Biodegradation of Metal-EDTA Complexes by an Enriched Microbial Population

Thomas, R. A. P.; Lawlor, K.; Bailey, Mark; Macaskie, Lynne E.

doi:10.1128/aem.64.4.1319-1322.1998

Cited by 80 publications

(25 citation statements)

References 30 publications

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“…Sterile technique was used, although none of the Synechococcus and Prochlorococcus isolates used were axenic (Table 1). Marine bacteria can produce copper-binding compounds (Schreiber et al 1990) and can also degrade metal chelator complexes (Thomas et al 1998), so this was some cause for concern. By the end of the study, we had finally obtained an axenic culture of Prochlorococcus (Saito 2000), so we were able to do a control experiment to examine the effects of bacteria on our results.…”

Section: Methodsmentioning

confidence: 99%

Copper toxicity and cyanobacteria ecology in the Sargasso Sea

Mann

Ahlgren

Moffett

et al. 2002

Limnology & Oceanography

206

157

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The closely related cyanobacteria Synechococcus and Prochlorococcus have different distributions in stratified water columns in the northern Sargasso Sea. The abundance of Synechococcus is relatively uniform with depth, but Prochlorococcus cell numbers are low within shallow mixed layers and high in and below the thermocline. Because free cupric ion (free Cu 2ϩ ) concentrations are high (up to 6 pM) in shallow mixed layers and lower in deeper water, there is an inverse relationship between Prochlorococcus densities and the free Cu 2ϩ concentration. We explored the possibility of a causal underpinning for this relationship by examining the relative copper sensitivities of Prochlorococcus and Synechococcus in cultures and field populations. Prochlorococcus isolates from both the high-and low-light adapted ecotypes were inhibited at free Cu 2ϩ concentrations that had no effect on Synechococcus. However, the high-light adapted strains were more copper resistant than their low-light adapted counterparts. When copper was added to Prochlorococcus from environments where the in situ free Cu 2ϩ was low (in deeply mixed water columns and below the mixed layer in stratified conditions), net growth rates were substantially reduced and cells arrested in the G 1 and early S phases of the cell cycle. Prochlorococcus in shallow mixed layers were less sensitive to copper and were probably members of the copper-resistant high-light adapted ecotype. Synechococcus were relatively copper resistant across a range of environments in the Sargasso Sea. These observations are consistent with our hypothesis that copper plays a role in cyanobacteria ecology in the Sargasso Sea.Human activities have produced a measurable increase in the concentrations of trace metals in even remote environments. For instance, the atmospheric flux of copper to Greenland ice has increased by over an order of magnitude from the pre-Roman period to the present (Hong et al. 1996). 3 Corresponding author (chisholm@mit.edu). AcknowledgmentsWe thank P. Lam and H. Hsu for invaluable technical assistance in the laboratory and M. Saito for stimulating discussions. We also thank the captain and crew of the RV Oceanus for making the field experiments possible. Comments from two anonymous reviewers have been very helpful in revising the manuscript.

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Section: Methodsmentioning

confidence: 99%

Copper toxicity and cyanobacteria ecology in the Sargasso Sea

Mann

Ahlgren

Moffett

et al. 2002

Limnology & Oceanography

206

157

View full text Add to dashboard Cite

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“…They had no illusions regarding the difficulty of treating these wastes [208]. In this expos e, Maszenan et al [208] cited the work of Bolton et al [209] and Thomas et al [210], showing that the practice and understanding of biodegradation did not significantly improve between 1996 and 2011.…”

Section: Metal Carboxylate-chelated Complexesmentioning

confidence: 98%

“…Games and Staubach [38] attempted to compare the rates of ozonation of unbound and chelated nitroloacetic acid (NTA, of Mg, Cu, and Zn); however, the results were inconclusive. Morooka et al [39] Biodegradation [198][199][200][201][202][203][204][205][206][207][208][209][210][211][212][213][214] Hybrid treatment methods [215][216][217][218][219][220][221] ozonolysed the EDTA chelates of Mg, Zn, and Cu and found that the rate of decomposition was in the following order: Mg > Zn > Cu. Gilbert and Hoffmann-Glewe [40] also compared the rates of ozonolysis of 1:1 EDTA chelates, studying the rate of degradation of EDTA (10 23 mol dm 23 ) by ozone in the presence of metallic ions.…”

