Biodegradable cationic PEG–PEI–PBLG hyperbranched block copolymer: synthesis and micelle characterization

Tian, Hua Yu; Deng, Chao; Lin, Hao; Sun, Jingru; Deng, Mingxiao; Chen, Xuesi; Jing, Xiabin

doi:10.1016/j.biomaterials.2004.11.002

Cited by 207 publications

(153 citation statements)

References 28 publications

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“…It should be noted that pNPs have lower CMC than most reported vectors, such as PEGylated vectors, whose magnitude of CMC is ~10 -2 mg/mL, 8,30,31 suggesting that pNPs are less susceptible to disassembly upon dilution. Thus, during in vivo applications, when subject to large dilution, pNPs can provide continuous protection to loaded genes, which ensures that genes can survive from enzyme digestion and successfully reach the expected sites with bioactivity.…”

mentioning

confidence: 93%

Stability and toxicity of empty or gene-loaded lipopolysaccharide-amine nanopolymersomes

Wang¹,

Chen²,

Wang³

et al. 2015

IJN

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Successful in vivo gene delivery mediated by nonviral vectors requires efficient extracellular and intracellular gene delivery, but few studies have given attention to the former. That is why numerous gene delivery systems have succeeded in vitro, while the expected clinical success has not come about. To realize efficient extracellular gene delivery, the stability of vectors and/or their complexes with genes in body fluids is first required, which prevents loaded genes from premature unloading and degradation. Furthermore, the storage stability of vectors under common conditions is important for their widespread applications. Lipopolysaccharide-amine nanopolymersomes (NPs), a gene vector developed by our group recently, have higher than 95% in vitro transfection efficiency in mesenchymal stem cells when delivering pEGFP , and induce significant angiogenesis in zebrafish when delivering plasmid encoding vascular endothelial growth factor deoxyribonucleic acid ( pVEGF ). To reveal their extracellular delivery ability and storage stability, in this study their stability in various simulant physiological environments and storage conditions was systematically studied by monitoring their changes in disassembly, size, zeta potential, and transfection efficiency. Additionally, damage to the mitochondria of mesenchymal stem cells was evaluated. Results show that NPs and plasmid deoxyribonucleic acid (pDNA)-loaded NPs (pNPs) have acceptable stability against dilution, anions, salts, pH, enzyme, and serum, presumably assuring their efficient extracellular delivery in vivo. Moreover, both the lyophilized NPs at room temperature and NP/pNP solution at 4°C have high storage stability, and pNPs show low damage to the mitochondria. The acceptable stability of NPs combined with compatibility and efficient gene transfection highlight their huge potential in the clinic as a gene delivery vector.

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mentioning

confidence: 93%

Stability and toxicity of empty or gene-loaded lipopolysaccharide-amine nanopolymersomes

Wang¹,

Chen²,

Wang³

et al. 2015

IJN

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“…Compared with linear counterparts, star-shaped polymers with the same molecular weight have showed higher transfection efficiency. [34][35][36] Moreover, compared with linear polymers, the amphiphilic star-shaped polymers show relatively small hydrodynamic radius, and low solution and melt viscosities. 35 Plenty of star-shaped polycations containing a biocompatible core display excellent performance in gene therapy.…”

Section: Feng Et Almentioning

confidence: 99%

“…[34][35][36] Moreover, compared with linear polymers, the amphiphilic star-shaped polymers show relatively small hydrodynamic radius, and low solution and melt viscosities. 35 Plenty of star-shaped polycations containing a biocompatible core display excellent performance in gene therapy. For example, Yang et al synthesized a bioreducible star-shaped gene carrier consisting of a biocompatible polyhedral oligomeric silsesquioxane core and eight disulfide-linked poly(2-dimethylamino)ethyl methacrylate arms.…”

Section: Feng Et Almentioning

confidence: 99%

Co-self-assembly of cationic microparticles to deliver pEGFP-ZNF580 for promoting the transfection and migration of endothelial cells

Feng¹,

Guo²,

Li³

et al. 2016

IJN

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“…22 Moreover, the folate receptor was reported to be overexpressed on human esophageal cancer cells. 23 In our previous work, the folate was attached to a triblock copolymer, methoxy-poly(ethylene glycol)-b-poly (L-lactide)-b-poly(L-lysine), [24][25][26][27] and preferred uptake by HeLa cells and accumulation in mouse hepatoma H22 xenograft tumors were observed. In this research, it was attached to [MPEG-b-P(LA-co-DHP)] while paclitaxel was conjugated with MPEG-b-P(LA-co-MCC), and the two micelles were coassembled into composite micelles containing folic acid and paclitaxel.…”

Section: Introductionmentioning

confidence: 98%

Antitumor activity of folate-targeted, paclitaxel-loaded polymeric micelles on a human esophageal EC9706 cancer cell line

Wu¹,

Zheng²,

Wang³

et al. 2012

IJN

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Background: Esophageal cancer is recognized as one of the most refractory pernicious diseases. In addition, it is an aggressive malignancy with a propensity for local progression and distant dissemination. Because of the poor long-term prognosis for patients with esophageal cancer, increasing attention has focused on the integration of targeted agents into current therapeutics. Nevertheless, there have been few studies reported concerning the therapeutic efficacy of paclitaxel-conjugated polymeric micelles in human esophageal cancer in vivo. Therefore, the aim of this research was to investigate the tumor inhibition effect of composite micelles containing folic acid and paclitaxel on the human esophageal EC9706 cancer cell line. Methods and results:Intravenous administration of folate-targeted, paclitaxel-loaded micelles was demonstrated to be more efficient in inhibiting subcutaneous xenograft tumors and extending the survival rate of tumor-bearing nude mice than free paclitaxel and plain paclitaxel micelles at an equivalent paclitaxel dose of 20 mg/kg, which was further backed up by flow cytometry, TUNEL, and expression of apoptosis-related proteins, including Bax, Bcl2, and caspase 3 in this study. Conclusion:The folate-mediated paclitaxel-loaded polymeric micelle is a promising agent for the treatment of human esophageal cancer.

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Biodegradable cationic PEG–PEI–PBLG hyperbranched block copolymer: synthesis and micelle characterization

Cited by 207 publications

References 28 publications

Stability and toxicity of empty or gene-loaded lipopolysaccharide-amine nanopolymersomes

Stability and toxicity of empty or gene-loaded lipopolysaccharide-amine nanopolymersomes

Co-self-assembly of cationic microparticles to deliver pEGFP-ZNF580 for promoting the transfection and migration of endothelial cells

Antitumor activity of folate-targeted, paclitaxel-loaded polymeric micelles on a human esophageal EC9706 cancer cell line

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