Pseudomonas in environmental bioremediation of hydrocarbons and phenolic compounds- key catabolic degradation enzymes and new analytical platforms for comprehensive investigation

Medić, Ana; Karadžić, Ivanka

doi:10.1007/s11274-022-03349-7

Cited by 24 publications

(9 citation statements)

References 177 publications

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“…Anaerobic microbes like sulfate-reducing bacteria, methanogens, and acetogens utilise a variety of reaction enzymes and metabolic pathways to carry out successive hydrocarbon breakdowns under oxygen-deficient conditions (Koh, L. M., et al, 2023). These converging pathways include fermentative processes, syntrophic associations, and the production of methane, hydrogen, or some other reduced compound as a metabolic by-product (Medić, A. B., et al, 2022).…”

Section: Aerobic and Anaerobic Degradation Pathwaysmentioning

confidence: 99%

“…Enzymatic systems are typically the main actors in the petroleum hydrocarbon biodegradation process, where the hydrocarbon molecules are converted into simpler constituents. These enzymes are produced by different microorganisms, including bacteria, archaea, and fungi, whose crewed biochemical reactions are assisted through a step-by-step degradation process (Medić, A. B., et al, 2022).…”

Section: Enzymatic Mechanisms Involvedmentioning

confidence: 99%

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Petroleum Hydrocarbons Biodegradation Uncovering the Variety and Capabilities of Oil-Oxidizing Microbes

Hassand,

Omirbekova,

Baseer

et al. 2024

ejtas

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The biodegradation of petroleum hydrocarbons is a valuable process used to reduce the ecological influences of oil spills and pollution. This comprehensive review immerses readers in the sophisticated universe of oil-oxidizing organisms, the diversity and functionality of which are unveiled. By examining different bacterial groups, such as aerobic and anaerobic bacteria, fungi, archaea, and algae, the study shows enzymatic and metabolic processes exploited during biodegradation. Environmental factors, substrate characteristics, and microbial interactions are the main determinants that contribute to the good performance of the biodegradation of petroleum hydrocarbons. The effectiveness of various biotechnological strategies like in-situ and ex-situ bioremediation, bioaugmentation, and bio stimulation is being tested to determine their ability to embrace the microbial capabilities for environmental restoration.

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Section: Aerobic and Anaerobic Degradation Pathwaysmentioning

confidence: 99%

Section: Enzymatic Mechanisms Involvedmentioning

confidence: 99%

Petroleum Hydrocarbons Biodegradation Uncovering the Variety and Capabilities of Oil-Oxidizing Microbes

Hassand,

Omirbekova,

Baseer

et al. 2024

ejtas

View full text Add to dashboard Cite

show abstract

“…The bacterium employs an array of enzymes, including hydroxylases, dioxygenases, and dehydrogenases, which play roles in the initial steps of hydrocarbon degradation [ 15 ]. These enzymes enable the breaking down of complex hydrocarbon structures into metabolically usable intermediates, facilitating their incorporation into central metabolic pathways [ 16 ]. Plasmid-mediated resistance genes carried on plasmids can be transferred between Gram-negative bacteria, facilitating the spread of biocide resistance.…”

Section: Introductionmentioning

confidence: 99%

Metabolic Changes in Pseudomonas oleovorans Isolated from Contaminated Construction Material Exposed to Varied Biocide Treatments

