Biodegradable β-tricalcium phosphate cement with anti-washout property based on chelate-setting mechanism of inositol phosphate

Abstract

Novel biodegradable β-tricalcium phosphate (β-TCP) cements with anti-washout properties were created on the basis of chelate-setting mechanism of inositol phosphate (IP6) using β-TCP powders. The β-TCP powders were ball-milled using ZrO₂ beads for 0-6 h in the IP6 solutions with concentrations from 0 to 10,000 ppm. The chelate-setting β-TCP cement with anti-washout property was successfully fabricated by mixing the β-TCP powder ball-milled in 3,000 ppm IP6 solution for 3 h and 2.5 mass% Na₂HPO₄ solution, and compressive strength of the cement was 13.4 ± 0.8 MPa. An in vivo study revealed that the above cement was directly in contact with host and newly formed bones without fibrous tissue layers, and was resorbed by osteoclast-like cells on the surface of the cement. The chelate-setting β-TCP cement with anti-washout property is promising for application as a novel injectable artificial bone with both biodegradability and osteoconductivity.

Fabrication of chelate‐setting α‐tricalcium phosphate cement using sodium citrate and sodium alginate as mixing solution and its in vivo osteoconductivity

“…After implanting for 4 weeks, the pig was sacrificed and the tibia was removed and embedded using a super‐cryoembedding medium (Leica Microsystems, Japan). The resulting frozen blocks were sectioned at 16 μm using a rotary microtome (CM3050S, Leica Microsystems), according to our previous report . The thin sections stained with toluidine blue were observed with an upright optical microscope (BX41, Olympus, Japan).…”

“…The resulting frozen blocks were sectioned at 16 lm using a rotary microtome (CM3050S, Leica Microsystems), according to our previous report. 19,20 The thin sections stained with toluidine blue were observed with an upright optical microscope (BX41, Olympus, Japan). Moreover, the cement was isolated from one of the embedded blocks, and the crystal phase of the cement was determined using the XRD.…”

Evaluation of resistance to fragmentation of injectable calcium-phosphate cement paste using X-ray microcomputed tomography

“…14) In brief, commercially-available ¢-TCP (¢-TCP-100, Taihei Chemical Industrial Co. Ltd., Japan) powder (10 g) was placed into a 3,000 ppm 40-cm 3 IP6 (50% phytic acid, Wako Pure Chemical Industries. Co., Japan) solution or pure water in a zirconia pot.…”

Antibacterial polyetheretherketone implants immobilized with silver ions based on chelate-bonding ability of inositol phosphate: Processing, material characterization, cytotoxicity, and antibacterial properties

“…One of the present authors and his coworkers successfully developed a novel calcium phosphate cement (CPC) using inositol hexaphosphate [C 6 H 6 (OPO 3 H 2 ) 6 ; IP6] as a chelating agent . IP6 is present in grains of wheat, rice, and soybean; thus, it has a strong chelating capacity for Ca 2+ ions, similarly to ethylenediaminetetraacetic acid (EDTA).…”

“…19 One of the present authors and his coworkers successfully developed a novel calcium phosphate cement (CPC) using inositol hexaphosphate [C 6 H 6 (OPO 3 H 2 ) 6 ; IP6] as a chelating agent. [20][21][22][23][24] IP6 is present in grains of wheat, rice, and soybean 25 ; thus, it has a strong chelating capacity for Ca 21 ions, similarly to ethylenediaminetetraacetic acid (EDTA). The paste containing HAp particles surface-modified with IP6 and pure water set to form a cement specimen based on chelate-bonding of IP6 among individual Ca 21 ions in HAp particles.…”