Biodegradable self-assembled nanoparticles of poly (d,l-lactide-co-glycolide)/hyaluronic acid block copolymers for target delivery of docetaxel to breast cancer

Huang, Jingbin; Zhang, He; Yuan, Yu; Cao, Yan; Wang, Dong; Zhang, Guoqing; Zhou, Guichen; Liu, Junjie; Sun, Zhiguo; Sun, Duxin; Lü, Ying; Zhong, Yanqiang

doi:10.1016/j.biomaterials.2013.09.089

Cited by 191 publications

(107 citation statements)

References 39 publications

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“…Moreover, such low CMC value of HA-VES is conductive for the structural stability of micelles, which avoids the rapid release of encapsulated drugs from micelles in the systemic circulation before they reached the tumor sites. 37 The potential hemolytic activity of HA-VES micelles is one of the most important issues and needs to be determined to evaluate the biocompatibility of copolymers before they are injected into the vein. Hemolysis of HA-VES with different DS and Tween 80 was compared with that of 2% Triton X-100, a surfactant that is considered to have 100% hemolysis (shown in Supplementary materials).…”

Section: Results and Discussion Synthesis And Characterization Of Ha-mentioning

confidence: 99%

The effect of dual-functional hyaluronic acid-vitamin E succinate micelles on targeting delivery of doxorubicin

Wang¹,

Guo

et al. 2016

IJN

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Section: Results and Discussion Synthesis And Characterization Of Ha-mentioning

confidence: 99%

The effect of dual-functional hyaluronic acid-vitamin E succinate micelles on targeting delivery of doxorubicin

Wang¹,

Guo

et al. 2016

IJN

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“…While in the animals treated with Taxotere ® , body weight showed a significant decrease until the treatment was continued (Figure 6), which indicates the toxicity of the formulation, especially because it was accompanied by a notable loss of activity in animals. Among the animals that were treated with targeted and non-targeted DTX-NPs, the body weight remained almost constant (Figure 6), which could be interpreted as an advantage of the proposed DTX delivery system in comparison to those which cause body weight loss in the animals such as the PLGA/hyaluronic acid NPs reported by Huang et al 41 …”

Section: Discussionmentioning

confidence: 75%

Pharmacokinetics and in vitro/in vivo antitumor efficacy of aptamer-targeted Ecoflex<sup>®</sup> nanoparticles for docetaxel delivery in ovarian cancer

Ghassami¹,

Varshosaz²,

Jahanian-Najafabadi³

et al. 2018

IJN

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Purpose Epithelixal ovarian cancer is the fourth cause of cancer death in developed countries with 77% of ovarian cancer cases diagnosed with regional or distant metastasis, with poor survival rates. Docetaxel (DTX) is a well-known anticancer agent, with clinically proven efficacy in several malignancies, including ovarian cancer. However, the adverse effects caused by the active ingredient or currently marketed formulations could even deprive the patient of the advantages of treatment. Therefore, in the current study, polymeric nanoparticles (NPs) equipped with aptamer molecules as targeting agents were proposed to minimize the adverse effects and enhance the antitumor efficacy through directing the drug cargo toward its site of action. Materials and methods Electrospraying technique was implemented to fabricate poly (butylene adipate-co-butylene terephthalate) (Ecoflex ® ) NPs loaded with DTX (DTX-NPs). Afterward, aptamer molecules were added to the DTX-NPs, which bound via covalent bonds (Apt-DTX-NPs). The particle size, size distribution, zeta potential, entrapment efficiency, and release profile of the NPs were characterized. Using MTT assay and flow-cytometry analysis, the in vitro cytotoxicity and cellular uptake of the NPs were compared to those of the free drug. Following intravenous administration of Taxotere ® , DTX-NPs, and Apt-DTX-NPs (at an equivalent dose of 5 mg/kg of DTX), pharmacokinetic parameters and antitumor efficacy were compared in female Balb/c and HER-2-overexpressing tumor-bearing B6 athymic mice, respectively. Results The obtained results demonstrated significantly enhanced in vitro cytotoxicity and cellular uptake of Apt-DTX-NPs in a HER-2-overexpressing cell line, comparing to DTX-NPs and the free drug. The results of in vivo studies indicated significant increment in pharmacokinetic parameters including the area under the plasma concentration–time curve, mean residence time, and elimination half-life. Significant increment in antitumor efficacy was also observed, probably due to the targeted delivery of DTX to the tumor site and enhanced cellular uptake as evaluated in the aforementioned tests. Conclusion Hence, the proposed drug delivery system could be considered as an appropriate potential substitute for currently marketed DTX formulations.

