) has been firmly established. This study on the fate of NTA in estuarine waters demonstrates the low probability of biodegradation of this compound in saline waters.…”

Section: Discussionmentioning

confidence: 99%

“…To properly assess the possible hazards of large-scale usage of NTA, its biological and chemical fate in various environments must be determined. Numerous to be biodegradable in activated-sludge systems (14,16), river water (17), soils (18,19), and seawater (6). Except for the last study, in which no residue analyses were performed, no metabolic intermediate accumulated in any environment studied.…”

mentioning

confidence: 99%

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Distribution of bacteria with nitrilotriacetate-degrading potential in an estuarine environment

Bourquin¹,

Przybyszewski²

1977

Appl Environ Microbiol

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MATERIALS AND METHODS Chemicals. NTA, the sodium salts of NTA, Bi(NO3)3, POPOP {1,4-bis-[2]-(5-phenyloxazolyl)-benzene}, and PPO (2,5-diphenyloxazole) were obtained from Eastman Organic Chemical Co., Rochester, N.Y. Mercury (triple distilled) was purchased from Bethlehem Apparatus Co., Hellerton, Pa. Radiochemicals were purchased from Amersham/ Searle Corp., Arlington Heights, Ill. Growth media. Heterotrophic bacteria were enumerated on Zobell 2216 marine medium (Difco, Detroit, Mich.). The salinity in all media was adjusted to that of the sampling site (5 to 28%.) by addition of NaCl. Salinity for this report is a measure of "salt" concentration commonly expressed as parts per thousand (%. or ppt); artificial seawater is adjusted to the proper salinity by addition of NaCl to give equivalent salinity to environmental sam-411

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Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

Distribution of bacteria with nitrilotriacetate-degrading potential in an estuarine environment

Bourquin¹,

Przybyszewski²

1977

Appl Environ Microbiol

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“…in a 1:1 molar ratio (Swisher et al 1967), and this was predicted by MINEQL+ when it was used to model the speciation of Cu and NTA in phosphate-buffered medium. Figure 2 compares the experimental results for degradation of 2 mM NTA with the predicted speciation of NTA as the copper concentration was increased.…”

Section: Coppermentioning

confidence: 99%

Effect of metal complexation on the bioavailability of nitrilotriacetic acid to Chelatobacter heintzii ATCC 29600

White¹,

Knowles²

2000

Arch. Microbiol.

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Many polluted sites contain a mixture of organics and heavy metals. Nitrilotriacetic acid has been chosen as a model organic compound to study the effect of metal binding on organic bioavailability and degradation of organics. The effect of varying the ratio of metal to nitrilotriacetic acid on its utilisation has been examined using the gram-negative bacterium Chelatobacter heintzii ATCC 29600. The following parameters of substrate utilisation were examined: growth, degradation, respiration, mineralisation and nitrilotriacetic acid uptake. Complexation of nitrilotriacetic acid by Cu(II), Ni(II), Co(II) and Zn(II) prevented utilisation of nitrilotriacetic acid by C. heintzii; complexation to Fe(III) or Mn(II) did not. The pattern of inhibition was consistent with a 1:1 stoichiometry of metal binding to nitrilotriacetic acid. Inhibition was not due to metal ion toxicity, but was a result of metal-nitrilotriacetic acid complexes being recalcitrant to degradation. In addition, the effect of complexing (phosphate) and non-complexing (PIPES) buffers on bioavailability was examined: Co and Zn prevented degradation of nitrilotriacetic acid in PIPES buffer, but not in phosphate buffer. This was due to the removal of Co and Zn from solution by phosphate precipitation, leaving nitrilotriacetic acid uncomplexed. The results demonstrated that metal-organic complexation can alter the bioavailability of organic pollutants and may also modulate the toxicity of heavy metals.

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“…Some work has been conducted on how the complexed metal influences chelate degradation (mainly NTA) in soils (Tiedje and Mason 1974); sediments (Bolton et al 1993); waters (Swisher et al 1974); sewage (Madsen and Alexander 1985;Swisher et al 1967); and with microorganisms (Firestone and Tiedje 1975;Madsen and Alexander 1985). A study of DVA, EDTA, and NTA mineralization in terrestrial subsurface sediments (Bolton et al 1993) has demonstrated through aqueous speciation modeling ) that the form of the chelate in solution varied among the surface soil and subsurface sediments.…”

Section: Nuregicr-6124mentioning

confidence: 99%

Characterization of radionuclide-chelating agent complexes found in low-level radioactive decontamination waste. Literature review

Serne¹,

Felmy²,

Cantrell³

et al. 1996

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DISCLAIMERNUREGER-6124 is not a substitute for NRC regulations and compliance is not required. The approaches andor methods described in this NUREG/CR are provided for information only. Publication of this report does not necessarily constitute NRC approval or agreement with the information contained herein. AbstractUnder the regulations outlined in 10 CFR 61, the U.S. Nuclear Regulatory Commission is responsible for regulating the safe land disposal of low-level radioactive wastes that may contain organic chelating agents. Such agents include ethylenediaminetetraacetic acid (EDTA), diethylenetriaminepentaacetic acid (DTPA), . picolinic acid, oxalic acid, and citric acid, and can form radionuclide-chelate complexes that may enhance the migration of radionuclides from disposal sites.Data from the available literature indicate that chelates, most notably picolinate and EDTA, can leach from solidified decontamination wastes in moderate concentration (i.e., 1-100 ppm) and can potentially complex certain radionuclides in the leachates. The effects of the formation of such radionuclide-chelate complexes on the migration of radionuclides in groundwater systems is still difficult to quantitatively predict owing to the dependence of such migration on several factors including the chemical composition of the groundwater, the specific adsorbing surfaces present in the sods, and the ability of microorganisms to biodegrade the chelates. However, in general it appears that both EDTA and DTPA have the potential to mobilize radionuclides from waste disposal sites because such chelates can leach in moderate concentration, form strong radionuclide-chelate complexes, and can be recalcitrant to biodegradation. It also appears that oxalic acid and citric acid will not greatly enhance the mobility of radionuclides from waste disposal sites because these chelates do not appear to leach in high concentration, tend to form relatively weak radionuclide-chelate complexes, and can be readily biodegraded. In the case of picolinic acid, insufficient data are available on adsorption, complexation of key radionuclides (such as the actinides), and biodegradation to make definitive predictions, although the available data indicate that picolinic acid can chelate certain radionuclides in the leachates. Contents 3.12Mass spectra of (a) Cu(EDTA) and (b) Cu(HEDTA I Executive SummaryA variety of chemical decontamination processes are used to remove the build-up of radioactive-activated metals and corrosion products from the cooling systems of nuclear power plants. AU of these decontamination processes use chelating agents, such as ethylenediaminetetraaceec acid (EDTA), picolinic acid, oxalic acid, citric acid, and less frequently, diethylenetriaminepentaacetic acid (DTPA), to complex the released radionuclides. The complexed radionuclides and any excess uncomplexed chelates are then removed onto cation-or anion-exchange resins. The U.S. Nuclear Regulatory Commission (NRC), as defined in 10 CFR 61, is responsible for regulating the disposal of suc...

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Biodegradation of nitrilotriacetate in activated sludge

Cited by 65 publications

References 2 publications

Distribution of bacteria with nitrilotriacetate-degrading potential in an estuarine environment

Distribution of bacteria with nitrilotriacetate-degrading potential in an estuarine environment

Effect of metal complexation on the bioavailability of nitrilotriacetic acid to Chelatobacter heintzii ATCC 29600

Characterization of radionuclide-chelating agent complexes found in low-level radioactive decontamination waste. Literature review

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