Biodegradable IPNs based on oxidized alginate and dextran-HEMA for controlled release of proteins

Pescosolido, Laura; Piro, Teresa; Vermonden, Tina; Coviello, Tommasina; Alhaique, Franco; Hennink, Wim E.; Matricardi, Pietro

doi:10.1016/j.carbpol.2011.04.033

Cited by 43 publications

(22 citation statements)

References 33 publications

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“…(Dragan, 2014) However, most of the hybrid IPNs hydrogels, reported in literature, are obtained by either combining various polysaccharides or synthetic polymers and proteins with synthetic polymers. (Dragan, 2014;La Gatta, Schiraldi, Esposito, D'Agostino, & De Rosa, 2009;Peng, Yu, Mi, & Shyu, 2006;Pescosolido et al, 2011) Only a few papers report on the combination of proteins and polysaccharides for the construction of (semi)-IPNs. (Cui, Jia, Guo, Liu, & Zhu, 2014;Y.…”

Section: Introductionmentioning

confidence: 98%

Gelatin- and starch-based hydrogels. Part A: Hydrogel development, characterization and coating

Nieuwenhove

Salamon

Peters

et al. 2016

Carbohydrate Polymers

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The present work aims at constructing the ideal scaffold matrix of which the physico-chemical properties can be altered according to the targeted tissue regeneration application. Ideally, this scaffold should resemble the natural extracellular matrix (ECM) as close as possible both in terms of chemical composition and mechanical properties. Therefore, hydrogel films were developed consisting of methacrylamide-modified gelatin and starch-pentenoate building blocks because the ECM can be considered as a crosslinked hydrogel network consisting of both polysaccharides and structural, signaling and cell-adhesive proteins. For the gelatin hydrogels, three different substitution degrees were evaluated including 31%, 72% and 95%. A substitution degree of 32% was applied for the starch-pentenoate building block. Pure gelatin hydrogels films as well as interpenetrating networks with gelatin and starch were developed. Subsequently, these films were characterized using gel fraction and swelling experiments, high resolution-magic angle spinning (1)H NMR spectroscopy, rheology, infrared mapping and atomic force microscopy. The results indicate that both the mechanical properties and the swelling extent of the developed hydrogel films can be controlled by varying the chemical composition and the degree of substitution of the methacrylamide-modified gelatin applied. The storage moduli of the developed materials ranged between 14 and 63kPa. Phase separation was observed for the IPNs for which separated starch domains could be distinguished located in the surrounding gelatin matrix. Furthermore, we evaluated the affinity of aggrecan for gelatin by atomic force microscopy and radiolabeling experiments. We found that aggrecan can be applied as a bioactive coating for gelatin hydrogels by a straightforward physisorption procedure. Thus, we achieved distinct fine-tuning of the physico-chemical properties of these hydrogels which render them promising candidates for tissue engineering approaches.

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Section: Introductionmentioning

confidence: 98%

Gelatin- and starch-based hydrogels. Part A: Hydrogel development, characterization and coating

Nieuwenhove

Salamon

Peters

et al. 2016

Carbohydrate Polymers

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“…Alginate polymer can be cross‐linked with multivalent metal cations (Ca 2+ cations are the most utilized cross‐linkers) producing a hydrogel capable of encapsulation and then controlled release of various biomolecules, including proteins/enzymes, DNA, and other (bio)molecular species, for example, drugs . The Ca 2+ ‐cross‐linked alginate hydrogel has been used for continuous slow release (leakage) of the entrapped species.…”

Section: Introductionmentioning

confidence: 99%

Glucose‐Triggered Insulin Release from Fe³⁺‐Cross‐linked Alginate Hydrogel: Experimental Study and Theoretical Modeling

et al. 2017

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We study the mechanisms involved in the release, triggered by the application of glucose, of insulin entrapped in Fe -cross-linked alginate hydrogel particles further stabilized with a polyelectrolyte. Platelet-shaped alginate particles are synthesized containing enzyme glucose oxidase conjugated with silica nanoparticles, which are also entrapped in the hydrogel. Glucose diffuses in from solution, and production of hydrogen peroxide is catalyzed by the enzyme within the hydrogel. We argue that, specifically for the Fe -cross-linked systems, the produced hydrogen peroxide is further converted to free radicals via a Fenton-type reaction catalyzed by the iron cations. The activity of free radicals, as well as the reduction of Fe by the enzyme, and other mechanisms contribute to the decrease in density of the hydrogel. As a result, while the particles remain intact, void sizes increase and release of insulin ensues and is followed experimentally. A theoretical description of the involved processes is proposed and utilized to fit the data. It is then used to study the long-time properties of the release process that offers a model for designing new drug-release systems.

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“…ODex was synthesized according to a literature reported procedure with slight modification . Briefly, 2.00 g of dextran was dissolved into 50 mL of sodium dihydrogen phosphate buffer solution (1/15 m ).…”

Section: Methodsmentioning

confidence: 99%

Injectable Polysaccharide Hydrogels as Biocompatible Platforms for Localized and Sustained Delivery of Antibiotics for Preventing Local Infections

Yuan

et al. 2016

Macromolecular Bioscience

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Biocompatible and antibacterial hydrogels have received increasing attention for preventing local bacterial infections. In this study, a type of polysaccharide hydrogels is prepared via the Schiff-based reaction at physiological conditions. The gelation time and mechanical property of the hydrogels are found to be dependent on the polysaccharide concentration and the polysaccharide weight ratio. 3-(4,5-Dimethyl-thiazol-2-yl)-2,5-diphenyl tetrazolium bromide assay and live/dead assay indicate that the hydrogels display nontoxicity in vitro. After subcutaneous injection into rats, the hydrogels exhibit an acceptable biocompatibility in vivo. Furthermore, the bacterial inhibition tests by shaking flask method and agar disc-diffusion method demonstrate that the ceftriaxone-sodium-loaded hydrogels have remarkable antibacterial properties in vitro. The in vivo anti-infective tests further display that the antibiotic-loaded hydrogels display excellent anti-infective efficacies in both superficial and deep tissue infection. Consequently, the injectable and biocompatible polysaccharide hydrogels may serve as promising platforms for localized, sustained delivery of antibiotics for preventing local infections.

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Biodegradable IPNs based on oxidized alginate and dextran-HEMA for controlled release of proteins

Cited by 43 publications

References 33 publications

Gelatin- and starch-based hydrogels. Part A: Hydrogel development, characterization and coating

Gelatin- and starch-based hydrogels. Part A: Hydrogel development, characterization and coating

Glucose‐Triggered Insulin Release from Fe³⁺‐Cross‐linked Alginate Hydrogel: Experimental Study and Theoretical Modeling

Injectable Polysaccharide Hydrogels as Biocompatible Platforms for Localized and Sustained Delivery of Antibiotics for Preventing Local Infections

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Biodegradable IPNs based on oxidized alginate and dextran-HEMA for controlled release of proteins

Cited by 43 publications

References 33 publications

Gelatin- and starch-based hydrogels. Part A: Hydrogel development, characterization and coating

Gelatin- and starch-based hydrogels. Part A: Hydrogel development, characterization and coating

Glucose‐Triggered Insulin Release from Fe3+‐Cross‐linked Alginate Hydrogel: Experimental Study and Theoretical Modeling

Injectable Polysaccharide Hydrogels as Biocompatible Platforms for Localized and Sustained Delivery of Antibiotics for Preventing Local Infections

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Product

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Glucose‐Triggered Insulin Release from Fe³⁺‐Cross‐linked Alginate Hydrogel: Experimental Study and Theoretical Modeling