Section: Ozonolysismentioning

confidence: 99%

Separation of transition metals and chelated complexes in wastewaters

Cheung

2014

Env Prog and Sustain Energy

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This review responds to the ongoing needs of wastewater engineers tasked with treating aqueous solutions containing chelated complexes of metal ions, of which nickel citrate in electrodeless plating and copper–EDTA in electronic chip board manufacturing are two key examples. Because of the presence of these sequestering agents, metallic ions cannot be readily precipitated by alkalinity, making a compelling case for the discovery of alternative methods of treatment. This review is a critical appraisal of the varying degrees of success in separation process strategies deployed for the recovery of metallic ions under such challenging chemical conditions. Guidance is provided on making progress toward satisfactory industrial solutions to surmount these difficulties. © 2014 American Institute of Chemical Engineers Environ Prog, 34: 761–783, 2015

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“…that unde¢ned, rather complex systems were used. In such systems, EDTA was found to be degraded by mixed microbial consortia when added in the free form or as chelate of Cu 2 , Cd 2 , Zn 2 , Ni 2 , Mn 2 , Ca 2 or Fe 3 [149,153].…”

Section: Edtamentioning

confidence: 99%

Environmental fate and microbial degradation of aminopolycarboxylic acids

Bucheli-Witschel¹

2001

FEMS Microbiology Reviews

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Aminopolycarboxylic acids (APCAs) have the ability to form stable, water-soluble complexes with di- and trivalent metal ions. For that reason, synthetic APCAs are used in a broad range of domestic products and industrial applications to control solubility and precipitation of metal ions. Because most of these applications are water-based, APCAs are disposed of in wastewater and reach thus sewage treatment plants and the environment, where they undergo abiotic and/or biotic degradation processes. Recently, also natural APCAs have been described which are produced by plants or micro-organisms and are involved in the metal uptake by these organisms. For the two most widely used APCAs, nitrilotriacetate (NTA) and ethylenediaminetetraacetate (EDTA), transformation and mineralisation processes have been studied rather well, while for other xenobiotic APCAs and for the naturally occurring APCAs little is known on their fate in the environment. Whereas NTA is mainly degraded by bacteria under both oxic and anoxic conditions, biodegradation is apparently of minor importance for the environmental fate of EDTA. Photodegradation of iron(III)-complexed EDTA is supposed to be mostly responsible for its elimination. Isolation of a number of NTA- and EDTA-utilising bacterial strains has been reported and the spectrum of APCAs utilised by the different isolates indicates that some of them are able to utilise a range of different APCAs whereas others seem to be restricted to one compound. The two best characterised obligately aerobic NTA-utilising genera (Chelatobacter and Chelatococcus) are members of the alpha-subgroup of Proteobacteria. There is good evidence that they are present in fairly high numbers in surface waters, soils and sewage treatment plants. The key enzymes involved in NTA degradation in Chelatobacter and Chelatococcus have been isolated and characterised. The two first catabolic steps are catalysed by a monooxygenase (NTA MO) and a membrane-bound iminodiacetate dehydrogenase. NTA MO has been cloned and sequenced and its regulation as a function of growth conditions has been studied. Under denitrifying conditions, NTA catabolism is catalysed by a NTA dehydrogenase. EDTA breakdown was found to be initiated by a MO also which shares many characteristics with NTA MO from strictly aerobic NTA-degrading bacteria. In contrast, degradation of [S,S]-ethylenediaminedisuccinate ([S,S]-EDDS), a structural isomer of EDTA, was shown to be catalysed by an EDDS lyase in both an EDTA degrader and in a NTA-utilising Chelatococcus strain. So far, transport of APCAs into cells has only been studied for EDTA and the results obtained give strong evidence for an energy-dependent carrier system and Ca(2+) seems to be co-transported with EDTA. Due to their metal-complexing capacities, APCAs occur in the environment mostly in the metal-complexed form. Hence, the influence of metal speciation on various degradation processes is of utmost importance to understand the environmental behaviour of these compounds. In case of biodegradation, th...

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Biodegradation of Metal-EDTA Complexes by an Enriched Microbial Population

Cited by 80 publications

References 30 publications

Copper toxicity and cyanobacteria ecology in the Sargasso Sea

Copper toxicity and cyanobacteria ecology in the Sargasso Sea

Separation of transition metals and chelated complexes in wastewaters

Environmental fate and microbial degradation of aminopolycarboxylic acids

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