Biodegradable Polymers for Emerging Clinical Use in Tissue Engineering

Verma, Shalini; Garkhal, Kalpna; Mittal, Anupama; Kumar, Neeraj

doi:10.1002/9781118015810.ch16

Cited by 12 publications

(7 citation statements)

References 234 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Tissue engineering approaches involving a combination of growth factors, cells and biomaterial scaffolds have emerged as a promising tool for addressing the drawbacks of current clinical strategies for bone regeneration. [1][2][3] The combined delivery of growth factors and cells using biomaterial carriers can enhance and accelerate functional bone formation by providing localized release of the factors in a spatiotemporal manner and by promoting osteoprogenitor cellular function for accelerated tissue regeneration. 4,5 These combination approaches are of special significance as most of the available biomaterials, though osteoconductive in nature, lack osteoinductivity and osteogenic activity.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of the donor cell contribution in rhBMP‐2 mediated bone formation with chitosan thermogels using fluorescent protein reporter mice

Gohil

Kuo

Adams

et al. 2016

J Biomedical Materials Res

View full text Add to dashboard Cite

Our current understanding regarding the contribution of donor cells in growth factor and cell based tissue regeneration strategies is limited. The present study attempts to utilize fluorescent protein reporter mice [Col3.6Topaz (enhanced yellow fluorescent protein, EYFP) as host and Col3.6Cyan (enhanced cyan fluorescent protein, ECFP) as donor] to determine donor cell contribution in bone regeneration using a bilateral calvarial defect model. Thermogelling chitosan hydrogels (Chi-AHP) were used as bone marrow stromal cell (BMSC) delivery vehicle in the presence and absence of recombinant human bone morphogenetic protein-2 (rhBMP-2). Co-delivery of rhBMP-2 and donor BMSCs led to a significant increase in bone formation indicating the potential of using the combination approach for improved regeneration. On a cellular level, presence of rhBMP-2 resulted in an increased host cell derived osteoblast infiltration at 8 weeks. However, the new mineralized tissue presented two distinct morphological features based on its cellular origin. Regenerated tissue associated with ECFP positive donor cells showed a woven bone-like structure with diffuse alizarin complexone (AC) label and minimal tartrate resistant acid phosphatase (TRAP) activity, indicating the presence of immature early osteoblast cells depositing mineralized tissue without progressing to a mature lamellar bone. Host derived bone showed sharp mineralization line (AC), strong alkaline phosphatase (ALP) and TRAP activity, indicating the presence of actively mineralizing and remodeling, mature lamellar bone matrix. The study demonstrated the remarkable potential of transgenic reporters to improve our understanding of donor cell contribution during bone formation.

show abstract

Section: Introductionmentioning

confidence: 99%

Evaluation of the donor cell contribution in rhBMP‐2 mediated bone formation with chitosan thermogels using fluorescent protein reporter mice

Gohil

Kuo

Adams

et al. 2016

J Biomedical Materials Res

View full text Add to dashboard Cite

show abstract

“…These polyesters hydrolyze nonenzymatically into their monomer and are easily resorbed by metabolic pathways (7). They were initially reported as resorbable sutures with controlled release and in tissue engineering (8). PGA was one of the first materials of its kind studied for biomedical applications.…”

Section: Poly(α-hydroxy Acids)mentioning

confidence: 99%

Biodegradable Polymers: Medical Applications

Kunduru

Basu

Domb

2016

Encyclopedia of Polymer Science and Technology

View full text Add to dashboard Cite

Biodegradable polymers are the “green materials” of the future. They break down into smaller chemical fragments and are finally eliminated from the body. Biodegradable polymers have been used for more than 50 years with diverse applications such as surgical sutures, wound dressings, tissue regeneration, enzyme immobilization, controlled drug delivery and gene delivery, tissue engineering scaffold, cryopreservation, nanotechnology, medical implants and devices, prosthetics, augmentation, cosmetics, sanitation products, coatings, adhesives, and many more. This article reviews synthetic (esters, amides, ethers, urethanes) and natural (polysaccharides and proteins) biodegradable polymers, highlighting their biomedical applications.

show abstract

“…As a result of degradation, the hydrogel mass decreases and its mechanical stability is reduced. These properties could be advantageous for cell carrier applications [5,52], controlled release of drugs and growth factors [53,54], or tissue regeneration studies [55,56]. …”

Section: Hydrogels For Cell Encapsulationmentioning

confidence: 99%

Microscale Strategies for Generating Cell-Encapsulating Hydrogels

Selimović

Bae

et al. 2012

Polymers

View full text Add to dashboard Cite

Hydrogels in which cells are encapsulated are of great potential interest for tissue engineering applications. These gels provide a structure inside which cells can spread and proliferate. Such structures benefit from controlled microarchitectures that can affect the behavior of the enclosed cells. Microfabrication-based techniques are emerging as powerful approaches to generate such cell-encapsulating hydrogel structures. In this paper we introduce common hydrogels and their crosslinking methods and review the latest microscale approaches for generation of cell containing gel particles. We specifically focus on microfluidics-based methods and on techniques such as micromolding and electrospinning.

show abstract

Biodegradable Polymers for Emerging Clinical Use in Tissue Engineering

Cited by 12 publications

References 234 publications

Evaluation of the donor cell contribution in rhBMP‐2 mediated bone formation with chitosan thermogels using fluorescent protein reporter mice

Evaluation of the donor cell contribution in rhBMP‐2 mediated bone formation with chitosan thermogels using fluorescent protein reporter mice

Biodegradable Polymers: Medical Applications

Microscale Strategies for Generating Cell-Encapsulating Hydrogels

Contact Info

Product

Resources

About