Biodegradation of aflatoxin by bacterial species isolated from poultry farms

Ali, Sabah; Hassan, Mariam; Essam, Tamer; Ibrahim, Marwa A.; Al-Amry, Khaled

doi:10.1016/j.toxicon.2021.02.005

Cited by 21 publications

(19 citation statements)

References 49 publications

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“…Firstly, B. amyloliquefaciens WF2020 can degrade AFB1 ranging from 1 to 5 μg/ml by more than 80% after a 72-h incubation, which was similar to the 85.50% reduction of AFB1 at the concentration of 0.5 μg/ml reported in B. amyloliquefaciens SG16 ( Wang J. et al, 2018 ) and was significantly higher than the 42.13 and 58.77% reduction reported in B. amyloliquefaciens SWUN-TP23 and HSP-5, respectively ( Xu et al, 2015 ; Guo et al, 2017 ) and the 40 and 73.2% reduction of AFB1 at the concentration of 0.5 μg/ml reported in B. amyloliquefaciens S8C and Y1-B1, respectively ( Ali et al, 2021 ; Zhang et al, 2021 ). Compared with the degradation abilities of AFB1 in the reported Bacillus species, the degradation ability in B. amyloliquefaciens WF2020 is similar to that in B. licheniformis BL010 ( Wang Y. et al, 2018 ), B. velezensis DY3108 ( Shu et al, 2018 ), and B. subtilis UTBSP1 ( Farzaneh et al, 2012 ), higher than that in B. subtilis JSW-1 ( Xia et al, 2017 ) but slightly lower than that in Bacillus sp.…”

Section: Discussionmentioning

confidence: 88%

“…Generally, B. amyloliquefaciens was considered as a safe and non-toxic producing microbe and could be used for food and pharmaceutical purposes ( WoldemariamYohannes et al, 2020 ). It was also reported that some strains of B. amyloliquefaciens , such as B. amyloliquefaciens UTB2, UNRC52, UNRCLR, S8C, Y1-B1, SWUN-TP23, SG-16, and HSP-5, could inhibit AFB1 synthesis or accelerate AFB1 degradation ( Bluma and Etcheverry, 2006 ; Xu et al, 2015 ; Guo et al, 2017 ; Siahmoshteh et al, 2018 ; Wang J. et al, 2018 ; Ali et al, 2021 ; Zhang et al, 2021 ). Here, our results indicated that B. amyloliquefaciens WF2020 derived from naturally fermented pickles could act as a potential probiotic to efficiently detoxify AFB1 in a time dependent manner in ranges of 1–8 μg/ml and inhibit the fungal growth of A. flavus and AFB1 production, as discussed below.…”

Section: Discussionmentioning

confidence: 99%

“…In addition, some strains of B. amyloliquefaciens , such as B. amyloliquefaciens UTB2, UNRC52, and UNRCLR, could suppress AFB1 synthesis ( Bluma and Etcheverry, 2006 ; Siahmoshteh et al, 2018 ). However, except for B. amyloliquefaciens S8C, Y1-B1, SWUN-TP23, SG-16, and HSP-5 ( Xu et al, 2015 ; Guo et al, 2017 ; Wang J. et al, 2018 ; Ali et al, 2021 ; Zhang et al, 2021 ), little was known about the AFB1 degradation potential of B. amyloliquefaciens as well as the molecular mechanism of the loss in AFB1 production. Here, the AFB1-degrading bacterium in naturally fermented pickles was isolated and identified as B. amyloliquefaciens (WF2020), and the toxicities of the strain and its AFB1 degradation products were also assessed based on sequenced genome information, antibiotic susceptibility, the changes in the lifespan of Caenorhabditis elegans , and Ames mutagenicity.…”

Section: Introductionmentioning

confidence: 99%

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Detoxification of Aflatoxin B1 by a Potential Probiotic Bacillus amyloliquefaciens WF2020

et al. 2022

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Microbial degradation is considered as an attractive method to eliminate exposure to aflatoxin B1 (AFB1), the most toxic mycotoxin that causes great economic losses and brings a serious threat to human and animal health, in food and feed. In this study, Bacillus amyloliquefaciens WF2020, isolated from naturally fermented pickles, could effectively degrade AFB1 ranging from 1 to 8 μg/ml, and the optimum temperature and pH value were 37–45°C and 8.0, respectively. Moreover, B. amyloliquefaciens WF2020 was considered to be a potential probiotic due to the synthesis of active compounds, absence of virulence genes, susceptibility to various antibiotics, and enhanced lifespan of Caenorhabditis elegans. Extracellular enzymes or proteins played a major role in AFB1 degradation mediated by B. amyloliquefaciens WF2020 into metabolites with low or no mutagenicity and toxicity to C. elegans. AFB1 degradation by the cell-free supernatant was stable up to 70°C, with an optimal pH of 8.0, and the cell-free supernatant could still degrade AFB1 by 37.16% after boiling for 20 min. Furthermore, B. amyloliquefaciens WF2020 caused a slight defect in fungal growth and completely inhibited AFB1 production when co-incubated with Aspergillus flavus. Additionally, B. amyloliquefaciens WF2020 suppressed the expression of 10 aflatoxin pathway genes and 2 transcription factors (alfR and alfS), suggesting that B. amyloliquefaciens WF2020 might inhibit AFB1 synthesis in A. flavus. These results indicate that B. amyloliquefaciens WF2020 and/or its extracellular enzymes or proteins have a promising potential to be applied in protecting food and feed from AFB1 contamination.

