Biodegradation of long-chain n-paraffins from waste oil of car engine by Acinetobacter sp.

Koma, Daisuke; Hasumi, Fumihiko; Yamamoto, Etsuji; Ohta, Takao; Chung, Seon-Yong; Kubo, Michinori

doi:10.1016/s1389-1723(01)80120-1

Cited by 68 publications

(36 citation statements)

References 11 publications

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“…N-alkanes are widespread in nature and many microorganisms have capacity to use them as a carbon source. From petroleum polluted environments, hydrocarbon degrading microorganisms including yeasts (Ueda et al, 2000), algae (Ueno et al, 2002) and bacteria (Whyte et al, 1996;Koma et al, 2001;Wang et al, 2006) have been isolated or detected. It seems that distinct nalkane degrading microorganisms play roles in different habitats.…”

Section: Introductionmentioning

confidence: 99%

“…The degradation of LCAs is hindered by their limited bioavailability and they are hardly to be degraded by microorganisms in nature compared with short-chain hydrocarbons. In relation to the leaching of petroleum and the large amounts of waste lubricating oil, LCAs has the capability of causing serious environmental pollution all over the world (Koma et al, 2001). High level of LCAs has been detected in petroleum contaminated water bodies, sediments and soils.…”

Section: Introductionmentioning

confidence: 99%

“…The accumulation of LCAs in environments is harmful to ecosystems and to organisms (Salvayre et al, 1988;Baxter et al, 1991). Although other methods, such as incineration or dumping, could be used to treat the LCA wastes, these procedures adversely affect the environment (Koma et al, 2001). So, the investigation of microorganisms able to transform or degrade LCAs is essential to develop a new waste oil disposal system (Koma et al, 2001) or to clean the oil pollutants in the environment.…”

Section: Introductionmentioning

confidence: 99%

“…Although other methods, such as incineration or dumping, could be used to treat the LCA wastes, these procedures adversely affect the environment (Koma et al, 2001). So, the investigation of microorganisms able to transform or degrade LCAs is essential to develop a new waste oil disposal system (Koma et al, 2001) or to clean the oil pollutants in the environment. In another hand, the n-alkane degrading microorganisms are also bioresource to transform LCAs into pharmaceutical or industrial interested compounds (Ishige et al, 2002;Mikolasch et al, 2003;Sheu and Lee, 2004;Gao et al, 2005).…”

Section: Introductionmentioning

confidence: 99%

“…Up-to-date, only several Gram-positive bacteria or yeasts have been reported to be able to use solid paraffin, including Acinetobacter spp. (C 13 -C 44 ) (Sakai et al, 1994;Lai and Khanna, 1996;Koma et al, 2001), Arthrobacter nicotianae KCCB35 (C 10 to C 40 ) (Radwan et al, 1996), Rhodococcus sp. Q15 (C 12 to C 32 ) (Whyte et al, 1998), Candida spp.…”

Section: Introductionmentioning

confidence: 99%

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Oxidation of solid paraffin (C11−40) byPseudomonas aeruginosa MGP-1

et al. 2007

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Isolated from oil-contaminated soil, Pseudomonas aeruginosa strain MGP-1 was identified by 16S rDNA sequence analysis and phenotypic characterisation. This strain was highly competent in medium and in soils, grew and survived well under adverse conditions. MGP-1 did not produce the pathogenic factor pyocyanin and used paraffin (C 11-40 ) as sole carbon source. It degraded eicosane (C 20 ) more efficiently than other n-alkanes, removing 60% within 16 days. An alkB gene and intermediate metabolites of mono-alcohol and lipid acid corresponding to the oxidised paraffin were detected in the cell-free extracts of MGP-1, indicating that it possessed a mono-terminal oxidation. This is the first report to demonstrate that a Pseudomonas strain is able to degrade paraffin with more than 18 carbon atoms by mono-terminal oxidation. Considering all the results, MGP-1 could be designed as a soil-born, highly competent, long chain n-alkane degrading bacterium with low or perhaps without pathogenicity.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Oxidation of solid paraffin (C11−40) byPseudomonas aeruginosa MGP-1

et al. 2007

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Phenotypic Adaptations Help Rhodococcus erythropolis Cells during the Degradation of Paraffin Wax

Rodrigues

Carvalho

2019

Biotechnology Journal

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During crude oil extraction, the reduction in temperature and pressure results in the precipitation of paraffin wax that contains 20–40 carbon chain hydrocarbons. The paraffin wax may accumulate inside production tubes, pipelines, and processing facilities, and also in tankers during petroleum transportation. There are few bacterial strains that are able to degrade solid substrates. In the present study, the biodegradation of paraffin is evaluated using Rhodococcus erythropolis cells. This bacterium is able to grow using paraffin wax from an oil refinery plant as the sole carbon source. The cells grow as a thick biofilm over the solid substrate, make scale‐like structures that increase the area of the initially smooth surface of paraffin, produce biosurfactants, and become more negatively charged than ethanol‐ or glucose‐grown cells. When paraffin wax is supplied as microparticles, to increase the cell–substrate contact area and to simulate paraffin precipitation, the cells also adjust the composition of the fatty acids of the phospholipids of the cellular membrane to decrease its fluidity and paraffin biodegradation increases considerably. The study suggests that the phenotypic adaptation of R. erythropolis cells may be used to degrade paraffin wax under real conditions.

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Changes in toxicity during treatment of wastewater from oil plant contaminated with petroleum hydrocarbons

Steliga

Jakubowicz

Kapusta

2014

J. Chem. Technol. Biotechnol.

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BACKGROUND Exploitation of oil‐fields always faces problems with management of petroleum‐contaminated wastewaters. Wastewater, severely contaminated with petroleum hydrocarbons: total petroleum hydrocarbons (TPH, 340.7 mg L−1), benzene, toluene, ethylbenzene, xylene (BTEX, 5.52 mg L−1), phenols (2.95 mg L−1), and polycyclic aromatic hydrocarbons (PAH, 29.2 µg L−1), were pretreated on a flow‐line installation. The following processes were applied: flotation, coagulation with flocculation, sedimentation and filtration of sediments. Then a biological module with an active biopreparation (microbial consortium, based on non‐pathogenic indigenous bacteria and fungi) was used to remove extant hydrocarbons. RESULTS After wastewater pretreatment, TPH content decreased to 49.7 mg L−1. During biological treatment, petroleum contaminants (TPH, phenols, BTEX and PAH) were decreased by 89%, 69%, 62%, and 46%, respectively. Biodegradation reached 83–96% for nC11–nC22 and 34–68% for nC23–nC36 alkanes (more resistant). Microtox, MARA, Daphtoxkit and Ames test indicated that changes in toxicity were not strictly connected with the decrease in TPH contents, possibly due to the formation of toxic indirect metabolites during biodegradation. After treatment, wastewater had no toxic properties. CONCLUSION As results show, toxicological tests are an effective tool to monitor changes in toxicity during wastewaters treatment. This field scale study proved that combining physico‐chemical processes and biological treatment has high effectiveness in petroleum hydrocarbons removal. © 2014 Society of Chemical Industry

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Biodegradation of long-chain n-paraffins from waste oil of car engine by Acinetobacter sp.

Cited by 68 publications

References 11 publications

Oxidation of solid paraffin (C11−40) byPseudomonas aeruginosa MGP-1

Oxidation of solid paraffin (C11−40) byPseudomonas aeruginosa MGP-1

Phenotypic Adaptations Help Rhodococcus erythropolis Cells during the Degradation of Paraffin Wax

Changes in toxicity during treatment of wastewater from oil plant contaminated with petroleum hydrocarbons

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