Biodegradation of fuel oxygenates and their effect on the expression of a newly identified cytochrome P450 gene in Achromobacter xylosoxidans MCM2/2/1

Gunasekaran, Vijayalakshmi; Dönmez, Emre; Girhard, Marco; Urlacher, Vlada B.; Constantí, Magda

doi:10.1016/j.procbio.2013.09.028

Cited by 16 publications

(7 citation statements)

References 30 publications

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“…P. fluorescens and R. dairenensis also produced greater degradation of aromatics. A. xylosoxidans , which originated from marine environments, have been shown to be able to efficiently degrade a wide range of low/high molecular weight PAHs as well as many xenobiotic substrates including toluidine isomers, endosulfan, p‐toluenesulfonate, chlorocatechol, 2,4‐dichlorophenol, organophosphorous pesticide, etc . P. fluorescens has also been reported as the important genus of PAH utilizers .…”

Section: Resultsmentioning

confidence: 99%

“…A. xylosoxidans, which originated from marine environments, have been shown to be able to efficiently degrade a wide range of low/high molecular weight PAHs as well as many xenobiotic substrates including toluidine isomers, endosulfan, p-toluenesulfonate, chlorocatechol, 2,4-dichlorophenol, organophosphorous pesticide, etc. 30,31 P. fluorescens has also been reported as the important genus of PAH utilizers. 32,33 Cerqueira et al 6 demonstrated that B. megaterium strain BB06 could degrade 89.0% and 39.6% of aliphatic and aromatic fractions, respectively, in oily sludge.…”

Section: Oily Sludge Biodegradation In Biometer Flasksmentioning

confidence: 99%

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Enhanced bioremediation of oily sludge using co‐culture of specific bacterial and yeast strains

Xin-guo

Liu

2014

J of Chemical Tech & Biotech

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BACKGROUND Considerable volumes of petrochemical oily sludge can be generated from oil production and processing activities. Total petroleum hydrocarbons (TPH) contained in oily sludge are detrimental to human health and the environment. Thus, oily sludge is classified as a priority environmental pollutant by the US Environmental Protection Agency and it must be treated properly before discharge into the environment. RESULTS In this study, a functional microbial consortium consisting of Bacillus subtilis, Bacillus megaterium, Achromobacter xylosoxidans, Pseudomonas fluorescens, Candida tropicalis and Rhodotorula dairenensis, isolated from petroleum‐contaminated soil, was applied for bioremediation of petrochemical oily sludge in a shake flask and oily sludge microcosm study. These strains utilized the oily sludge as sole source of carbon and energy. The oily sludge contained 21.5% (w/w, dry weight basis) of total petrol hydrocarbons (TPH). Results showed that the microbial consortium exhibited an excellent oily sludge degradation capacity, reducing 92.5% of aliphatics, 79.3% of aromatics, 48.6% of NSO‐containing compounds, 31.8% of asphaltenes and 80.6% of TPH, by the end of the 1‐year microcosm experiment. In contrast, biodegradation rates of these fractions were 83.3%, 17.5%, 8.4%, 4.7% and 56.1%, respectively, in the non‐inoculated treatment. These strains survived well and adapted to the oil sludge environment throughout the incubation period. Dehydrogenase and polyphenoloxidase activity of the oily sludge was remarkably promoted by inoculation of the microbial consortium. CONCLUSION The results indicated that the microbial consortium has the potential to be used in bioremediation of petrochemical oily sludge polluted environments. © 2014 Society of Chemical Industry

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Section: Resultsmentioning

confidence: 99%

Section: Oily Sludge Biodegradation In Biometer Flasksmentioning

confidence: 99%

Enhanced bioremediation of oily sludge using co‐culture of specific bacterial and yeast strains

Xin-guo

Liu

2014

J of Chemical Tech & Biotech

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“…The separation of S. novella R8b extract (265.6 mg) yielded 14 different and homogeneous fractions. The main secondary metabolite of S. novella R8b was purified by the preparative TLC of fractions (2-3), (3-5)III and (6)(7)(8)(9)(10)(11)(12)(13)(14)d. The metabolite isolated showed chromatographic and spectroscopic properties (NMR and MS) similar to those reported in literature [26] for a compound known as maculosin (Fig 6).…”

