Biodegradation of disperse textile dye Brown 3REL by newly isolatedBacillussp. VUS

Abstract

Aims: To isolate the potential micro‐organism for the degradation of textile disperse dye Brown 3 REL and to find out the reaction mechanism. Methods and Results: 16S rDNA analysis revealed an isolate from textile effluent contaminated soil as Bacillus sp. VUS and was able to degrade (100%) dye Brown 3REL within 8 h at static anoxic condition. A significant increase in the activities of lignin peroxidase, laccase and NADH‐DCIP reductase was observed up to complete decolourization of Brown 3REL. The optimum temperature required for degradation was 40°C and pH 6·5–12·0. Phyto‐toxicity and chemical oxygen demand revealed nontoxic products of dye degradation. The biodegradation was monitored by UV–VIS, FTIR spectroscopy and HPLC. The final products 6,8‐dichloro‐quinazoline‐4‐ol and cyclopentanone were characterized by gas chromatography‐mass spectrometry. This Bacillus sp. VUS also decolourized (80%) textile dye effluent within 12 h. Conclusions: This study suggests that Bacillus sp. VUS could be a useful tool for textile effluent treatment. Significance and Impact of the Study: The newly isolated Bacillus sp. VUS decolourized 16 textile dyes and textile dye effluent also. It achieved complete biodegradation of Brown 3REL. Phytotoxicity study demonstrated no toxicity of the biodegraded products for plants with respect to Triticum aestivum and Sorghum bicolor.

Dye Waste Treatment

“…On the other hand, 100% degradation of dye Brown 3REL by Bacillus sp. VUS was achieved within eight hours at static anoxic condition [17]. This bacterium was also able to degrade 80% of dye in textile effluent.…”

Bacterial diversity and composition in major fresh produce growing soils affected by physiochemical properties and geographic locations

“…Gan et al (2011) reported that different enzymes such as protocatechuate dioxygenases and aromatic ring hydroxylases are responsible for the cleavage of aromatic rings. Similarly, Dawkar et al (2008) reported that laccases are involved in the demethylation of aromatic compounds. Hydroxylation dioxygenases have been reported to be involved in oxidative deamination reactions forming phenol like compounds (Lin et al, 2010).…”

Microbial degradation of Textile Dye (Remazol Black B) by Bacillus spp. ETL-2012

“…Interest in the bioremediation of pollutants using bacteria has intensified in recent years, as many researches demonstrated the efficacy of bacterial bioremediation over fungal and actinomycetes. Many bacteria capable of reducing azo dyes reported were isolated from textile effluent contaminated sites [35]. The effluent sample collected from Ankleshwar textile industries, Ankleshwar, Gujarat, India, was black in color, with pungent smell and pH of slightly above neutral level and was within the permissible limits.…”