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Section: Discussionmentioning

confidence: 88%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Detoxification of Aflatoxin B1 by a Potential Probiotic Bacillus amyloliquefaciens WF2020

et al. 2022

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“…It should be noted that the direct use of food waste in poultry feed without their processing can lead to the spread of pathogens and infections. Therefore, proper processing and disinfection of these materials is an important issue that requires great care (Ali et al, 2021;Arifjanov et al, 2021). Wet and unprocessed feed, due to its high moisture content, disrupts nutrition, reducing dry matter intake by reducing product shelf life.…”

Section: Introductionmentioning

confidence: 99%

Investigation of parameters in restaurant food waste for use as poultry rations

et al. 2022

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One way to use food industry wastes is to feed the livestock and poultry. Applying appropriate management methods for the optimal use of agricultural waste in the preparation of livestock and poultry feed increases productivity and reduces damage to agricultural and conversion industries and the use of new resources in animal and poultry feed. In this paper, in order to investigate the possibility of recycling food waste as poultry feed, the waste produced in a restaurant has been used. The parameters of fat percentage were measured using Soxhlet extractor, percentage of dry matter using the oven, percentage of protein in it using Kjeldahl method, and also the percentage of calcium and phosphorus with the standard beard. Comparing them with the usual diet used in poultry feed shows that the average values of calcium, protein, and fat content of food waste are 0.14%, 5.65%, 7.34% higher than the average diet of poultry, respectively. Also, the average amount of phosphorus and dry matter of food waste is 0.48% and 60% less than the average diet of poultry, respectively.

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“…Minimal mineral salt medium (MMSM) was prepared according to method described by Ali and coworkers (Ali et al 2021 ). The medium was composed of 10 g FeSO 4 .7H 2 O, 4 g K2HPO4, 4 g KH2PO4, 10 g CaCl 2 .2H 2 O, 100 g MgSO 4 .7H 2 O, 3 g (NH 4 ) 2 SO 4 , and trace elements 1 mL −1 .…”

Section: Methodsmentioning

confidence: 99%

Acinetobacter baumannii, Klebsiella pneumoniae and Elizabethkingia miricola isolated from wastewater have biodegradable activity against fluoroquinolone

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Nagy

Adly

et al. 2022

World J Microbiol Biotechnol

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Ciprofloxacin (CIP) and levofloxacin (LEV), widely used fluoroquinolone antibiotics, are often found in sewage from the sewage treatment plants and marine environment. In this study, CIP and LEV biodegrading bacterial consortia were obtained from industrial wastewater. Microorganisms in these consortia were identified as Acinetobacter baumannii (A. baumannii), Klebsiella pneumoniae (K. pneumoniae) and Elizabethkingia miricola (E. miricola). The impacts of the critical operating parameters on the elimination of CIP and LEV by bacterial consortia have been investigated and optimized to achieve the maximum levels of CIP and LEV biodegradation. Using liquid chromatography with tandem mass spectrometry (LC-MS-MS), possible degradation pathways for CIP and LEV were suggested by analyzing the intermediate degradation products. The role of the enzymes fluoroquinolone-acetylating aminoglycoside (6′-N-acetyltransferase) and cytochrome P450 (CYP450) in the breakdown of fluoroquinolones (FQs) was investigated as well. According to our findings, various biodegradation mechanisms have been suggested, including cleavage of piperazine ring, substitution of F atom, hydroxylation, decarboxylation, and acetylation, as the main biotransformation reactions. This study discovers the ability of non-reported bacterial strains to biodegrade both CIP and LEV as a sole carbon source, providing new insights into the biodegradation of CIP and LEV. Graphical abstract

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Biodegradation of aflatoxin by bacterial species isolated from poultry farms

Cited by 21 publications

References 49 publications

Detoxification of Aflatoxin B1 by a Potential Probiotic Bacillus amyloliquefaciens WF2020

Detoxification of Aflatoxin B1 by a Potential Probiotic Bacillus amyloliquefaciens WF2020

Investigation of parameters in restaurant food waste for use as poultry rations

Acinetobacter baumannii, Klebsiella pneumoniae and Elizabethkingia miricola isolated from wastewater have biodegradable activity against fluoroquinolone

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