Biodegradation of biodiesel-oil by <i>Cellulosimicrobium</i> sp. Isolated from Colombian Caribbean soils

Bertel-Sevilla, Angela; Cervantes-Ceballos, Leonor; Tirado-Ballestas, Irina; Maldonado-Rojas, Wilson; Alzate, Juan F.; Olívero-Verbel, Jesús

doi:10.1080/09593330.2018.1564798

Cited by 7 publications

(5 citation statements)

References 68 publications

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“…In addition, the use of Bacillus spp. was reported to be a biofuel producer and biodegradator of biodiesel and other pollutants (Lundberg et al 2013 ; Mukhtar et al 2019 ; Acevedo-Barrios et al 2019 ; Bertel-Sevilla et al 2020 ). Moreover, Mukhtar et al ( 2019 ) related the presence of halotolerant bacterial strains with lipases and hemicellulose degradation capacity, indicating the importance of these microorganisms in soil fertilization and plant metabolism.…”

Section: Resultsmentioning

confidence: 99%

“…The PCR reaction was performed with a hot start of 95 °C for 10 min, followed by 25 cycles of denaturation at 94 °C for 1 min, primer annealing at 43 °C for 1 min and primer extension at 72 °C for 1.5 min, followed by a final extension at 72 °C for 5.5 min. Amplified PCR products of the 16 S ribosomal gene were separated on 1.2% (w/v) agarose gels stained with ethidium bromide (10 mg/ml) and analyzed using a gel documentation system (IngGenius 3 System - Syngene) (Acevedo-Barrios et al 2019 ; Bertel-Sevilla et al 2020 ).…”

Section: Methodsmentioning

confidence: 99%

“…Bacteria from marine environments express genes for survival in saline conditions and have shown resilience in saturated matrices (Dalmaso et al 2015 ). They also produce unique metabolites, enhancing their potential for bioremediation efforts amidst toxic pollutants (Bertel-Sevilla et al 2020 ; Lee et al 2021 ).…”

Section: Introductionmentioning

confidence: 99%

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Bioprospecting of extremophilic perchlorate-reducing bacteria: report of promising Bacillus spp. isolated from sediments of the bay of Cartagena, Colombia

Acevedo-Barrios,

Tirado-Ballestas,

Bertel-Sevilla

et al. 2024

Biodegradation

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Three extremophile bacterial strains (BBCOL-009, BBCOL-014 and BBCOL-015), capable of degrading high concentrations of perchlorate at a range of pH (6.5 to 10.0), were isolated from Colombian Caribbean Coast sediments. Morphological features included Gram negative strain bacilli with sizes averaged of 1.75 × 0.95, 2.32 × 0.65 and 3.08 × 0.70 μm, respectively. The reported strains tolerate a wide range of pH (6.5 to 10.0); concentrations of NaCl (3.5 to 7.5% w/v) and KClO4− (250 to 10000 mg/L), reduction of KClO4− from 10 to 25%. LB broth with NaCl (3.5–30% w/v) and KClO4ˉ (250-10000 mg/L) were used in independent trials to evaluate susceptibility to salinity and perchlorate, respectively. Isolates increased their biomass at 7.5 % (w/v) NaCl with optimal development at 3.5 % NaCl. Subsequently, ClO4ˉ reduction was assessed using LB medium with 3.5% NaCl and 10000 mg/L ClO4ˉ. BBCOL-009, BBCOL-014 and BBCOL-015 achieved 10%, 17%, and 25% reduction of ClO4ˉ, respectively. The 16 S rRNA gene sequence grouped them as Bacillus flexus T6186-2, Bacillus marisflavi TF-11 (T), and Bacillus vietnamensis 15 − 1 (T) respectively, with < 97.5% homology. In addition, antimicrobial resistance to ertapenem, vancomycine, amoxicillin clavulanate, penicillin, and erythromycin was present in all the isolates, indicating their high adaptability to stressful environments. The isolated strains from marine sediments in Cartagena Bay, Colombia are suitable candidates to reduce perchlorate contamination in different environments. Although the primary focus of the study of perchlorate-reducing and resistant bacteria is in the ecological and agricultural realms, from an astrobiological perspective, perchlorate-resistant bacteria serve as models for astrobiological investigations.

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Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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Bioprospecting of extremophilic perchlorate-reducing bacteria: report of promising Bacillus spp. isolated from sediments of the bay of Cartagena, Colombia

Acevedo-Barrios,

Tirado-Ballestas,

Bertel-Sevilla

et al. 2024

Biodegradation

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“…Previous studies showed that the Cellulosimicrobium sp. isolated from soil, marine sediment, and sewage can degrade 2,4,5-trichlorophenoxyacetic acid and biodiesel-oil [ 28 , 29 , 30 ]; however, studies on inorganic sulfur conversion are scarce. Therefore, further exploration was conducted.…”

Section: Resultsmentioning

confidence: 99%

Extraction, Purification, Characterization and Application in Livestock Wastewater of S Sulfur Convertase

Zhai

Duan

et al. 2022

IJERPH

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Sulfide is a toxic pollutant in the farming environment. Microbial removal of sulfide always faces various biochemical challenges, and the application of enzymes for agricultural environmental remediation has promising prospects. In this study, a strain of Cellulosimicrobium sp. was isolated: numbered strain L1. Strain L1 can transform S2−, extracellular enzymes play a major role in this process. Next, the extracellular enzyme was purified, and the molecular weight of the purified sulfur convertase was about 70 kDa. The sulfur convertase is an oxidase with thermal and storage stability, and the inhibitor and organic solvent have little effect on its activity. In livestock wastewater, the sulfur convertase can completely remove S2−. In summary, this study developed a sulfur convertase and provides a basis for the application in environmental remediation.

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“…We isolated Gram-positive bacteria from soil, river water, river sediment, stream water, and rhizosphere sources in the Middle Atlantic region of the United States. It is important to emphasize that these bacteria are not specific to this geographical region and have been isolated from diverse environmental sources worldwide ( 46 – 51 ). We employed a modified contact-mediated competition assay ( 38 ) to explore the relative competitive fitness of B. pseudomallei with 14 environmental Gram-positive bacteria.…”

Section: Discussionmentioning

confidence: 99%

The Burkholderia pseudomallei hmqA-G Locus Mediates Competitive Fitness against Environmental Gram-Positive Bacteria

et al. 2021

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Burkholderia pseudomallei naturally resides in water, soil, and the rhizosphere and its success as an opportunistic pathogen is dependent on the ability to persist in these harsh habitats long enough to come into contact with a susceptible host. In addition to adapting to limiting nutrients and diverse chemical and physical challenges, B. pseudomallei also has to interact with a variety of microbial competitors.

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Biodegradation of biodiesel-oil by Cellulosimicrobium sp. Isolated from Colombian Caribbean soils

Cited by 7 publications

References 68 publications

Bioprospecting of extremophilic perchlorate-reducing bacteria: report of promising Bacillus spp. isolated from sediments of the bay of Cartagena, Colombia

Bioprospecting of extremophilic perchlorate-reducing bacteria: report of promising Bacillus spp. isolated from sediments of the bay of Cartagena, Colombia

Extraction, Purification, Characterization and Application in Livestock Wastewater of S Sulfur Convertase

The Burkholderia pseudomallei hmqA-G Locus Mediates Competitive Fitness against Environmental Gram-Positive Bacteria

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