Biodegradable stent coatings on the basis of PLGA polymers of different molecular mass, sustaining a steady release of the thrombolityc enzyme streptokinase

Sevostyanov, M. A.; Баикин, А. С.; Sergienko, K. V.; Shatova, L. A.; Kirsankin, A. A.; Baymler, Ilya V.; Shkirin, A. V.; Gudkov, Sergey V.

doi:10.1016/j.reactfunctpolym.2020.104550

Cited by 26 publications

(13 citation statements)

References 47 publications

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“…Streptokinase-loaded PLGA membranes were successfully evaluated as coatings for nitinol stents, as they enabled a burst release of the antithrombotic agent after 3 days and its sustained slow release for 30 days. For a period of 60 days post-implantation, no allergic and immune reactions nor cardiac events were noticed [ 83 ].…”

Section: Resultsmentioning

confidence: 99%

Bioactive Ibuprofen-Loaded PLGA Coatings for Multifunctional Surface Modification of Medical Devices

et al. 2021

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To modulate the biofunctionality of implantable medical devices commonly used in clinical practice, their surface modification with bioactive polymeric coatings is an attractive and successful emerging strategy. Biodegradable coatings based on poly(lactic acid-co-glycolic acid), PLGA, represent versatile and safe candidates for surface modification of implantable biomaterials and devices, providing additional tunable ability for topical delivery of desired therapeutic agents. In the present study, Ibuprofen-loaded PLGA coatings (PLGA/IBUP) were obtained by using the dip-coating and drop-casting combined protocol. The composite materials demonstrated long-term drug release under biologically simulated dynamic conditions. Reversible swelling phenomena of polymeric coatings occurred in the first two weeks of testing, accompanied by the gradual matrix degradation and slow release of the therapeutic agent. Irreversible degradation of PLGA coatings occurred after one month, due to copolymer’s hydrolysis (evidenced by chemical and structural modifications). After 30 days of dynamic testing, the cumulative release of IBUP was ~250 µg/mL. Excellent cytocompatibility was revealed on human-derived macrophages, fibroblasts and keratinocytes. The results herein evidence the promising potential of PLGA/IBUP coatings to be used for surface modification of medical devices, such as metallic implants and wound dressings.

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Section: Resultsmentioning

confidence: 99%

Bioactive Ibuprofen-Loaded PLGA Coatings for Multifunctional Surface Modification of Medical Devices

et al. 2021

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“…Pharmaceutics, regardless of their molar weight, can be loaded into PLGA drug delivery systems and consequently released from the matrix in a controlled manner. Consequently, a wide variety of active compounds can be used as payload of PLGA based drug delivery systems ranging from small molecules such as anti-tumour drugs with molar weight 0.5 kDa [ 15 ] to proteins and enzymes with molar mass up to 50 kDa [ 16 , 17 ]. The drug release profile of PLGA based drug delivery systems is usually characterized by the initial burst of a loaded drug followed by zero order kinetics release [ 18 ].…”

Section: Introductionmentioning

confidence: 99%

PLGA Based Nanospheres as a Potent Macrophage-Specific Drug Delivery System

et al. 2021

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Macrophages possess an innate ability to scavenge heterogenous objects from the systemic circulation and to regulate inflammatory diseases in various organs via cytokine production. That makes them attractive targets for nanomedicine-based therapeutic approaches to inflammatory diseases. In the present study, we have prepared several different poly(lactic-co-glycolic acid) (PLGA) polymer nanospheres for macrophage-targeted drug delivery using both nanoprecipitation and emulsification solvent evaporation methods. Two experimental linear PLGA polymers with relatively low molar weight, one experimental branched PLGA with unique star-like molecular architecture, and a commercially available PLGA, were used for nanosphere formulation and compared to their macrophage uptake capacity. The nanosphere formulations labelled with loaded fluorescent dye Rhodamine B were further tested in mouse bone marrow-derived macrophages and in hepatocyte cell lines AML-12, HepG2. We found that nanospheres larger than 100 nm prepared using nanoprecipitation significantly enhanced distribution of fluorescent dye selectively into macrophages. No effects of nanospheres on cellular viability were observed. Additionally, no significant proinflammatory effect after macrophage exposure to nanospheres was detected as assessed by a determination of proinflammatory cytokines Il-1β and Tnfα mRNA. All experimental PLGA nanoformulations surpassed the nanospheres obtained with the commercially available polymer taken as a control in their capacity as macrophage-specific carriers.

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“…Streptokinase was used to dissolve trapped microscopic clots in the trophoblastic and spongy layer of the amniochorionic membrane. Streptokinase is a thrombolytic enzyme that is used in the treatment of patients with pulmonary thromboembolism and myocardial infarctions 46 . Additionally, this fibrinolytic enzyme can also unbind cellular adhesions and help to decellularize the natural substrate 47 .…”

Section: Discussionmentioning

confidence: 99%

Developing a pro-angiogenic placenta derived amniochorionic scaffold with two exposed basement membranes as substrates for cultivating endothelial cells

Shariatzadeh

Shafiee

Zafari

et al. 2021

Sci Rep

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Decellularized and de-epithelialized placenta membranes have widely been used as scaffolds and grafts in tissue engineering and regenerative medicine. Exceptional pro-angiogenic and biomechanical properties and low immunogenicity have made the amniochorionic membrane a unique substrate which provides an enriched niche for cellular growth. Herein, an optimized combination of enzymatic solutions (based on streptokinase) with mechanical scrapping is used to remove the amniotic epithelium and chorion trophoblastic layer, which resulted in exposing the basement membranes of both sides without their separation and subsequent damages to the in-between spongy layer. Biomechanical and biodegradability properties, endothelial proliferation capacity, and in vivo pro-angiogenic capabilities of the substrate were also evaluated. Histological staining, immunohistochemistry (IHC) staining for collagen IV, and scanning electron microscope demonstrated that the underlying amniotic and chorionic basement membranes remained intact while the epithelial and trophoblastic layers were entirely removed without considerable damage to basement membranes. The biomechanical evaluation showed that the scaffold is suturable. Proliferation assay, real-time polymerase chain reaction for endothelial adhesion molecules, and IHC demonstrated that both side basement membranes could support the growth of endothelial cells without altering endothelial characteristics. The dorsal skinfold chamber animal model indicated that both side basement membranes could promote angiogenesis. This bi-sided substrate with two exposed surfaces for cultivating various cells would have potential applications in the skin, cardiac, vascularized composite allografts, and microvascular tissue engineering.

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Biodegradable stent coatings on the basis of PLGA polymers of different molecular mass, sustaining a steady release of the thrombolityc enzyme streptokinase

Cited by 26 publications

References 47 publications

Bioactive Ibuprofen-Loaded PLGA Coatings for Multifunctional Surface Modification of Medical Devices

Bioactive Ibuprofen-Loaded PLGA Coatings for Multifunctional Surface Modification of Medical Devices

PLGA Based Nanospheres as a Potent Macrophage-Specific Drug Delivery System

Developing a pro-angiogenic placenta derived amniochorionic scaffold with two exposed basement membranes as substrates for cultivating endothelial cells

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