Biodegradation of Chlorimuron-ethyl by the Bacterium<i>Klebsiella jilinsis</i>2N3

Zhang, Hao; Zhang, Xianghui; Mu, Wenhui; Wang, Jiaxiu; Pan, Hongyu; Liu, Yu

doi:10.1080/03601234.2010.493473

Cited by 25 publications

(9 citation statements)

References 23 publications

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“…Temperature is a dominant factor affecting the degradation of the recalcitrant compounds such as NHC 19 . The cell growth, metabolic and enzymatic activities can be significantly inhibited when the temperature is too high or too low.…”

Section: Resultsmentioning

confidence: 99%

Biodegradation mechanism of 1H-1,2,4-triazole by a newly isolated strain Shinella sp. NJUST26

Shen

et al. 2016

Sci Rep

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The highly recalcitrant 1H-1,2,4-triazole (TZ) is widely used in the synthesis of agricultural pesticide and considered to be an environmental pollutant. In this study, a novel strain NJUST26 capable of utilizing TZ as the sole carbon and nitrogen source, was isolated from TZ-contaminated soil, and identified as Shinella sp. The biodegradation assays suggested that optimal temperature and pH for TZ degradation by NJUST26 were 30 °C and 6–7, respectively. With the increase of initial TZ concentration from 100 to 320 mg L−1, the maximum volumetric degradation rate increased from 29.06 to 82.96 mg L−1 d−1, indicating high tolerance of NJUST26 towards TZ. TZ biodegradation could be accelerated through the addition of glucose, sucrose and yeast extract at relatively low dosage. The main metabolites, including 1,2-dihydro-3H-1,2,4-triazol-3-one (DHTO), semicarbazide and urea were identified. Based on these results, biodegradation pathway of TZ by NJUST26 was proposed, i.e., TZ was firstly oxidized to DHTO, and then the cleavage of DHTO ring occurred to generate N-hydrazonomethyl-formamide, which could be further degraded to biodegradable semicarbazide and urea.

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Section: Resultsmentioning

confidence: 99%

Biodegradation mechanism of 1H-1,2,4-triazole by a newly isolated strain Shinella sp. NJUST26

Shen

et al. 2016

Sci Rep

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“…The circle map of the 2N3 genome was drawn using Circos (Figure 1). The genome sequence of strain 2N3 was deposited in GenBank with an accession number CP025541 (Bioproject accession: PRJNA427082) under the name of K. pneumoniae , previously recognized as K. jilinsis [10].…”

Section: Resultsmentioning

confidence: 99%

“…The degradation of chlorimuron-ethyl by 2N3 is also comparable to the results obtained by other studies. For example, when cultured in a liquid medium at 30–35 °C with a pH value of 6–7, strain 2N3 was able to degrade 83.5% and 92.5% of the chlorimuron-ethyl with the initial concentrations of 20 mg/L and 100 mg/L, respectively [10]. Furthermore, the degradation of chlorimuron-ethyl by Stenotrophomonas maltophilia D310-3 [25], Hansschlegelia sp.…”

Section: Resultsmentioning

confidence: 99%

“…NIII2 is able to produce glycoprotein flocculant [6], and Klebsiella pneumoniae J1 displays specific adsorption of Sulfamethoxazole [7,8,9]. Furthermore, K. pneumoniae 2N3, a gram-negative bacterium in the family of Enterobacteriaceae, can degrade not only chlorimuron but also other sulfonylurea herbicides such as metsulfuron-methyl, tribenuron-methyl, rimsulfuron, ethametsulfuron, and nicosulfuron [10]. A recent study has proposed the pathway for the degradation of chlorimuron-ethyl by another species in the Enterobateriaceae family, Enterobacter ludwigii sp.…”

Section: Introductionmentioning

confidence: 99%

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Whole Genome Sequencing and Analysis of Chlorimuron-Ethyl Degrading Bacteria Klebsiella pneumoniae 2N3

