Biodegradation of Crude Oil by Individual Bacterial Strains and a Mixed Bacterial Consortium Isolated from Hydrocarbon Contaminated Areas

Sathishkumar, M.; Binupriya, Arthur Raj; Baik, Sang‐Ho; Yun, Soon‐Il

doi:10.1002/clen.200700042

Cited by 138 publications

(104 citation statements)

References 36 publications

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“…Thus, providing an optimum pH condition between pH 6-8.5 is supportive to bacterial population, and therefore, it is supporting the biodegradation process. Satishkumar et al (2008) found that pH ranging between 7 and 8 was more favorable for all the bacterial isolates and mixed bacterial consortium for degradation of crude oil. Similarly, Salmon et al (1998) reported that pH 7 was the optimum pH for degradation of hydrocarbon.…”

Section: Effect Of Npk and Bulking Agents On Enzyme Inductionmentioning

confidence: 95%

Induced degradation of crude oil mediated by microbial augmentation and bulking agents

Kumari

Singh

2016

Int. J. Environ. Sci. Technol.

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Different bacterial and fungal strains, isolated from petroleum hydrocarbon-contaminated soil, were tested, in isolation as well as in combination, for their ability to degrade total petroleum hydrocarbon (TPH) in soil samples spiked with crude oil (2, 5 or 10 %, w/w) for 30 days. The selected combination of bacterial and fungal isolates, i.e., Pseudomonas stutzeri BP10 and Aspergillus niger PS9, exhibited the highest efficiency of TPH degradation (46.7 %) in soil spiked with 2 % crude oil under control condition. Further, when this combination was applied under natural condition in soil spiked with 2 % (w/w) crude oil along with inorganic fertilizers (NPK) and different bulking agents such as rice husk, sugarcane, vermicompost or coconut coir, the percent degradation of TPH was found to be maximum (82.3 %) due to the presence of inorganic fertilizers and rice husk as bulking agent. Further, results showed that the presence of NPK and bulking agents induced the activity of degradative enzymes, such as catalase (0.718 m mol H 2 O 2 g -1 ), laccase (0.77 lmol g -1 ), dehydrogenase (37.5 lg g -1 h -1 ), catechol 1, 2 dioxygenase (276.11 l mol g -1 ) and catechol 2, 3 dioxygenase (15.15 l mol g -1 ) as compared to control (without bioaugmentation). It was inferred that the selected combination microbes along with biostimulants could accentuate the crude oil degradation as evident from the biostimulantinduced enhanced activity of degradative enzymes.

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Section: Effect Of Npk and Bulking Agents On Enzyme Inductionmentioning

confidence: 95%

Induced degradation of crude oil mediated by microbial augmentation and bulking agents

Kumari

Singh

2016

Int. J. Environ. Sci. Technol.

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“…The indigenous bacterial consortium consisting of three bacterial isolates was selected. The crude oil-degrading efficiency (qualitative and quantitative) of individual bacterial isolates Pseudomonas strains (PSI, PSII, PSIII) was screened on minimal salt medium as described elsewhere [9], using crude oil as the sole carbon and energy source [10]. These selected isolates were characterized and identified using biochemical tests and 16S rRNA sequencing from The Energy and Resources Institute (TERI) New Delhi, India.…”

Section: Source Of Bacterial Consortiummentioning

confidence: 99%

A Feasibility Study for Assessment of <i>In-situ</i> Bioremediation Potential of a Crude Oil Degrading <i>Pseudomonas</i> Consortium

Mittal

Singh

2009

J. Sci. Res.

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A microcosm study evaluating inoculums addition of mixed bacterial consortium to stimulate in-situ bioremediation of crude oil contaminated soil was conducted. In feasibility study, out of five treatments the application of bacterial consortium, nutrients and environmental factors resulted in 79.16% removal of TPH in 60 days, compared to 30.24% removal of TPH carried out by indigenous microflora. Gas chromatograms of original spilled oil and bioremediated oil by the addition of developed consortium shows that Pr/Ph ratio decreased progressively from 2.358 to 1.626. The results showed that the ratio of di/tri aromatics decreased from initial 0.63 to 0.25 with progressive treatment of nutrient addition, as nutrient + tilling, nutrient + tilling + microbial seeding. Similar effect was observed in di/di + tri aromatics ratios which also decreased 0.31 to 0.20 by bioaugmentation only.

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“…Microbial degradation can be accomplished by different species of microorganisms that are both native to the soil and added as effective degrading strains. The microbial degradation is generally higher than 40% within 1 year of disposal and may be as high as 70% in some cases (Sathishkumar et al, 2008). Influencing factors for microbial remediation include soil moisture content, soil temperature, soil pH, oxygen supply, nutrients, oxidation-reduction potential, soil texture and soil structure (Riser-Roberts, 1998).…”

Section: Introductionmentioning

confidence: 99%

Characterisation of the rhizoremediation of petroleum-contaminated soil: effect of different influencing factors

et al. 2010

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Abstract. Pilot experiments were conducted to analyse the effect of different environmental factors on the rhizoremediation of petroleum-contaminated soil. Different plant species (cotton, ryegrass, tall fescue and alfalfa), the addition of fertilizer, different concentrations of total petroleum hydrocarbons (TPH) in the soil, bioaugmentation with effective microbial agents (EMA) and plant growth-promoting rhizobacteria (PGPR) and remediation time were tested as influencing factors during the bioremediation process of TPH. The results show that the remediation process can be enhanced by different plant species. The order of effectiveness of the plants was the following: tall fescue > ryegrass > alfalfa > cotton. The degradation rate of TPH increased with increased fertilizer addition, and a moderate urea level of 20 g N (Nitrogen)/m 2 was best for both plant growth and TPH remediation. A high TPH content is toxic to plant growth and inhibits the degradation of petroleum hydrocarbons. The results showed that a 5% TPH content gave the best degradation in soil planted with ryegrass. Bioaugmentation with different bacteria and PGPR yielded the following results for TPH degradation: cotton+EMA+PGPR > cotton+EMA > cotton+PGPR > cotton > control. Rapid degradation of TPH was found at the initial period of remediation caused by the activity of microorganisms. A continuous increase of degradation rate was found during the 30-90 days period followed by a slow increase during the 90-150 days period. These results suggest that rhizoremediation can be enhanced with the proper control of different influencing factors that affect both plant growth and microbial activity in the rhizosphere environment.

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Biodegradation of Crude Oil by Individual Bacterial Strains and a Mixed Bacterial Consortium Isolated from Hydrocarbon Contaminated Areas

Cited by 138 publications

References 36 publications

Induced degradation of crude oil mediated by microbial augmentation and bulking agents

Induced degradation of crude oil mediated by microbial augmentation and bulking agents

A Feasibility Study for Assessment of <i>In-situ</i> Bioremediation Potential of a Crude Oil Degrading <i>Pseudomonas</i> Consortium

Characterisation of the rhizoremediation of petroleum-contaminated soil: effect of different influencing factors

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