Biodegradation of mixed polycyclic aromatic hydrocarbons by <i>Pseudomonas</i> sp. isolated from estuarine sediment

Bhatawadekar, Vasudha C.; Damare, Samir; Garg, Anita

doi:10.1080/10889868.2021.1993779

Cited by 8 publications

(1 citation statement)

References 32 publications

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“…Pseudomonas oleovorans , as a cold-tolerant and amphotrophic basophilic bacterium capable of utilizing hydrocarbons, can grow at 4–42 °C, with the optimum growth at 35 °C [ 16 ]. Previous studies showed that Pseudomonas oleovorans , which was isolated from estuarine sediment, can degrade polycyclic aromatic hydrocarbons [ 17 , 18 ]. A series of aerobic batch degradation experiments showed that Pseudomonas oleovorans can biodegrade tetrahydrofuran, benzene, toluene, and ethylbenzene [ 19 , 20 , 21 ].…”

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confidence: 99%

Isolation of Pseudomonas oleovorans Carrying Multidrug Resistance Proteins MdtA and MdtB from Wastewater

et al. 2023

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The pollution of industrial wastewater has become a global issue in terms of economic development and ecological protection. Pseudomonas oleovorans has been studied as a bacterium involved in the treatment of petroleum pollutants. Our study aimed to investigate the physicochemical properties and drug resistance of Pseudomonas oleovorans isolated from industrial wastewater with a high concentration of sulfate compounds. Firstly, Pseudomonas oleovorans was isolated and then identified using matrix-assisted flight mass spectrometry and 16S rDNA sequencing. Then, biochemical and antibiotic resistance analyses were performed on the Pseudomonas oleovorans, and a microbial high-throughput growth detector was used to assess the growth of the strain. Finally, PCR and proteomics analyses were conducted to determine drug-resistance-related genes/proteins. Based on the results of the spectrum diagram and sequencing, the isolated bacteria were identified as Pseudomonas oleovorans and were positive to reactions of ADH, MTE, CIT, MLT, ONPG, and ACE. Pseudomonas oleovorans was sensitive to most of the tested antibiotics, and its resistance to SXT and CHL and MIN and TIM was intermediate. The growth experiment showed that Pseudomonas oleovorans had a good growth rate in nutrient broth. Additionally, gyrB was the resistance gene, and mdtA2, mdtA3, mdtB2, mdaB, and emrK1 were the proteins that were closely associated with the drug resistance of Pseudomonas oleovorans. Our results show the biochemical properties of Pseudomonas oleovorans from industrial wastewater with a high concentration of sulfate compounds and provide a new perspective for Pseudomonas oleovorans to participate in biological removal of chemical pollutants in industrial wastewater.

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