Biodegradable polymer network encapsulated polyplex for DNA delivery

Petrov, Petar; Ivanova, Nadya I.; Apostolova, Margarita D.; Tsvetanov, Christo B.

doi:10.1039/c3ra21890b

Cited by 13 publications

(15 citation statements)

References 36 publications

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“…To avoid undesirable rearrangement into large microgel particles, the initial polyplex nanoparticles were stabilized by coating them with a cross-linked polymeric shell. A similar approach has recently been employed to prepare polymeric nanocapsules , and cover complexes of DNA and cationic copolymers. ,, This method employs heterophase radical copolymerization that occurs on the surface of the (polyplex) particles. The resulting shell acts as an envelope (i.e., a physical barrier) that provides mechanical strength and keeps the initial particles tight, preventing substantial swelling and eventual disintegration.…”

Section: Resultsmentioning

confidence: 99%

Partially Hydrolyzed Poly(n-propyl-2-oxazoline): Synthesis, Aqueous Solution Properties, and Preparation of Gene Delivery Systems

Mees

Haladjova²,

Momekova

et al. 2016

Biomacromolecules

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Random copolymers of n-propyl-2-oxazoline and ethylenimine (PPrOx-PEI) were prepared by partial acidic hydrolysis of poly(n-propyl-2-oxazoline) (PPrOx). Dynamic and electrophoretic light scattering and diffusion-ordered NMR spectroscopy were utilized to investigate aqueous solution properties of the copolymers. Above a specific cloud point temperature, well-defined nanoparticles were formed. The latter consisted of a core composed predominantly of PPrOx and a thin positively charged shell from PEI moieties that mediated formation of polyplexes with DNA. The polyplexes were prepared at 65 °C at varying N/P (amine-to-phosphate groups) ratios. They underwent structural changes upon temperature variations 65-25-37 °C depending on N/P. At N/P < 2, the polyplex particles underwent minor changes because of formation of a surface layer of DNA that acted as a barrier and prevented swelling and disintegration of the initial particles. Dramatic rearrangements at N/P ≥ 2 resulting in large swollen microgel particles were overcome by coating of the polyplex particles with a cross-linked polymeric shell. The shell retained the colloidal stability and preserved the physicochemical parameters of the initial polyplex particles while it reduced the high surface potential values. Progressive loss of cytotoxicity upon complexation with DNA and coating of polyplex particles was displayed.

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Section: Resultsmentioning

confidence: 99%

Partially Hydrolyzed Poly(n-propyl-2-oxazoline): Synthesis, Aqueous Solution Properties, and Preparation of Gene Delivery Systems

Mees

Haladjova²,

Momekova

et al. 2016

Biomacromolecules

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“…A large number of cationic polymers such as polyethylenimine, − poly( l -lysine), − poly(dimethylaminoethyl methacrylate), − chitosan, , etc., have been used to form polyplexes. Although their transfection efficiencies have typically been found to be satisfactory, cytotoxicity issues often limit their clinical use. , Nevertheless, the use of synthetic polymers offers a number of advantages and has no limitations in the possibility of polymer structure modification or attachment of ligands for cell-specific targeting.…”

Section: Introductionmentioning

confidence: 99%

Poly(vinyl benzyl trimethylammonium chloride) Homo and Block Copolymers Complexation with DNA

et al. 2016

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In this work we focus on the use of novel homo and block copolymers based on poly(vinyl benzyl trimethylammonium chloride) as gene delivery vectors. The homopolymers and block copolymers were synthesized by RAFT polymerization schemes and molecularly characterized. DNA/polymer complexes (polyplexes) in a wide range of N/P (amino-to-phosphate groups) ratios were prepared. The ability of the novel polymers to form complexes with linear DNA was investigated by light scattering, zeta potential, and ethidium bromide fluorescence quenching measurements. The resulting polyplexes were in the size range of 80-300 nm and their surface potential changed from negative to positive depending on the N/P ratio. The stability of polyplexes was monitored by changes in their hydrodynamic parameters in the presence of salt. The novel vector systems were visualized by transmission electron microscopy. The influence of factors such as molar mass, content, and chemical structure of the polycationic moieties as well as presence of a hydrophilic poly[oligo(ethylene glycol) methacrylate] block on the structure and stability of the polyplexes, kinetics of their formation, and effectiveness of the (co)polymers to shrink and pack DNA was discussed.

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“…For instance, Petrov, et al. dispersed triblock copolymer PDMAEMA‐PPO‐PDMAEMA poly(dimethylaminoethyl methacrylate)‐poly(propylene oxide)‐poly(dimethylaminoethyl methacrylate) into deionised water at 4 °C and the temperature was then increase to 25 ° C . Upon temperature change, the phase transition of PPO components from hydrophilic to hydrophobic occurred, leading to the formation of polymeric micelles with the PPO core surrounded by hydrophilic PDMAEMA fragments.…”

Section: Common Methods For Preperation Of Polymeric Hollow Capsulesmentioning

confidence: 99%

“…For instance, Petrov, et al dispersed triblock copolymer PDMAEMA-PPO-PDMAEMA poly(dimethylaminoethyl methacrylate)-poly(propylene oxide)-poly(dimethylaminoethyl methacrylate) into deionised water at 4 °C and the temperature was then increase to 25°C. [49] Upon temperature change, the phase transition of PPO components from hydrophilic to hydrophobic occurred, leading to the formation of polymeric micelles with the PPO core surrounded by hydrophilic PDMAEMA fragments. Owing to high positive net charge on the surface (ξ = 51.7 mV), these micelles were used for the adhesion of negatively charged plasmid DNA (ξ = À 32.3 mV) prior to the seeded radical polymerization of N-isopropylacrylamide.…”

Section: Layer-by-layer (Lbl) Self-assemblymentioning

confidence: 99%

Current Advances of Hollow Capsules as Controlled Drug Delivery Systems

Tran

Bhave

2020

ChemistrySelect

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Polymeric hollow capsules have attracted increasing interest for the development of controlled drug delivery systems due to their capacities to load high amount of drugs and be able to finely tune the drug release profile. Various methods and strategies for capsule preparation and drug loading have been developed over time. Besides that, the controlled drug release from hollow capsules upon external and internal triggers is of great interest and has been a research focus in this area. This article aims to give the most recent progress review on hollow capsule based controlled drug delivery systems with a highlight on strategies being used for drug loading and stimuli-release. In detail, the fabrication of hollow capsules using templateassisted or template-free methods will be introduced first. This will be followed by current strategies for pre-loading and postloading of therapeutic agents into capsules. The article will then be particularly focused on discussing diverse drug-release systems corresponding to various stimuli conditions including temperature, pH, ultrasound, light irradiation and enzyme degradation.

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Biodegradable polymer network encapsulated polyplex for DNA delivery

Cited by 13 publications

References 36 publications

Partially Hydrolyzed Poly(n-propyl-2-oxazoline): Synthesis, Aqueous Solution Properties, and Preparation of Gene Delivery Systems

Partially Hydrolyzed Poly(n-propyl-2-oxazoline): Synthesis, Aqueous Solution Properties, and Preparation of Gene Delivery Systems

Poly(vinyl benzyl trimethylammonium chloride) Homo and Block Copolymers Complexation with DNA

Current Advances of Hollow Capsules as Controlled Drug Delivery Systems

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