Section: Bacterial Biocontrol Activitymentioning

confidence: 99%

“…Among these strategies, biodegradation processes are recognized as innovative, cost-effective and environmentally friendly options for the detoxification of MtBE-contaminated soil [4,7,8]. Some microorganisms can partially or completely degrade MtBE under aerobic or anaerobic conditions [9,10,11,12]. Hydrocarbon substrates in general are known to be selectively degraded by specific micro-organisms, consequently the use of microbial consortia can provide a broader spectrum of enzymes through co-metabolism [13].…”

Section: Introductionmentioning

confidence: 99%

Methyl t-butyl ether-degrading bacteria for bioremediation and biocontrol purposes

et al. 2020

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A total of fifteen potential methyl t-butyl ether (MtBE)-degrading bacterial strains were isolated from contaminated soil. They have been identified as belonging to the genera Bacillus, Pseudomonas, Kocuria, Janibacter, Starkeya, Bosea, Mycolicibacterium, and Rhodovarius. Bacillus aryabhattai R1B, S. novella R8b, and M. mucogenicum R8i were able to grow using MtBE as carbon source, exhibiting different growth behavior and contaminant degradation ability. Their biocontrol ability was tested against various fungal pathogens. Both S. novella R8b and B. aryabhattai were effective in reducing the development of necrotic areas on leaves within 48 hours from Botritys cinerea and Alternaria alternata inoculation. Whereas, M. mucogenicum effectively controlled B. cinerea after 72 hours. Similar results were achieved using Pythium ultimum, in which the application of isolated bacteria increased seed germination. Only M. mucogenicum elicited tomato plants resistance against B. cinerea. This is the first report describing the occurrence of bioremediation and biocontrol activities in M. mucogenicum, B. aryabhattai and S. novella species. The production of maculosin and its antibiotic activity against Rhizoctonia solani has been reported for first time from S. novella. Our results highlight the importance of multidisciplinary approaches to achieve a consistent selection of bacterial strains useful for plant protection and bioremediation purposes.

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“…Even though MTBE is recalcitrant to biodegradation due to its ether linkage and tertiary carbon, a number of microorganisms can either partially or completely degrade MTBE under aerobic or anaerobic conditions [17,18]. Only a few pure bacterial cultures can use MTBE as a growth substrate, while some others can degrade MTBE by co-metabolism, simultaneously consuming a different carbon source [19].…”

Section: Introductionmentioning

confidence: 99%

Biodegradation of Methyl Tertiary Butyl Ether (MTBE) by a Microbial Consortium in a Continuous Up-Flow Packed-Bed Biofilm Reactor: Kinetic Study, Metabolite Identification and Toxicity Bioassays

et al. 2016

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This study investigated the aerobic biodegradation of methyl tertiary-butyl ether (MTBE) by a microbial consortium in a continuous up-flow packed-bed biofilm reactor using tezontle stone particles as a supporting material for the biofilm. Although MTBE is toxic for microbial communities, the microbial consortium used here was able to resist MTBE loading rates up to 128.3 mg L-1 h-1, with removal efficiencies of MTBE and chemical oxygen demand (COD) higher than 90%. A linear relationship was observed between the MTBE loading rate and the MTBE removal rate, as well as between the COD loading rate and the COD removal rate, within the interval of MTBE loading rates from 11.98 to 183.71 mg L-1 h-1. The metabolic intermediate tertiary butyl alcohol (TBA) was not detected in the effluent during all reactor runs, and the intermediate 2-hydroxy butyric acid (2-HIBA) was only detected at MTBE loading rates higher than 128.3 mg L-1 h-1. The results of toxicity bioassays with organisms from two different trophic levels revealed that the toxicity of the influent was significantly reduced after treatment in the packed-bed reactor. The packed-bed reactor system used in this study was highly effective for the continuous biodegradation of MTBE and is therefore a promising alternative for detoxifying MTBE-laden wastewater and groundwater.

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Biodegradation of fuel oxygenates and their effect on the expression of a newly identified cytochrome P450 gene in Achromobacter xylosoxidans MCM2/2/1

Cited by 16 publications

References 30 publications

Enhanced bioremediation of oily sludge using co‐culture of specific bacterial and yeast strains

Enhanced bioremediation of oily sludge using co‐culture of specific bacterial and yeast strains

Methyl t-butyl ether-degrading bacteria for bioremediation and biocontrol purposes

Biodegradation of Methyl Tertiary Butyl Ether (MTBE) by a Microbial Consortium in a Continuous Up-Flow Packed-Bed Biofilm Reactor: Kinetic Study, Metabolite Identification and Toxicity Bioassays

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