Biodegradable Nanoparticles Enhanced Adhesiveness of Mussel‐Like Hydrogels at Tissue Interface

Abstract

Popular bioadhesives, such as fibrin, cyanoacrylate, and albumin-glutaraldehyde based materials, have been applied for clinical applications in wound healing, drug delivery, and bone and soft tissue engineering; however, their performances are limited by weak adhesion strength and rapid degradation. In this study a mussel-inspired, nanocomposite-based, biodegradable tissue adhesive is developed by blending poly(lactic-co-glycolic acid) (PLGA) or N-hydroxysuccinimide modified PLGA nanoparticles (PLGA-NHS) with mussel-inspired alginate-dopamine polymer (Alg-Dopa). Adhesive strength measurement of the nanocomposites on porcine skin-muscle constructs reveals that the incorporation of nanoparticles in Alg-Dopa significantly enhances the tissue adhesive strength compared to the mussel-inspired adhesive alone. The nanocomposite formed by PLGA-NHS nanoparticles shows higher lap shear strength of 33 ± 3 kPa, compared to that of Alg-Dopa hydrogel alone (14 ± 2 kPa). In addition, these nanocomposites are degradable and cytocompatible in vitro, and elicit in vivo minimal inflammatory responses in a rat model, suggesting clinical potential of these nanocomposites as bioadhesives.

Liquid Copolymers as Biodegradable Surgical Sealant

“…The adhesion mechanism of this system will be based on the interaction between the carboxy-Nhydroxysuccinimide (NHS) of the polymer and amine groups presented by the tissue. [16] The cohesion mechanism will be based on a lack of solvent and on the interaction between the NHS of the four-armed polyethylene glycol-poly (lactic-co-glycolic acid)-NHS (PEG 4 -PLGA-NHS) and the primary amine of the complementary prepolymers, four-armed polyethylene glycol-NH 2 (PEG 4 -NH 2 ). The conversion ratio of activated NHS-ester carboxyl groups with amine nucleophiles to amide bonds is up to 100%.…”

Engineering a naturally-derived adhesive and conductive cardiopatch

“…Lastly, although cardiac patches hold great potential for the treatment of MI, their integration with the myocardium by means of sutures or staples may lead to further trauma and blockage of blood supply, bleeding, and infection. While different alternatives such as medical grade cyanoacrylates [32] and other bioadhesives [33][34][35] have been developed to adhere these patches on the heart, many of these approaches are associated with cytotoxicity and high stiffness (e.g cyanoacrylates), low adhesion and structural stability (e.g. fibrin-based bioadhesives), as well as failure to provide a biomimetic environment that allows for tissue regeneration [12,[36][37][38].…”

Enhancing Tissue Adhesion and Osteoblastic Differentiation of MC3T3‐E1 Cells on Poly(aryl ether ketone) by Chemically Anchored Hydroxyapatite Nanocomposite Hydrogel Coating

“…The adhesion enhancement effect of nanoparticles could be attributed to the following four aspects: First, the nano‐HA could be adsorbed to the surface of the tissue and broken under the action of the gel network conduction force, which consumed a lot of energy to inhibit the propagation and growth of cracks; [ 48,49 ] Second, the platelet nanoparticles used in this experiment had a significant enhancement of adhesion over spherical and cylindrical nanoparticles, owing to the much higher surface‐to‐volume ratio and the easier interaction with the interfaces; [ 50 ] Third, the incorporation of nanoparticles to the hydrogel effectively reduced its penetration into the tissue and made cohesiveness increase to some extent. [ 51,52 ] This was substantiated by the fact that the addition of nano‐HA caused a large increase in E ′ and tan δ (Figure S7a,b, Supporting Information), which meant that a higher activation energy for the breaking of cross‐links was needed; Fourth, the adsorption of PAA chains on big nano‐HA particles presumably mainly produced a “mushroom” region consisting of a sparse number of extended loops or tails, resulting in better adhesion. [ 53 ] To add, the decrease in the lap shear strength of GN 1 AH 2 was due to the agglomeration caused by excess nano‐HA and increases of tensile strength and toughness, which hindered the close contact between the hydrogel and tissue interface and the occurrence of physical and chemical reactions.…”