Ali,

Ferrieres,

Jass

et al. 2024

Metabolites

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Biocide resistance poses a significant challenge in industrial processes, with bacteria like Pseudomonas oleovorans exhibiting intrinsic resistance to traditional antimicrobial agents. In this study, the impact of biocide exposure on the metabolome of two P. oleovorans strains, namely, P. oleovorans P4A, isolated from contaminated coating material, and P. oleovorans 1045 reference strain, were investigated. The strains were exposed to 2-Methylisothiazol-3(2H)-one (MI) MIT, 1,2-Benzisothiazol-3(2H)-one (BIT), and 5-chloro-2-methyl-isothiazol-3-one (CMIT) at two different sub-inhibitory concentrations and the lipids and polar and semipolar metabolites were analyzed by ultra-high performance liquid chromatography quadrupole time-of-flight mass spectrometry UPLC–Q–TOF/MS. Exposure to the BIT biocide induced significant metabolic modifications in P. oleovorans. Notable changes were observed in lipid and metabolite profiles, particularly in phospholipids, amino acid metabolism, and pathways related to stress response and adaptation. The 1045 strain showed more pronounced metabolic alterations than the P4A strain, suggesting potential implications for lipid, amino acid metabolism, energy metabolism, and stress adaptation. Improving our understanding of how different substances interact with bacteria is crucial for making antimicrobial chemicals more effective and addressing the challenges of resistance. We observed that different biocides trigged significantly different metabolic responses in these strains. Our study shows that metabolomics can be used as a tool for the investigation of metabolic mechanisms underlying biocide resistance, and thus in the development of targeted biocides. This in turn can have implications in combating biocide resistance in bacteria such as P. oleovorans.

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“…Oxygenases are usually enzymatic complexes that use cofactors and carry out redox reactions using oxygen as a co-reagent and NADH or iron as an electron donor 16 . The aromatic compounds degradation pathway in bacteria can be generally divided in two steps, indicated as upper and lower pathways 17 . The reactions of the upper pathways involve the activation of the aromatic ring by addition of one or two hydroxyl groups on two adjacent carbon atoms, often catalyzed by monooxygenases.…”

Section: Introductionmentioning

confidence: 99%

Bioconversion of 4-hydroxyestradiol by extradiol ring-cleavage dioxygenases from Novosphingobium sp. PP1Y

Mensitieri

Bosso

Piaz

et al. 2023

Sci Rep

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Livestock breeding activities and pharmaceutical wastes lead to considerable accumulation of steroid hormones and estrogens in wastewaters. Here estrogens act as pro-cancerogenic agents and endocrine disruptors interfering with the sexual development of aquatic animals and having toxic effects in humans. Environmental bacteria play a vital role in estrogens degradation. Their wide reservoir of enzymes, such as ring cleavage dioxygenases (RCDs), can degrade the steroid nucleus, catalyzing the meta-cleavage of A, B or D steroid rings. In this work, 4 extra-diol ring cleavage dioxygenases (ERCDs), PP28735, PP26077, PP00124 and PP00193, were isolated from the marine sphingomonad Novosphingobium sp. PP1Y and characterized. Enzymes kinetic parameters were determined on different synthetic catecholic substrates. Then, the bioconversion of catechol estrogens was evaluated. PP00124 showed to be an efficient catalyst for the degradation of 4-hydroxyestradiol (4-OHE2), a carcinogenic hydroxylated derivate of E2. 4-OHE2 complete cleavage was obtained using PP00124 both in soluble form and in whole recombinant E. coli cells. LC–MS/MS analyses confirmed the generation of a semialdehyde product, through A-ring meta cleavage. To the best of our knowledge, PP00124 is the first characterized enzyme able to directly degrade 4-OHE2 via meta cleavage. Moreover, the complete 4-OHE2 biodegradation using recombinant whole cells highlighted advantages for bioremediation purposes.

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Pseudomonas in environmental bioremediation of hydrocarbons and phenolic compounds- key catabolic degradation enzymes and new analytical platforms for comprehensive investigation

Cited by 24 publications

References 177 publications

Petroleum Hydrocarbons Biodegradation Uncovering the Variety and Capabilities of Oil-Oxidizing Microbes

Petroleum Hydrocarbons Biodegradation Uncovering the Variety and Capabilities of Oil-Oxidizing Microbes

Metabolic Changes in Pseudomonas oleovorans Isolated from Contaminated Construction Material Exposed to Varied Biocide Treatments

Bioconversion of 4-hydroxyestradiol by extradiol ring-cleavage dioxygenases from Novosphingobium sp. PP1Y

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