The Burkholderia pseudomallei
            <i>hmqA-G</i>
            Locus Mediates Competitive Fitness against Environmental Gram-Positive Bacteria

Mou, Sherry; Jenkins, Conor; Okaro, Udoka; Dhummakupt, Elizabeth S.; Mach, Phillip M.; DeShazer, David

doi:10.1128/spectrum.00102-21

Cited by 7 publications

(8 citation statements)

References 62 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Microorganisms exist with complex interrelationships among the myriad of members of the community, and interspecific interactions are essential for community assembly and ecosystem functioning ( 25 – 30 ). Several mathematical methods (e.g., co-occurrence network analysis) have been developed to infer potential interactions among microorganisms in a community ( 31 ).…”

Section: Introductionmentioning

confidence: 99%

Litter Mixing Alters Microbial Decomposer Community to Accelerate Tomato Root Litter Decomposition

Xue

Wang

et al. 2022

Microbiol Spectr

View full text Add to dashboard Cite

show abstract

Section: Introductionmentioning

confidence: 99%

Litter Mixing Alters Microbial Decomposer Community to Accelerate Tomato Root Litter Decomposition

Xue

Wang

et al. 2022

Microbiol Spectr

View full text Add to dashboard Cite

show abstract

“…pseudomallei complex strains (including B. thailandensis), as well as in co-cultures comprised of Gram-positive bacteria. ,,,, The role of HMAQs in pathogenesis has been analyzed through phenotypic and sequencing-based studies of bacteria in monoculture as well as in co-cultures with other bacteria. ,, In our study, many of the HMAQs were detected in both B. thailandensis monocultures and B.…”

Section: Resultsmentioning

confidence: 64%

“…34 HMAQs were previously detected in monocultures of B. pseudomallei complex strains (including B. thailandensis), as well as in co-cultures comprised of Grampositive bacteria. 20,33,34,72,73 The role of HMAQs in pathogenesis has been analyzed through phenotypic and sequencingbased studies of bacteria in monoculture as well as in cocultures with other bacteria. 33,44,74 In our study, many of the HMAQs were detected in both B. thailandensis monocultures and B. thailandensis + LA-4 or RAW264.7 co-cultures, while some were detected exclusively in B. thailandensis monocultures (Figure S1).…”

Section: T H I S C O N T E N T Imentioning

confidence: 99%

Metabolomics Analysis of Bacterial Pathogen Burkholderia thailandensis and Mammalian Host Cells in Co-culture

et al. 2022

View full text Add to dashboard Cite

The Tier 1 HHS/USDA Select Agent Burkholderia pseudomallei is a bacterial pathogen that is highly virulent when introduced into the respiratory tract and intrinsically resistant to many antibiotics. Transcriptomic- and proteomic-based methodologies have been used to investigate mechanisms of virulence employed by B. pseudomallei and Burkholderia thailandensis, a convenient surrogate; however, analysis of the pathogen and host metabolomes during infection is lacking. Changes in the metabolites produced can be a result of altered gene expression and/or post-transcriptional processes. Thus, metabolomics complements transcriptomics and proteomics by providing a chemical readout of a biological phenotype, which serves as a snapshot of an organism’s physiological state. However, the poor signal from bacterial metabolites in the context of infection poses a challenge in their detection and robust annotation. In this study, we coupled mammalian cell culture-based metabolomics with feature-based molecular networking of mono- and co-cultures to annotate the pathogen’s secondary metabolome during infection of mammalian cells. These methods enabled us to identify several key secondary metabolites produced by B. thailandensis during infection of airway epithelial and macrophage cell lines. Additionally, the use of in silico approaches provided insights into shifts in host biochemical pathways relevant to defense against infection. Using chemical class enrichment analysis, for example, we identified changes in a number of host-derived compounds including immune lipids such as prostaglandins, which were detected exclusively upon pathogen challenge. Taken together, our findings indicate that co-culture of B. thailandensis with mammalian cells alters the metabolome of both pathogen and host and provides a new dimension of information for in-depth analysis of the host–pathogen interactions underlying Burkholderia infection.

show abstract

“…A lack of travel history for the two Texas cases and phylogeographic analysis of the infecting strains strongly suggest these two infections were independently acquired from the local environment in Texas. Detecting B. pseudomallei in the environment has been historically challenging due to the incomplete understanding of its optimal habitat (e.g., soil composition, moisture content, presence of competing bacteria [29][30][31]). However, our findings are consistent with the idea that B. pseudomallei is quite rare in the environment in this region of Texas.…”

Section: Discussionmentioning

confidence: 99%

Low risk of acquiring melioidosis from the environment in the continental United States

et al. 2022

View full text Add to dashboard Cite

Melioidosis is an underreported human disease of tropical and sub-tropical regions caused by the saprophyte Burkholderia pseudomallei. Although most global melioidosis cases are reported from tropical regions in Southeast Asia and northern Australia, there are multiple occurrences from sub-tropical regions, including the United States (U.S.). Most melioidosis cases reported from the continental U.S. are the result of acquiring the disease during travel to endemic regions or from contaminated imported materials. Only two human melioidosis cases from the continental U.S. have likely acquired B. pseudomallei directly from local environments and these cases lived only ~7 km from each other in rural Texas. In this study, we assessed the risk of acquiring melioidosis from the environment within the continental U.S. by surveying for B. pseudomallei in the environment in Texas where these two human melioidosis cases likely acquired their infections. We sampled the environment near the homes of the two cases and at additional sampling locations in surrounding counties in Texas that were selected based on ecological niche modeling. B. pseudomallei was not detected at the residences of these two cases or in the surrounding region. These negative data are important to demonstrate that B. pseudomallei is rare in the environment in the U.S. even at locations where locally acquired human cases likely have occurred, documenting the low risk of acquiring B. pseudomallei infection from the environment in the continental U.S.

show abstract

The Burkholderia pseudomallei hmqA-G Locus Mediates Competitive Fitness against Environmental Gram-Positive Bacteria

Cited by 7 publications

References 62 publications

Litter Mixing Alters Microbial Decomposer Community to Accelerate Tomato Root Litter Decomposition

Litter Mixing Alters Microbial Decomposer Community to Accelerate Tomato Root Litter Decomposition

Metabolomics Analysis of Bacterial Pathogen Burkholderia thailandensis and Mammalian Host Cells in Co-culture

Low risk of acquiring melioidosis from the environment in the continental United States

Contact Info

Product

Resources

About