Zhang

Hao

Zhang

et al. 2019

IJMS

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Klebsiella pneumoniae 2N3 is a strain of gram-negative bacteria that can degrade chlorimuron-ethyl and grow with chlorimuron-ethyl as the sole nitrogen source. The complete genome of Klebsiella pneumoniae 2N3 was sequenced using third generation high-throughput DNA sequencing technology. The genomic size of strain 2N3 was 5.32 Mb with a GC content of 57.33% and a total of 5156 coding genes and 112 non-coding RNAs predicted. Two hydrolases expressed by open reading frames (ORFs) 0934 and 0492 were predicted and experimentally confirmed by gene knockout to be involved in the degradation of chlorimuron-ethyl. Strains of ΔORF 0934, ΔORF 0492, and wild type (WT) reached their highest growth rates after 8–10 hours in incubation. The degradation rates of chlorimuron-ethyl by both ΔORF 0934 and ΔORF 0492 decreased in comparison to the WT during the first 8 hours in culture by 25.60% and 24.74%, respectively, while strains ΔORF 0934, ΔORF 0492, and the WT reached the highest degradation rates of chlorimuron-ethyl in 36 hours of 74.56%, 90.53%, and 95.06%, respectively. This study provides scientific evidence to support the application of Klebsiella pneumoniae 2N3 in bioremediation to control environmental pollution.

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“…Yang et al used a solution containing the chlorimuron-ethyl-degrading strain CHL1 to remediate chlorimuron-ethyl-contaminated soil and investigated the lifetime of CHL1 and changes in the abundance of fungi and bacteria over the bioremediation period [6]. Unfortunately, degrading bacteria do not live long in liquid media because such media are easily contaminated; additionally, the preservation of these bacteria in liquid media is unsatisfactory [7].…”

Section: Introductionmentioning

confidence: 99%

Bioremediation of Historically Chlorimuron-Ethyl-Contaminated Soil by Co-Culture Chlorimuron-Ethyl-Degrading Bacteria Combined with the Spent Mushroom Substrate

et al. 2020

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In this study, a novel chlorimuron-ethyl-degrading Pleurotus eryngiu-SMS-CB was successfully constructed for remediation of soil historically contaminated with chlorimuron-ethyl. The P. eryngiu-SMS-CB was prepared using efficient chlorimuron-ethyl-degrading cocultured bacteria, Rhodococcus sp. D310-1 and Enterobacter sp. D310-5, with spent mushroom substrate (SMS, a type of agricultural waste containing laccase) of Pleurotus eryngiu as a carrier. The chlorimuron-ethyl degradation efficiency in historically chlorimuron-ethyl-contaminated soil reached 93.1% at the end of 80 days of treatment with the P. eryngiu-SMS-CB. Although the P. eryngiu-SMS-CB altered the microbial community structure at the beginning of the 80 days, the bacterial population slowly recovered after 180 days; thus, the P. eryngiu-SMS-CB does not have an excessive effect on the long-term microbial community structure of the soil. Pot experiments indicated that contaminated soil remediation with P. eryngiu-SMS-CB reduced the toxic effects of chlorimuron-ethyl on wheat. This paper is the first to attempt to use chlorimuron-ethyl-degrading bacterial strains adhering to P. eryngiu-SMS to remediate historically chlorimuron-ethyl-contaminated soil, and the microbial community structure and P. eryngiu-SMS-CB activity in chlorimuron-ethyl-contaminated soil were traced in situ to evaluate the long-term effects of this remediation.

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Biodegradation of Chlorimuron-ethyl by the BacteriumKlebsiella jilinsis2N3

Cited by 25 publications

References 23 publications

Biodegradation mechanism of 1H-1,2,4-triazole by a newly isolated strain Shinella sp. NJUST26

Biodegradation mechanism of 1H-1,2,4-triazole by a newly isolated strain Shinella sp. NJUST26

Whole Genome Sequencing and Analysis of Chlorimuron-Ethyl Degrading Bacteria Klebsiella pneumoniae 2N3

Bioremediation of Historically Chlorimuron-Ethyl-Contaminated Soil by Co-Culture Chlorimuron-Ethyl-Degrading Bacteria Combined with the Spent Mushroom Substrate

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