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“…According to previous literature reports, 39,40 MDA-MB-231 and MCF-7 cells express 2×10 5 and 1×10 4 EGFR per cell and exhibit positive and negative expression of CD44, respectively. MDA-MB-231 cells were cultured in high-glucose Dulbecco's Modified Eagle's Medium (DMEM) with 10% fetal bovine serum and 1% penicillin-streptomycin at 37°C in 5% CO 2 atmosphere.…”

Section: Cell Culture and Animalsmentioning

confidence: 73%

“…It has been demonstrated that the CD44 and EGFR are highly overexpressed in MDA-MB-231 cells compared with those in MCF-7 cells. 39,40 We further proved that free HA and GE11 peptide suppressed the uptake of GE11-HA-ss-Chol 7 C6-NPs, and the specificity of uptake was verified by competitive inhibitory studies. Therefore, it could be concluded that the cellular uptake of GE11-HA-ss-Chol 7 C6-NPs involves the interaction with CD44 and EGFR in both cells, suggesting the existence of a dual-ligand tumor-targeting mechanism.…”

mentioning

confidence: 64%

GE11 peptide modified and reduction-responsive hyaluronic acid-based nanoparticles induced higher efficacy of doxorubicin for breast carcinoma therapy

Hu¹,

Mezghrani²,

Zhang³

et al. 2016

IJN

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Novel breast carcinoma dual-targeted redox-responsive nanoparticles (NPs) based on cholesteryl-hyaluronic acid conjugates were designed for intracellular delivery of the antitumor drug doxorubicin (DOX). A series of reduction-responsive hyaluronic acid derivatives grafted with hydrophobic cholesteryl moiety (HA-ss-Chol) and GE11 peptide conjugated HA-ss-Chol (GE11–HA-ss-Chol) were synthesized. The obtained conjugates showed attractive self-assembly characteristics and high drug loading capacity. GE11–HA-ss-Chol NPs were highly stable under conditions mimicking normal physiological conditions, while showing a fast degradation of the vehicle’s structure and accelerating the drug release dramatically in the presence of intracellular reductive environment. Furthermore, the cellular uptake assay confirmed GE11–HA-ss-Chol NPs were taken up by MDA-MB-231 cells through CD44- and epidermal growth factor receptor-mediated endocytosis. The internalization pathways of GE11–HA-ss-Chol NPs might involve clathrin-mediated endocytosis and macropinocytosis. The intracellular distribution of DOX in GE11–HA-ss-Chol NPs showed a faster release and more efficient nuclear delivery than the insensitive control. Enhanced in vitro cytotoxicity of GE11–HA-ss-Chol DOX-NPs further confirmed the superiority of their dual-targeting and redox-responsive capacity. Moreover, in vivo imaging investigation in MDA-MB-231 tumor-bearing mice confirmed that GE11–HA-ss-Chol NPs labeled with 1,1′-dioctadecyl-3,3,3′,3′-tetramethylindotricarbocyanine iodide, a near-infrared fluorescence dye, possessed a preferable tumor accumulation ability as compared to the single-targeting counterpart (HA-ss-Chol NPs). The antitumor efficacy showed an improved therapy efficacy and lower systemic side effect. These results suggest GE11–HA-ss-Chol NPs provide a good potential platform for antitumor drugs.

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Biodegradable self-assembled nanoparticles of poly (d,l-lactide-co-glycolide)/hyaluronic acid block copolymers for target delivery of docetaxel to breast cancer

Cited by 191 publications

References 39 publications

The effect of dual-functional hyaluronic acid-vitamin E succinate micelles on targeting delivery of doxorubicin

The effect of dual-functional hyaluronic acid-vitamin E succinate micelles on targeting delivery of doxorubicin

Pharmacokinetics and in vitro/in vivo antitumor efficacy of aptamer-targeted Ecoflex<sup>®</sup> nanoparticles for docetaxel delivery in ovarian cancer

GE11 peptide modified and reduction-responsive hyaluronic acid-based nanoparticles induced higher efficacy of doxorubicin for breast carcinoma therapy

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Biodegradable self-assembled nanoparticles of poly (d,l-lactide-co-glycolide)/hyaluronic acid block copolymers for target delivery of docetaxel to breast cancer

Cited by 191 publications

References 39 publications

The effect of dual-functional hyaluronic acid-vitamin E succinate micelles on targeting delivery of doxorubicin

The effect of dual-functional hyaluronic acid-vitamin E succinate micelles on targeting delivery of doxorubicin

Pharmacokinetics and in vitro/in vivo antitumor efficacy of aptamer-targeted Ecoflex<sup>&reg;</sup> nanoparticles for docetaxel delivery in ovarian cancer

GE11 peptide modified and reduction-responsive hyaluronic acid-based nanoparticles induced higher efficacy of doxorubicin for breast carcinoma therapy

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Pharmacokinetics and in vitro/in vivo antitumor efficacy of aptamer-targeted Ecoflex<sup>®</sup> nanoparticles for docetaxel delivery in ovarian cancer